LOCUS       sequence-139973-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BFPRKAB_PUFa in pCR8 gateway donor vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1450_pCR8-BFPKRAB_PUFa
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaattattt
       61 tttataatgc caactttgta caagaaagct gggtcgaatt cttaattaat caccctaagt
      121 caacaccgtt cttcatgtag tacttctcca gcttggccag aatgtgcttg ccataggtgt
      181 acttacgaag agttgcgatg tggggccgga tcttatgcat gacgatcttc cgctggcctg
      241 gctccgccac gtcaatcatc ttctggacca cgtagttggc atactggtcc ttcatcatgg
      301 tgtataaggc actgtgggga ccgtcgttca tggtgcacac ctcatcgatg agcacagcgc
      361 gctccgtacg tgaggcgtga gtaacacact tctccacaac attgcttgca aatttgtgct
      421 gactcaatac aagtacattg cctcggattt ctgctacaat tttgctttta tcctcaggac
      481 gaccgtgctc cagtacatgt tggattacat aatttccata ttgatcctgt accagctgct
      541 ctgtgtgctg gtgaagctcc tctaaaatag ggagtgtctg gtcagggaga cagtgctcca
      601 ggattctctg aatcactcgg cagccataag gatgtgtgga taaggcaaat acctgtccct
      661 taaacgcatc gatgataaat tgcaaagact ggggctgtac acattcaatg catttctgaa
      721 ccacgtgatt gccattctga tctttcacac acttcaagac atggccatct agttcccgaa
      781 ccatctcatt ctgctggtct gaaggaataa actcaagagc tttctcgata acacggctgc
      841 catacatctg tagtgccaat gacaggacgt ggcctcgaat ccgttctgcc aaagccagct
      901 tctgttcaag actgccaaat tcaaagaact tctgaatgac gtaattacca aacacatcca
      961 ccatgagttg gtaggcagcc tgg
//
