LOCUS       sequence-139930-       11602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This plasmid was derived from pZY101 by inserting a GUS-intron 
            reporter gene cassette into multiple cloning sites. This plasmid is 
            recommended for use as empty control for service event request..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11602)
  AUTHORS   Zeng P, Vadnais DA, Zhang Z, Polacco JC
  TITLE     Refined glufosinate selection in Agrobacterium-mediated 
            transformation of soybean [Glycine max (L.) Merrill].
  JOURNAL   Plant Cell Rep. 2004 Feb;22(7):478-82. Epub 2003 Sep 30.
  PUBMED    15034747
REFERENCE   2  (bases 1 to 11602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell 
            Rep."; date: "2004-02"; volume: "22(7)"; pages: "478-82. Epub 2003 
            Sep 30"
COMMENT     Sequence Label: pZY102
FEATURES             Location/Qualifiers
     source          1..11602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1187..1816
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2250..3317
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      3383..3577
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3813..3835)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3921..4061
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3976..3995
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4076..4093)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4247..4835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4327..4346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5079..5870)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    6398..6422
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     6622..6644
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6636..6653
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6637..6653
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(7221..7769)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(7921..8265)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     intron          10083..10271
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     promoter        complement(10694..11038)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    11128..11184
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(11193..11209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11193..11209)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11206..11228)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11217..11233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11241..11247,11248..11265,11266..11271))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11286..11307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    11465..11489
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
       61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
      121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
      181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
      241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
      301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
      361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
      421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
      481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
      541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
      601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
      661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
      721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
      781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
      841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
      901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
      961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
     1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
     1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
     1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
     1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
     1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
     1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
     1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
     1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
     1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
     1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
     1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
     1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
     1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
     1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
     1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
     1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
     1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
     2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
     2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
     2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
     2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
     2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
     2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
     3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
     4921 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
     4981 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg
     5041 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg
     5101 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
     5161 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
     5221 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
     5281 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
     5341 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
     5401 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
     5461 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
     5521 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
     5581 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
     5641 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
     5701 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
     5761 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
     5821 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac
     5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
     6001 aaacatgtca taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     6061 ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga
     6121 accgaacgag gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc
     6181 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
     6241 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
