LOCUS       sequence-139987-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human Plekhm1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Witwicka H, Jia H, Kutikov A, Reyes-Gutierrez P, Li X, Odgren PR
  TITLE     TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast 
            Acidification and Resorption.
  JOURNAL   PLoS One. 2015 May 19;10(5):e0127537. doi: 
            10.1371/journal.pone.0127537. eCollection 2015.
  PUBMED    25992615
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-19"; volume: "10(5):e0127537. doi"; pages: " 
            10.1371/journal.pone.0127537. eCollection 2015"
COMMENT     Sequence Label: hPlekhm1_pIRES puro Glue
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..219
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             238..258
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             265..291
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             265..291
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             292..369
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct tggtaccgag ctcggatcga tttaattaag ccaccatgga cgagaagacc
      121 accggctggc ggggcggcca cgtggtggag ggcctggccg gcgagctgga gcagctgcgg
      181 gcccggctgg agcaccaccc ccagggccag cgggagcccg gcggaagccc cggtggcgag
      241 aacctgtact tccagggcgc aagctacccc tacgacgtgc ccgactacgc caagcggcgg
      301 tggaagaaga acttcatcgc cgtgagcgcc gccaaccggt tcaagaagat cagcagcagc
      361 ggcgccctga ttgatggggg atctggcccc ggcggcggcg cgcctgtaca gatatcaatg
      421 ctttcagtgg tggagaatgg actggacccc caggctgcca tcccggtcat caagaagaag
      481 ctggtgggat ccgtgaaggc cttgcagaag cagtacgtgt ccctggacac ggtggtcact
      541 agtgaagacg gagatgccaa caccatgtgc agcgccctgg aggccgtatt tatccatggc
      601 ctgcacgcca agcacatccg agctgaggcc ggaggaaaaa ggaagaaaag tgcccaccag
      661 aagcctctgc cccagcctgt cttctggccc ctcctgaaag ctgtcaccca caaacacatc
      721 atctcagagt tggagcacct gacgtttgtc aacacggatg tgggccgctg ccgggcatgg
      781 ctgcggctgg ccctgaacga tggcctgatg gagtgctacc tgaagctgct gctgcaggag
      841 caggcccgct tgcatgagta ctaccagccc accgccctgc tccgggatgc tgagga
//
