LOCUS       sequence-140016-       17689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For CRISPR/Cas9 genome editing in Marchantia polymorpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17689)
  AUTHORS   Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, 
            Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T
  TITLE     Efficient CRISPR/Cas9-based genome editing and its application to 
            conditional genetic analysis in Marchantia polymorpha.
  JOURNAL   PLoS One. 2018 Oct 31;13(10):e0205117. doi: 
            10.1371/journal.pone.0205117. eCollection 2018.
  PUBMED    30379827
REFERENCE   2  (bases 1 to 17689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-10-31"; volume: "13(10):e0205117. doi"; pages: " 
            10.1371/journal.pone.0205117. eCollection 2018"
COMMENT     Sequence Label: pMpGE010
FEATURES             Location/Qualifiers
     source          1..17689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2081
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2102..6205
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2102..6205
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6218..6238
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(6741..6765)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    6794..6918
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6955..6960,6961..6978,6979..6985)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7039..7698
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7105..7124)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8040..8345
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8224..8243
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8386..8510)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      8550..8802
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(8835..8851)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8835..8851)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8848..8870)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8859..8875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8883..8889,8890..8907,8908..8913))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8928..8949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    9034..9208
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9249..10274)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10341..11016)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    11211..11235
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11756..12547
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      12793..13381
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13282..13301
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13535..13552
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    13567..13707
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(13633..13652)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13793..13815
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14051..14245
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14311..15384)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(15813..16442)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagcaggc tccgcggccg cccccttcac
     2101 catggataag aagtactcta tcggactcga tatcggaact aactctgtgg gatgggctgt
     2161 gatcaccgat gagtacaagg tgccatctaa gaagttcaag gttctcggaa acaccgatag
     2221 gcactctatc aagaaaaacc ttatcggtgc tctcctcttc gattctggtg aaactgctga
     2281 ggctaccaga ctcaagagaa ccgctagaag aaggtacacc agaagaaaga acaggatctg
     2341 ctacctccaa gagatcttct ctaacgagat ggctaaagtg gatgattcat tcttccacag
     2401 gctcgaagag tcattcctcg tggaagaaga taagaagcac gagaggcacc ctatcttcgg
     2461 aaacatcgtt gatgaggtgg cataccacga gaagtaccct actatctacc acctcagaaa
     2521 gaagctcgtt gattctactg ataaggctga tctcaggctc atctacctcg ctctcgctca
     2581 catgatcaag ttcagaggac acttcctcat cgagggtgat ctcaaccctg ataactctga
     2641 tgtggataag ttgttcatcc agctcgtgca gacctacaac cagcttttcg aagagaaccc
     2701 tatcaacgct tcaggtgtgg atgctaaggc tatcctctct gctaggctct ctaagtcaag
     2761 aaggcttgag aacctcattg ctcagctccc tggtgagaag aagaacggac ttttcggaaa
     2821 cttgatcgct ctctctctcg gactcacccc taacttcaag tctaacttcg atctcgctga
     2881 ggatgcaaag ctccagctct caaaggatac ctacgatgat gatctcgata acctcctcgc
     2941 tcagatcgga gatcagtacg ctgatttgtt cctcgctgct aagaacctct ctgatgctat
     3001 cctcctcagt gatatcctca gagtgaacac cgagatcacc aaggctccac tctcagcttc
     3061 tatgatcaag agatacgatg agcaccacca ggatctcaca cttctcaagg ctcttgttag
     3121 acagcagctc ccagagaagt acaaagagat tttcttcgat cagtctaaga acggatacgc
     3181 tggttacatc gatggtggtg catctcaaga agagttctac aagttcatca agcctatcct
