LOCUS       sequence-139971-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dCas9 expressed in pmax vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1445-pmax-dCas9
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..110
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(192..212)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagaccgagg agagggttag ggataggctt accttcgaac cgcgggccct ctagactcga
       61 gcggccgcca ctgtgctgga tatcaaccac tttgtacaag aaagctgggt cgaattctta
      121 attaatcagg ccggcccact accgccgccg ccggacccgc ctcctccgct accgccgcct
      181 ccgctggcct ccacctttct cttcttcttg gggctgtcgc ctcccagctg agacaggtcg
      241 atccgtgtct cgtacaggcc ggtgatgctc tggtggatca gggtggcgtc cagcacctct
      301 ttggtgctgg tgtacctctt ccggtcgatg gtggtgtcaa agtacttgaa ggcggcaggg
      361 gctcccagat tggtcagggt aaacaggtgg atgatattct cggcctgctc tctgatgggc
      421 ttatcccggt gcttgttgta ggcggacagc actttgtcca gattagcgtc ggccaggatc
      481 actctcttgg agaactcgct gatctgctcg atgatctcgt ccaggtagtg cttgtgctgt
      541 tccacaaaca gctgtttctg ctcattatcc tcgggggagc ccttcagctt ctcatagtgg
      601 ctggccaggt acaggaagtt cacatatttg gagggcaggg ccagttcgtt tcccttctgc
      661 agttcgccgg cagaggccag cattctcttc cggccgtttt ccagctcgaa cagggagtac
      721 ttaggcagct tgatgatcag gtcctttttc acttctttgt agcccttggc ttccagaaag
      781 tcgatgggat tcttctcgaa gctgcttctt tccatgatgg tgatccccag cagctctttc
      841 acactcttca gtttcttgga cttgcccttt tccactttgg ccaccaccag cacagaatag
      901 gccacggtgg ggctgtcgaa gccgccgtac ttcttagggt cccagtcctt ctttctggcg
      961 atcagcttat cgctgttcct cttgggcagg atagactctt tgctgaagcc gcctgtctgc
     1021 a
//
