LOCUS       sequence-139991-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This is hpRNA construct for gene silencing in soybean seeds. Soybean
            seed specific promoter, Glycinin gene promoter, is used..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Flores T, Karpova O, Su X, Zeng P, Bilyeu K, Sleper DA, Nguyen HT, 
            Zhang ZJ
  TITLE     Silencing of GmFAD3 gene by siRNA leads to low alpha-linolenic acids
            (18:3) of fad3-mutant phenotype in soybean [Glycine max (Merr.)].
  JOURNAL   Transgenic Res. 2008 Oct;17(5):839-50. doi: 
            10.1007/s11248-008-9167-6. Epub 2008 Feb 7.
  PUBMED    18256901
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s11248-008-9167-6"; journalName: "Transgenic Res"; date: 
            "2008-10"; volume: "17"; issue: "5"; pages: "839-50"
COMMENT     Sequence Label: pMU103
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    17..41
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    199..220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(235..240,241..258,259..265)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    273..289
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     278..300
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     297..313
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     297..313
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact
       61 gaaggcggga aacgacaatc tgatccaagc tcaagctcca atacgcaaac cgcctctccc
      121 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      181 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      241 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      301 aaacagctat gacatgatta cgaattctca acacaacata tacaaaacaa acgaatctca
      361 agcaatcaag cattctactt ctattgcagc aatttaaatc atttctttta aagcaaaagc
      421 aattttctga aaattttcac catttacgaa cgatagccat ggtaagtacg tactcaaaat
      481 gccaacaaat aaaaaaaaag ttgctttaat aatgccaaaa caaattaata aaacacttac
      541 aacaccggat tttttttaat taaaatgtgc catttaggat aaatagttaa tatttttaat
      601 aattatttaa aaagccgtat ctactaaaat gatttttatt tggttgaaaa tattaatatg
      661 tttaaatcaa cacaatctat caaaattaaa ctaaaaaaaa ataagtgtac gtggttaaca
      721 ttagtacagt aatataagag gaaaatgaga aattaagaaa ttgaaagcga gtctaatttt
      781 taaattatga acctgcatat ataa
//