     3241 cgagaagatg gatggaaccg aggaactcct cgtgaagctc aatagagagg atcttctcag
     3301 aaagcagagg accttcgata acggatctat ccctcatcag atccacctcg gagagttgca
     3361 cgctatcctt agaaggcaag aggatttcta cccattcctc aaggataaca gggaaaagat
     3421 tgagaagatt ctcaccttca gaatccctta ctacgtggga cctctcgcta gaggaaactc
     3481 aagattcgct tggatgacca gaaagtctga ggaaaccatc accccttgga acttcgaaga
     3541 ggtggtggat aagggtgcta gtgctcagtc tttcatcgag aggatgacca acttcgataa
     3601 gaaccttcca aacgagaagg tgctccctaa gcactctttg ctctacgagt acttcaccgt
     3661 gtacaacgag ttgaccaagg ttaagtacgt gaccgaggga atgaggaagc ctgctttttt
     3721 gtcaggtgag caaaagaagg ctatcgttga tctcttgttc aagaccaaca gaaaggtgac
     3781 cgtgaagcag ctcaaagagg attacttcaa gaaaatcgag tgcttcgatt cagttgagat
     3841 ttctggtgtt gaggataggt tcaacgcatc tctcggaacc taccacgatc tcctcaagat
     3901 cattaaggat aaggatttct tggataacga ggaaaacgag gatatcttgg aggatatcgt
     3961 tcttaccctc accctctttg aagatagaga gatgattgaa gaaaggctca agacctacgc
     4021 tcatctcttc gatgataagg tgatgaagca gttgaagaga agaagataca ctggttgggg
     4081 aaggctctca agaaagctca ttaacggaat cagggataag cagtctggaa agacaatcct
     4141 tgatttcctc aagtctgatg gattcgctaa cagaaacttc atgcagctca tccacgatga
     4201 ttctctcacc tttaaagagg atatccagaa ggctcaggtt tcaggacagg gtgatagtct
     4261 ccatgagcat atcgctaacc tcgctggatc tcctgcaatc aagaagggaa tcctccagac
     4321 tgtgaaggtt gtggatgagt tggtgaaggt gatgggaagg cataagcctg agaacatcgt
     4381 gatcgaaatg gctagagaga accagaccac tcagaaggga cagaagaact ctagggaaag
     4441 gatgaagagg atcgaggaag gtatcaaaga gcttggatct cagatcctca aagagcaccc
     4501 tgttgagaac actcagctcc agaatgagaa gctctacctc tactacctcc agaacggaag
     4561 ggatatgtat gtggatcaag agttggatat caacaggctc tctgattacg atgttgatca
     4621 tatcgtgcca cagtcattct tgaaggatga ttctatcgat aacaaggtgc tcaccaggtc
     4681 tgataagaac aggggtaaga gtgataacgt gccaagtgaa gaggttgtga agaaaatgaa
     4741 gaactattgg aggcagctcc tcaacgctaa gctcatcact cagagaaagt tcgataactt
     4801 gactaaggct gagaggggag gactctctga attggataag gcaggattca tcaagaggca
     4861 gcttgtggaa accaggcaga tcactaagca cgttgcacag atcctcgatt ctaggatgaa
     4921 caccaagtac gatgagaacg ataagttgat cagggaagtg aaggttatca ccctcaagtc
     4981 aaagctcgtg tctgatttca gaaaggattt ccaattctac aaggtgaggg aaatcaacaa
     5041 ctaccaccac gctcacgatg cttaccttaa cgctgttgtt ggaaccgctc tcatcaagaa
     5101 gtatcctaag ctcgagtcag agttcgtgta cggtgattac aaggtgtacg atgtgaggaa
     5161 gatgatcgct aagtctgagc aagagatcgg aaaggctacc gctaagtatt tcttctactc
     5221 taacatcatg aatttcttca agaccgagat taccctcgct aacggtgaga tcagaaagag
     5281 gccactcatc gagacaaacg gtgaaacagg tgagatcgtg tgggataagg gaagggattt
     5341 cgctaccgtt agaaaggtgc tctctatgcc acaggtgaac atcgttaaga aaaccgaggt
     5401 gcagaccggt ggattctcta aagagtctat cctccctaag aggaactctg ataagctcat
     5461 tgctaggaag aaggattggg accctaagaa atacggtggt ttcgattctc ctaccgtggc
     5521 ttactctgtt ctcgttgtgg ctaaggttga gaagggaaag agtaagaagc tcaagtctgt
     5581 taaggaactt ctcggaatca ctatcatgga aaggtcatct ttcgagaaga acccaatcga
     5641 tttcctcgag gctaagggat acaaagaggt taagaaggat ctcatcatca agctcccaaa
     5701 gtactcactc ttcgaactcg agaacggtag aaagaggatg ctcgcttctg ctggtgagct
     5761 tcaaaaggga aacgagcttg ctctcccatc taagtacgtt aactttcttt acctcgcttc
     5821 tcactacgag aagttgaagg gatctccaga agataacgag cagaagcaac ttttcgttga
     5881 gcagcacaag cactacttgg atgagatcat cgagcagatc tctgagttct ctaaaagggt
     5941 gatcctcgct gatgcaaacc tcgataaggt gttgtctgct tacaacaagc acagagataa
     6001 gcctatcagg gaacaggcag agaacatcat ccatctcttc acccttacca acctcggtgc
     6061 tcctgctgct ttcaagtact tcgatacaac catcgatagg aagagataca cctctaccaa
     6121 agaagtgctc gatgctaccc tcatccatca gtctatcact ggactctacg agactaggat
     6181 cgatctctca cagctcggtg gtgattcaag ggctgatcct aagaagaaga ggaaggtttg
     6241 aggcgcgccg agctccaggc ctcccagctt tcgtccgtat catcggtttc gacaacgttc
     6301 gtcaagttca atgcatcagt ttcattgccc acacaccaga atcctactaa gtttgagtat
     6361 tatggcattg gaaaagctgt tttcttctat catttgttct gcttgtaatt tactgtgttc
     6421 tttcagtttt tgttttcgga catcaaaatg caaatggatg gataagagtt aataaatgat
     6481 atggtccttt tgttcattct caaattatta ttatctgttg tttttacttt aatgggttga
     6541 atttaagtaa gaaaggaact aacagtgtga tattaaggtg caatgttaga catataaaac
     6601 agtctttcac ctctctttgg ttatgtcttg aattggtttg tttcttcact tatctgtgta
     6661 atcaagttta ctatgagtct atgatcaagt aattatgcaa tcaagttaag tacagtatag
     6721 gcttaagggt gggcgcgccg acccagcttt cttgtacaaa gtggttgata acagcggttg
     6781 actagagtta tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     6841 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     6901 acatatccag tcactatggc ggcccctcga ggggccgcat taggcacccc aggctttaca
