LOCUS       sequence-139989-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 448 / 473, 
            Emission: 500 / 528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: miniSOG-ER-5
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..427
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
     polyA_signal    609..730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(646..665)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     700..719
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgcc accatgctgc tatccgtgcc
       61 gttgctgctc ggcctcctcg gcctggccgt cgccgcaccg gtcgccacca tggagaaaag
      121 tttcgtgata actgatccac ggctgccaga caatcccatc atcttcgcat ccgatggctt
      181 cctggagctg accgagtatt ccagagagga gatcctgggc cgcaatggcc gctttctgca
      241 gggaccagag acagaccagg ccacagtgca gaagattcgc gatgccatta gagatcagcg
      301 cgagattacc gtgcagctga taaactacac aaaaagcggg aagaaattct ggaacctcct
      361 gcacctccag cccatgaggg accagaaggg tgagctccag tatttcatcg gagtgcagct
      421 ggatggatcc ggaaaggacg agctgtaaga attctgcagt cgacggtacc gcgggcccgg
      481 gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac
      541 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
      601 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
      661 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
      721 atgtatctta acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
      781 tgttaaatca gctcattttt taaccaatag gccgaaatc
//
