LOCUS       sequence-139986-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK554
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..59)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(661..678)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(688..705)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    761..785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(785..804)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(786..804)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        850..927
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(947..966)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gctgcgctag tagacgagtc catgtgctgg cgttcaaatt tcgcagcagc ggtttcttta
       61 ccagactcga gtgcggccgc aagcttgtcg acggagctcg aattctcatt tatcttcggt
      121 aatgggagag ttggggacag aggccgtctg caatgcgcgc catgtttcaa ggcgtgcggc
      181 aagggccggg ttatacagtg caagaatgga ggcggccagc aaggcggcat ttttggcgcc
      241 ggatgcccca atagccaaag tgcccacagg cacgccgccg ggcatctgca caatggaaag
      301 caggctgtcc atgcctttaa gagcgcgaga ttctactgga acacccagca cgggcaggcg
      361 tgtccatgcc gcacacatac ccggcagatg tgcagctcca ccggccccag caataatcac
      421 attcaggccg cgttcggctg ccgtacgcgc ataatccgcc aaccggtctg gcgtacgatg
      481 tgctgaaaca atgagggttt catgcggaat ttccagctct gtcagcaatg cgtctgcatg
      541 gcgcatggtt tcccagtctg actggctgcc catgataatg cccacaacgg gggcagaggc
      601 tgctttatct tctaaagcac tggaagcaga gggcaggggg gcggtttcgc tcatcatatg
      661 gctgccgcgc ggcaccaggc cgctgctgtg atgatgatga tgatggctgc tgcccatggt
      721 atatctcctt attaaagtta aacaaaatta tttctacagg ggaattgtta tccgctcaca
      781 attcccctat agtgagtcgt attaatttcc taatgcagga gtcgcataag ggagagcgtc
      841 gagatcccgg acaccatcga atggcgcaaa acctttcgcg gtatggcatg atagcgcccg
      901 gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
      961 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgt
//