     6301 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
     6361 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
     6421 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
     6481 acgccgaatt gctctagcat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     6541 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     6601 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
     6661 taattcttca agacgtgctc aaatcactat ttccacaccc ctatatttct attgcactcc
     6721 cttttaactg ttttttatta caaaaatgcc ctggaaaatg cactcccttt ttgtgtttgt
     6781 ttttttgtga aacgatgttg tcaggtaatt tatttgtcag tctactatgg tggcccatta
     6841 tattaatagc aactgtcggt ccaatagacg acgtcgattt tctgcatttg tttaaccacg
     6901 tggattttat gacattttat attagttaat ttgtaaaacc tacccaatta aagacctcat
     6961 atgttctaaa gactaatact taatgataac aattttcttt tagtgaagaa agggataatt
     7021 agtaaatatg gaacaagggc agaagattta ttaaagccgc gtaagagaca acaagtaggt
     7081 acgtggagtg tcttaggtga cttacccaca taacataaag tgacattaac aaacatagct
     7141 aatgctccta tttgaatagt gcatatcagc ataccttatt acatatagat aggagcaaac
     7201 tctagctaga ttgttgagca gatctcggtg acgggcagga ccggacgggg cggtaccggc
     7261 aggctgaagt ccagctgcca gaaacccacg tcatgccagt tcccgtgctt gaagccggcc
     7321 gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg
     7381 ttgggcagcc cgatgacagc gaccacgctc ttgaagccct gtgcctccag ggacttcagc
     7441 aggtgggtgt agagcgtgga gcccagtccc gtccgctggt ggcgggggga gacgtacacg
     7501 gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc aggggcccgc gtaggcgatg
     7561 ccggcgacct cgccgtccac ctcggcgacg agccagggat agcgctcccg cagacggacg
     7621 aggtcgtccg tccactcctg cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc
     7681 tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg
     7741 atgtcggccg ggcgtcgttc tgggctcatg gtagatcccc cgttcgtaaa tggtgaaaat
     7801 tttcagaaaa ttgcttttgc tttaaaagaa atgatttaaa ttgctgcaat agaagtagaa
     7861 tgcttgattg cttgagattc gtttgttttg tatatgttgt gttgagaatt aattctcgag
     7921 gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg
     7981 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
     8041 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
     8101 ccactgtcgg cagaggcatc ttcaacgatg gcctttcctt tatcgcaatg atggcatttg
     8161 taggagccac cttccttttc cactatcttc acaataaagt gacagatagc tgggcaatgg
     8221 aatccgagga ggtttccgga tattaccctt tgttgaaaag tctcaattgc cctttggtct
     8281 tctgagactg tatctttgat atttttggag tagacaagcg tgtcgtgctc caccatgttg
     8341 acctgcaggc atgcaagctc aagcttgcat gcctgcaggt cactggattt tggttttagg
     8401 aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt
     8461 atatgctcaa cacatgagcg aaaccctata agaaccctaa ttcccttatc tgggaactac
     8521 tcacacatta ttctggagaa aaatagagag agatagattt gtagagagag actggtgatt
     8581 tttgcgccgg gtaccgagct cggtagcaat tcccgaggct gtagccgacg atggtgcgcc
     8641 aggagagttg ttgattcatt gtttgcctcc ctgctgcggt ttttcaccga agttcatgcc
     8701 agtccagcgt ttttgcagca gaaaagccgc cgacttcggt ttgcggtcgc gagtgaagat
     8761 ccctttcttg ttaccgccaa cgcgcaatat gccttgcgag gtcgcaaaat cggcgaaatt
     8821 ccatacctgt tcaccgacga cggcgctgac gcgatcaaag acgcggtgat acatatccag
     8881 ccatgcacac tgatactctt cactccacat gtcggtgtac attgagtgca gcccggctaa
     8941 cgtatccacg ccgtattcgg tgatgataat cggctgatgc agtttctcct gccaggccag
     9001 aagttctttt tccagtacct tctctgccgt ttccaaatcg ccgctttgga cataccatcc
     9061 gtaataacgg ttcangcaca gcacatcaaa gagatcgctg atggtatcgg tgtgagcgtc
     9121 gcanaacatt acattgacgc angtgatcgg acgcgtcggg tcgagtttac gcgttgcttc
     9181 cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg gtatccggtt cgttggcaat
     9241 actccacatc accacgcttg ggtggttttt gtcacgcgct atcagctctt taatcgcctg
     9301 taagtgcgct tgctgagttt ccccgttgac tgcctcttcg ctgtacagtt ctttcggctt
     9361 gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag ccgacagcag cagtttcatc
     9421 antcaccacg atgccatgtt catctgccca gtcgagcatc tcttcagcgt aagggtaatg
     9481 cgaggtacgg taggagttgg ccccaatcca gtccattaat gcgtggtcgt gcaccatcag
     9541 cacgttatcg aatcctttgc cacgcaagtc cgcatcttca tgacgaccaa agccagtaaa
     9601 gtagaacggt ttgtggttaa tcaggaactg ttcgcccttc actgccactg accggatgcc
     9661 gacgcgaagc gggtagatat cacactctgt ctggcttttg gctgtgacgc acagttcata
     9721 gagataacct tcacccggtt gccagaggtg cggattcacc acttgcaaag tcccgctagt
     9781 gccttgtcca gttgcaacca cctgttgatc cgcatcacgc agttcaacgc tgacatcacc
     9841 attggccacc acctgccagt caacagacgc gtggttacag tcttgcgcga catgcgtcac
     9901 cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag agcattacgc tgcgatggat
     9961 tccggcatag ttaaagaaat catggaagta agactgcttt ttcttgccgt tttcgtcggt
    10021 aatcaccatt cccggcggga tagtctgcca gttcagttcg ttgttcacac aaacggtgat
    10081 acctgcacat caacaaattt tggtcatata ttagaaaagt tataaattaa aatatacaca
    10141 cttataaact acagaaaagc aattgctata tactacattc ttttattttg aaaaaaatat
    10201 ttgaaatatt atattactac taattaatga taattattat atatatatca aaggtagaag
    10261 cagaaactta cgtacacttt tcccggcaat aacatacggc gtgacatcgg cttcaaatgg
    10321 cgtatagccg ccctgatgct ccatcacttc ctgattattg acccacactt tgccgtaatg
    10381 agtgaccgca tcgaaacgca gcacgatacg ctggcctgcc caacctttcg gtataaagac
    10441 ttcgcgctga taccagacgt tgcccgcata attacgaata tctgcatcgg cgaactgatc
    10501 gttaaaactg cctggcacag caattgcccg gctttcttgt aacgcgcttt cccaccaacg
    10561 ctgaccaatt ccacagtttt cgcgatccag actgaatgcc cacaggccgt cgagtttttt
    10621 gatttcacgg gttggggttt ctacaggacg taacataagg gactgaccta cccggggatc
    10681 ctctagagtc gaggtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
    10741 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
    10801 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgttcctcg tgggtggggg
    10861 tccatctttg ggaccactgt cggtagaggc atcttgaacg atagcctttc ctttatcgca
    10921 atgatggcat ttgtagaagc catcttcctt ttctactgtc ctttcgatga agtgacagat
    10981 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
    11041 agccctctgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
    11101 ctccaccatg ttgacctgca ggcatgcaag cttgcatgcc tgcaggtcga ctctagagga
    11161 tccccgggta ccgagctcga attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt
    11221 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
    11281 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    11341 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    11401 cgtattggag cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag
    11461 tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg
    11521 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
    11581 acagggttcc cctcgggatc aa
//
