LOCUS       sequence-139955-        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 based UAS modified with a IL2RGTC binding site upstream of it, 
            driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and 
            KRAB(A) repressor domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Bonkowsky lab FingR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-K...
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     19..39
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        21..39
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     76..92
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     76..92
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(126..137,138..325)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    764..898
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(801..820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     855..874
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     914..930
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    938..958
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac
       61 cgcggtggcg gccgctctag aactagtgga tctgctgggc ttgctgaagg tagggggtca
      121 agaaccagag gtgtaaagta cttgagtaat tttacttgat tactgtactt aagtattatt
      181 tttggggatt tttactttac ttgagtacaa ttaaaaatca atacttttac ttttacttaa
      241 ttacattttt ttagaaaaaa aagtactttt tactccttac aattttattt acagtcaaaa
      301 agtacttatt ttttggagat cacttcattc tattttccct tgctattacc aaaccaattg
      361 aattgcgctg atgcccagtt taatttaaat gttatttatt ctgcctatga aaatcgtttt
      421 cacattatat gaaattggtc agacatgttc attggtcctt tggaagtgac gtcatgtcac
      481 atctattacc acaatgcaca gcaccttgac ctggaaatta gggaaattat aacagtcaat
      541 cagtggaaga aaatggagga agtatgtgat tcatcagcag ctgcgagcag cacagtccaa
      601 aatcagccac aggatcaaga gcacccgtgg ccgtatcttc gcagatctga tctagaggat
      661 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
      721 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
      781 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
      841 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcat
      901 catcgatggt accgtaaaac gacggccagt gaattatcaa ctatgtataa taaagttgga
      961 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     1021 aacttgttta ttgcagctta taatggttac aaataaagca atagc
//
