LOCUS       sequence-140012-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For CRISPR/Cas9 genome editing in Marchantia polymorpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, 
            Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T
  TITLE     Efficient CRISPR/Cas9-based genome editing and its application to 
            conditional genetic analysis in Marchantia polymorpha.
  JOURNAL   PLoS One. 2018 Oct 31;13(10):e0205117. doi: 
            10.1371/journal.pone.0205117. eCollection 2018.
  PUBMED    30379827
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-10-31"; volume: "13(10):e0205117. doi"; pages: " 
            10.1371/journal.pone.0205117. eCollection 2018"
COMMENT     Sequence Label: pMpGE010
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(52..176)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    205..229
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(732..752)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcataaagt gtaaagcctg gggtgcctaa tgcggcccct cgaggggccg ccatagtgac
       61 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
      121 atattgatat ttatatcatt ttacgtttct cgttcagctt ttttgtacaa acttgttgat
      181 aactctagtc aaccgctgtt atcaaccact ttgtacaaga aagctgggtc ggcgcgccca
      241 cccttaagcc tatactgtac ttaacttgat tgcataatta cttgatcata gactcatagt
      301 aaacttgatt acacagataa gtgaagaaac aaaccaattc aagacataac caaagagagg
      361 tgaaagactg ttttatatgt ctaacattgc accttaatat cacactgtta gttcctttct
      421 tacttaaatt caacccatta aagtaaaaac aacagataat aataatttga gaatgaacaa
      481 aaggaccata tcatttatta actcttatcc atccatttgc attttgatgt ccgaaaacaa
      541 aaactgaaag aacacagtaa attacaagca gaacaaatga tagaagaaaa cagcttttcc
      601 aatgccataa tactcaaact tagtaggatt ctggtgtgtg ggcaatgaaa ctgatgcatt
      661 gaacttgacg aacgttgtcg aaaccgatga tacggacgaa agctgggagg cctggagctc
      721 ggcgcgcctc aaaccttcct cttcttctta ggatcagccc ttgaatcacc accgagctgt
      781 gagagatcga tcctagtctc gtagagtcca gtgatagact gatggatgag g
//
