LOCUS       sequence-139936-        1098 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 based UAS modified with a CCR5TC binding site upstream of it, 
            driving PSD95-FingR fused to GFP, CCR5TC zinc finger, and KRAB(A) 
            repressor domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1098)
  TITLE     Bonkowsky lab FingR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: zcUAS:PSD95.FingR-GFP-ZFC(CCR5TC)-KRAB(A)
FEATURES             Location/Qualifiers
     source          1..1098
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..77)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(351..375)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        375..393
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     430..446
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     430..446
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    1013..1024
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc ccagatctac tggagccgct acccttatcg
       61 tcgtcatcct tgtaatcggt tccggtgcgg tagttgatgg agatcggcgg ccaggcggag
      121 cggtaggcgc tgaagatcgt gacggcgtac accgtgatgg tatagtcgac accaggtttc
      181 aggccgctga tggtagcagt ggacttgctg ccaggcacgg tgaattcctg gacagggcta
      241 ttgccaccag tttcaccgta ggtgatgcgg tagtagcgaa cgtggcgcaa gtggagcacc
      301 cagctgatct ggatgctggt tggtgatgct tccttgactt cgagcatggt agcctgcttt
      361 tttgtacaaa cttgaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt
      421 ggcggccgct ctagaactag tggatccccc gggctgcagg aattcgtgtg gaggagctca
      481 aagtgaggct gagacgcgat gggatccatt atataccctc tagagtcgac ccttgccgga
      541 ggacagtact ccgccagccc ggaggacagt actccgcctt gcgtcggagg acagtactcc
      601 gacctctagt gtcggaggac agtactccga ccttgccgga ggacagtact ccgcagcccg
      661 gaggacagta ctccgccttg cgtcggagga cagtactccg acctctagtg tcggaggaca
      721 gtactccgac cttgccggag gacagtactc cgccagcccg gaggacagta ctccgccttg
      781 gaggcctaag cttaccgtaa atagtccacc cattgacgtc aatggaaagt ccctattggc
      841 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
      901 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
      961 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtcg acgatgagga
     1021 tgactctagt caataatcaa tgtcgaccag gtggcacttt tcggggaaat gtgcgcggaa
     1081 cccctatttg tttatttt
//
