LOCUS       sequence-139954-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 based UAS modified with a IL2RGTC binding site upstream of it, 
            driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and 
            KRAB(A) repressor domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  TITLE     Bonkowsky lab FingR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-K...
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(399..423)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        423..441
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     478..494
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     478..494
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gggcggccct cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg
       61 tgcaccttga agcgcatgaa ctccttgatg atggccatgt tgtcctcctc gcccttgctc
      121 accatggatc cggtgcggta gttgatggag atcgggatcg ggtcctggta gttccagtgg
      181 tcgatcgtga cggcgtacac cgtgatggta tagtcgacac caggtttcag gctgctgatg
      241 gtagcagtgg acttgctgcc aggcacggtg aattcctgga cagggctatt gccaccagtt
      301 tcaccgtagg tgatgcggta gtagcgaacc atgaccttgt acttgcccca gctgatctgg
      361 atgctggttg gtgatgcttc cttgacttcg agcatggtag cctgcttttt tgtacaaact
      421 tgaattaacc ctcactaaag ggaacaaaag ctggagctcc accgcggtgg cggccgctct
      481 agaactagtg gatcccccgg gctgcaggaa ttcgtgtgga ggagctcaaa gtgaggctga
      541 gacgcgatgg gatccattat ataccctcta gagtcgaccc ttgccggagg acagtactcc
      601 gccagcccgg aggacagtac tccgccttgc gtcggaggac agtactccga cctctagtgt
      661 cggaggacag tactccgacc ttgccggagg acagtactcc gcagcccgga ggacagtact
      721 ccgccttgcg tcggaggaca gtactccgac ctctagtgtc ggaggacagt actccgacct
      781 tgccggagga cagtactccg ccagcccgga ggacagtact ccgccttgga ggcctaagct
      841 taccgtaaat agtccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
      901 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
      961 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     1021 attgattact attaataact agtactctgt g
//
