LOCUS       sequence-139949-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a single-wavelength red-shifted genetically encoded calcium 
            indicator constructed from calmodulin and cp-mRuby.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Akerboom J, Carreras Calderon N, Tian L, Wabnig S, Prigge M, Tolo J,
            Gordus A, Orger MB, Severi KE, Macklin JJ, Patel R, Pulver SR, 
            Wardill TJ, Fischer E, Schuler C, Chen TW, Sarkisyan KS, Marvin JS, 
            Bargmann CI, Kim DS, Kugler S, Lagnado L, Hegemann P, Gottschalk A, 
            Schreiter ER, Looger LL
  TITLE     Genetically encoded calcium indicators for multi-color neural 
            activity imaging and combination with optogenetics.
  JOURNAL   Front Mol Neurosci. 2013;6:2. doi: 10.3389/fnmol.2013.00002. Epub 
            2013 Mar 4.
  PUBMED    23459413
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2013"; volume: "6:2. doi"; pages: " 
            10.3389/fnmol.2013.00002. Epub 2013 Mar 4"
COMMENT     Sequence Label: pRSET-RCaMP1h
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..43
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             47..79
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(83..91,92..106)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      113..122
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             134..190
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
ORIGIN
        1 agaaggagat atacatatgg gttctcatca tcatcatcat catggtatgg ctagcatgac
       61 tggtggacag caaatgggtc gggatctgta cgacgatgac gataaggatc tcgccaccat
      121 ggtcgactca tcacgtcgta agtggaataa gacaggtcac gcagtcagag ctataggtcg
      181 gctgagctca gcgatcaaca ccgaaatgat gtacccagcg gatggtggtc tgcgtggtta
      241 cactcacatg gcgctgaaag ttgatggcgg cggtcacctg tcctgttctt tcgtgaccac
      301 ctaccgctcc aaaaagactg tcggcaacat taagatgcct gccattcatt acgtcagcca
      361 ccgtctggag cgcctggagg agagcgataa cgaaatgttt gtcgtacagc gtgaacacgc
      421 agttgccaag tttgtgggcc tgggtggtgg cggcggtacc ggagggagca tgaactccct
      481 gatcaaggag aacatgcgta tgaaagtggt tctggaaggc tccgtaaacg gccaccagtt
      541 caaatgcact ggtgaaggcg aaggcaaccc gtatatgggc acccagacta tgcgtatcaa
      601 agtgatcgag ggtggtccgc tgccgtttgc gttcgacatc ctggcgacgt cctttatgta
      661 tggctcccgt accttcatca aatatccgaa aggcatcccg gatttcttta agcagtcctt
      721 cccggaaggt tt
//
