LOCUS       sequence-139950-        1006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 based UAS modified with a CCR5TC binding site upstream of it, 
            driving PSD95-FingR fused to GFP, CCR5TC zinc finger, and KRAB(A) 
            repressor domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1006)
  TITLE     Bonkowsky lab FingR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: zcUAS:PSD95.FingR-GFP-ZFC(CCR5TC)-KRAB(A)
FEATURES             Location/Qualifiers
     source          1..1006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     21..41
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        23..41
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     78..94
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     78..94
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(128..139,140..327)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    766..900
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(803..822)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     857..876
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     916..932
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    940..960
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcc
       61 accgcggtgg cggccgctct agaactagtg gatctgctgg gcttgctgaa ggtagggggt
      121 caagaaccag aggtgtaaag tacttgagta attttacttg attactgtac ttaagtatta
      181 tttttgggga tttttacttt acttgagtac aattaaaaat caatactttt acttttactt
      241 aattacattt ttttagaaaa aaaagtactt tttactcctt acaattttat ttacagtcaa
      301 aaagtactta ttttttggag atcacttcat tctattttcc cttgctatta ccaaaccaat
      361 tgaattgcgc tgatgcccag tttaatttaa atgttattta ttctgcctat gaaaatcgtt
      421 ttcacattat atgaaattgg tcagacatgt tcattggtcc tttggaagtg acgtcatgtc
      481 acatctatta ccacaatgca cagcaccttg acctggaaat tagggaaatt ataacagtca
      541 atcagtggaa gaaaatggag gaagtatgtg attcatcagc agctgcgagc agcacagtcc
      601 aaaatcagcc acaggatcaa gagcacccgt ggccgtatct tcgcagatct gatctagagg
      661 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
      721 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
      781 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      841 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
      901 atcatcgatg gtaccgtaaa acgacggcca gtgaattatc aactatgtat aataaagttg
      961 gaaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaa
//
