LOCUS       sequence-140005-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Lee JY, Kwak JE, Moon J, Eom SH, Liong EC, Pedelacq JD, Berendzen J,
            Suh SW
  TITLE     Crystal structure and functional analysis of the SurE protein 
            identify a novel phosphatase family.
  JOURNAL   Nat Struct Biol. 2001 Sep . 8(9):789-94.
  PUBMED    11524683
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Biol. 2001 Sep . 8(9):789-94."
COMMENT     Sequence Label: pET-21a(+) SurE D9A
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             836..858
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    873..897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atcctcagtc gtacacctcc cttaacttct tcagacactg
      121 ttcgttcgtg agaaagggat gtatcggagt gatggagacg tacccttctc taaccgcctt
      181 gtagtcaaca tcgtctctat cgtcgtcttc gatgacctct cccatcatcc agtagtactt
      241 ctccccaaag ggtgagactc tctcttcgaa gtaatcgttc caccttcttc tgctctgcct
      301 tgtgaatctc catcccttta tctcaccagc aggcacgttt atgttcagca tggtgaaagg
      361 atcgaggagt gagaagtcga attctttcag aaagtcgatc aaaaaacgtg ccgctccttc
      421 gaaatcggga ctctcgtagt tagcactcga tatggcaatc gaaggaatgt tcatcatggc
      481 accttccatc gcacccgaga ccgtcccaga gtggagaata tccatgccca tgttgggccc
      541 cctgttcaca ccactcacga tgagatcgac ccttttatcc atgaccacgt tgtaggcgag
      601 tttcacacag tccgcgggcg ttcctgtagt ggagtacgca accaccctct ctgagatgaa
      661 aaccttcttc atccagagcg gaacgtgtat ggtgatgctg tgaccagttg cgctcctctc
      721 tttatcggga gccaccacga acacctcgtg ttcctcggaa agaagctctg cgaggacgat
      781 gatcccctta gaccgtatcc cggcgtcgtt ggttaccaga atacgcatat gtatatctcc
      841 ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca caattcc
//
