LOCUS       sequence-139974-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BFPRKAB_PUFa in pCR8 gateway donor vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1450_pCR8-BFPKRAB_PUFa
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             274..459
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             502..522
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat caagaccaca
       61 tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta tgtggactac
      121 agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca cgaggtggca
      181 gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatgg cggtggcgga
      241 gggatggatg ctaagtcact aactgcctgg tcccggacac tggtgacctt caaggatgta
      301 tttgtggact tcaccaggga ggagtggaag ctgctggaca ctgctcagca gatcgtgtac
      361 agaaatgtga tgctggagaa ctataagaac ctggtttcct tgggttatca gcttactaag
      421 ccagatgtga tcctccggtt ggagaaggga gaagagcccg gcggttccgg cggagggtcg
      481 gggccggccg ggatcctccc ccccaagaaa aagaggaagg tatctagagg ccgcagccgc
      541 cttttggaag attttcgaaa caaccggtac cccaatttac aactgcggga gattgctgga
      601 catataatgg aattttccca agaccagcat gggtccagat tcattcagct gaaactggag
      661 cgtgccacac cagctgagcg ccagcttgtc ttcaatgaaa tcctccaggc tgcctaccaa
      721 ctcatggtgg atgtgtttgg taattacgtc attcagaagt tctttgaatt tggcagtctt
      781 gaacagaagc tggctttggc agaacggatt cgaggccacg tcctgtcatt ggcactacag
      841 atgtatggca gccgtgttat cgagaaagct cttgagttta ttccttcaga ccagcagaat
      901 gagatggttc gggaactaga tggccatgtc ttgaagtgtg tgaaagatca gaatggcaat
      961 cacgtggttc a
//
