LOCUS       sequence-139948-        5033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gBT-I TCR beta chain cDNA in pCRII.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Mueller SN, Heath W, McLain JD, Carbone FR, Jones CM
  TITLE     Characterization of two TCR transgenic mouse lines specific for 
            herpes simplex virus.
  JOURNAL   Immunol Cell Biol. 2002 Apr;80(2):156-63.
  PUBMED    11940116
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunol 
            Cell Biol."; date: "2002-04"; volume: "80(2)"; pages: "156-63"
COMMENT     Sequence Label: pCRII-HSV2.3 TCR beta chain
FEATURES             Location/Qualifiers
     source          1..5033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        239..257
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     239..256
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1466..1485)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1467..1485)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1492..1509)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1492..1508)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1501..1523)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1649..2077
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1736..1755)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1946..1967
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             2421..3215
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2475..2494)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3085..3104
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(3233..3301,3302..4093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3451..3470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4264..4852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4753..4772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5006..5023
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc ggcttagatc tcacctatgc gtgactagta
      361 ggcagaattt gggatgcaca gacaaatgag aatagctctt cagaagttct gggagaagaa
      421 aatctgtatt aacacaatgt gttgtcagct cttgtattca tcttcacatc tggcttcatg
      481 aattctttct tttgaccata gccatcacca ccagtgtact gacaagcaca gcatacaggg
      541 tggctttccc tagcaggatc tcatagagga tggtggcaga caagacccct tgttgatagg
      601 atgctgaggt aatcccacag tctgctcggc cccaggcctc tgcactgatg ttctgtgtga
      661 caggtttggg tgagccctct ggccacttgt cctcctctga aagcccatgg aactgcactt
      721 ggcagcggaa gtggttgcga ggattgtgcc agaaggtagc agagaccctc aggcggctgc
      781 tcaggcagta gctataattg ctctccttgt aggcctgagg gtccgtgctg accccactgt
      841 ggacctcctt gccattcacc caccagctca gctccacgtg gtcagggaag aagcccctgg
      901 ccaagcacac gagggtagcc ttttgtttgt ttgcaatctc tgcttttgat ggctcaaaca
      961 aggagacctt gggtggagtc acatttctca gatcctctat taccaaaagc ctggtccctg
     1021 agccgaaggt gtagtcggag accccctgtc ctctgctggc acagaaatat acagctgtct
     1081 gagaaaggga agccaactcc agaatgagag agaaattctc ttggcttggt ctggaggcct
     1141 tgtacccatc agggatatct cctttctccg tgctgtcagc gacatatgag taatggatca
     1201 gcctcagccc atgccccgtg tcctgccgat accagtacat atagtcatgg ttattagtct
     1261 ggtgacagct caatgtcacc tttcctcctg ttactgccac cttgcttctt ggactttggg
     1321 tgactgcagc ctccatgtgt tttgcacaca ggagaatcaa aaccacaaag aagagtctgg
     1381 agcccatctc agaactaaag ccgaattctg cagatatcca tcacactggc ggccgctcga
     1441 gcatgcatct agagggccca attcgcccta tagtgagtcg tattacaatt cactggccgt
     1501 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     1561 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     1621 acagttgcgc agcctgaatg gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg
     1681 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     1741 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     1801 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     1861 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     1921 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     1981 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     2041 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaattca gggcgcaagg
     2101 gctgctaaag gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga
     2161 tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg
     2221 tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg
     2281 aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
     2341 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga
     2401 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     2461 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     2521 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     2581 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     2641 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     2701 tgggcgaagt gccggggcag gatctcctgt catcccacct tgctcctgcc gagaaagtat
     2761 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     2821 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     2881 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     2941 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     3001 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     3061 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     3121 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     3181 tcgccttcta tcgccttctt gacgagttct tctgaattga aaaaggaaga gtatgagtat
     3241 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3301 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3361 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3421 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3481 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3541 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3601 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3661 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3721 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3781 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3841 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3901 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3961 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4021 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4081 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4141 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4201 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4261 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4321 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4381 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     4441 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4501 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4561 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4621 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4681 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4741 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4801 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4861 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4921 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4981 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag
//
