LOCUS       sequence-139985-        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  puc118 modified to contain ribosome binding site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Kappock
  TITLE     Vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK317
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    92..113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(128..133,134..151,152..158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    166..182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     171..193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     190..206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     190..206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(289..306)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(289..305)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(298..320)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      518..973
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(605..624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     815..836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(990..1009)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
       61 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
      121 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      181 aatttcacac aggaaacagc tatgacatga ttacgaattc aagaaggaga tatacatatg
      241 tatatctcct tcttgtctag agtcgacctg caggcatgca agcttggcac tggccgtcgt
      301 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      361 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      421 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      481 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
      541 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
      601 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
      661 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
      721 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
      781 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
      841 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
      901 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
      961 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     1021 cagccccgac
//
