LOCUS       sequence-139997-        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap Single plasmid targeting vector for tagging Unc-32 with GFP 
            by CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS338
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(228..303)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(324..823)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans K09B11.12 U6 snRNA gene 
                     (Friedland et al., 2013)"
     protein_bind    926..947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(962..967,968..985,986..992)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1000..1016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1005..1027
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1024..1040
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1024..1040
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggcgtcgaga ttttgtaatt ttctttggaa tattgtcaaa aaaaatcaat tttttttcga
       61 gtttcctaat aaaatttttt ggaccaattt aatcaactct ggcaacttta aaatcgtccc
      121 tccgggaatt tacctttaac caagattagt tgaaacattg aactcaaaaa tttagagtat
      181 ttcattttag tttcaaaaag ggatatctag aatattattg aaaaaaagca ccgactcggt
      241 gccacttttt caagttgata acggactagc cttattttaa cttgctattt ctagctctaa
      301 aacaggtgat cttagagatt ctcaaacatt tagatttgca attcaattat atagggacca
      361 tttatcatgc agctgacata ttgagagtcc tgtaagacac tataccaaat ggggatgtag
      421 atcaataatt tgtgggggag gcatgtgtga ttcgtactcg ttcggataga gctttggtga
      481 atgtccaggc ttctgaaacg taggtgagcg ctggaaggac aattgagtcg aacagattcg
      541 cacgaatctt cttgtcggtg atggagtcgg tggcattctt gattccattg aatgcagccc
      601 aggctgctcg acgtcttcgg tggatttccg gcatcaagtt gttttgggcg ttgatttgac
      661 gaccgaggta gatgtactcg tcgacgtcgt cgagctgggt ggtgggggaa gggctaccga
      721 agtagacttt actggggtca gcgaatcggt ttcgcaagac tttcgtcttc ccagtattga
      781 tctcgagacc tacttcagag catttttgta cgagttcttg gagcccaata cgcaaaccgc
      841 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
      901 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
      961 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     1021 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaa
//
