LOCUS       sequence-139996-         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human N-terminal part (M1-A497) Plekhm1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Witwicka H, Jia H, Kutikov A, Reyes-Gutierrez P, Li X, Odgren PR
  TITLE     TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast 
            Acidification and Resorption.
  JOURNAL   PLoS One. 2015 May 19;10(5):e0127537. doi: 
            10.1371/journal.pone.0127537. eCollection 2015.
  PUBMED    25992615
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-19"; volume: "10(5):e0127537. doi"; pages: " 
            10.1371/journal.pone.0127537. eCollection 2015"
COMMENT     Sequence Label: hPlekhm1-N/pIRES puro Glue (M1-A497)
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      101..110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             107..220
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             239..259
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             266..292
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             266..292
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             293..370
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 ggctaactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
       61 agacccaagc ttggtaccga gctcggatcg atttaattaa gccaccatgg acgagaagac
      121 caccggctgg cggggcggcc acgtggtgga gggcctggcc ggcgagctgg agcagctgcg
      181 ggcccggctg gagcaccacc cccagggcca gcgggagccc ggcggaagcc ccggtggcga
      241 gaacctgtac ttccagggcg caagctaccc ctacgacgtg cccgactacg ccaagcggcg
      301 gtggaagaag aacttcatcg ccgtgagcgc cgccaaccgg ttcaagaaga tcagcagcag
      361 cggcgccctg attgatgggg gatctggccc cggcggcggc gcgcctgtac agatatcaat
      421 gctttcagtg gtggagaatg gactggaccc ccaggctgcc atcccggtca tcaagaagaa
      481 gctggtggga tccgtgaagg ccttgcagaa gcagtacgtg tccctggaca cggtggtcac
      541 tagtgaagac ggagatgcca acaccatgtg cagcgccctg gaggccgtat ttatccatgg
      601 cctgcacgcc aagcacatcc gagctgaggc cggaggaaaa aggaagaaaa gtgcccacca
      661 gaagcctctg ccccagcctg tcttctggcc cctcctgaaa gctgtcaccc acaaacacat
      721 catctcagag ttggagcacc tgacgtttgt caacacggat gtgggccgct gccgggcatg
      781 gctgcggctg gccctgaacg atggcctgat ggagtgctac ctgaagctgc tgctgcagga
      841 gcaggcccgc ttgcatgagt actaccagcc caccgccctg ctccgggatg ctgag
//