     6961 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccggc gagattttca
     7021 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     7081 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     7141 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     7201 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     7261 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     7321 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     7381 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     7441 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     7501 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     7561 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     7621 gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     7681 tggcagggcg gggcgtaaag atctggatcc ggcttactaa aagccagata acagtatgcg
     7741 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     7801 tcaaaaagag gtgtgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     7861 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     7921 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     7981 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg actggtgaaa
     8041 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     8101 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     8161 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     8221 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     8281 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     8341 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     8401 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     8461 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt tgataacagc
     8521 gcttagaggc ttagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct
     8581 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     8641 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     8701 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     8761 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg gttccggaac
     8821 caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8881 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8941 ctcacattag gctgaattag gcgcgcctat ttctgaattc aacacattgc ggacgttttt
     9001 aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac tggattttgg
     9061 ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata catactaagg
     9121 gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc ccttatctgg
     9181 gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga tcccggtcgg
     9241 catctactct atttctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg
     9301 agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc
     9361 ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca
     9421 tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata
     9481 tacgcccgga gtcgtggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc
     9541 tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg
     9601 gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc
     9661 aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg
     9721 gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc
     9781 acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta
     9841 gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg
     9901 gccgcagcga tcgcatccat agcctccgcg accggttgta gaacagcggg cagttcggtt
     9961 tcaggcaggt cttgcaacgt gacaccctgt gaacggcggg agatgcaata ggtcaggctc
    10021 tcgctaaact ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc
    10081 cgataaacat aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat
    10141 ccacgccctc ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc
    10201 aggtcggaga cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt
    10261 tcaggctttt tcatatctca ttgccccccg gatctgcgaa agctcgagag agatagattt
    10321 gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact tccttatata
    10381 gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat
    10441 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
    10501 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
    10561 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
    10621 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
    10681 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
    10741 cgagagtgtc gtgctccacc atgttcacat caatccactt gctttgaaga cgtggttgga
    10801 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
    10861 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
    10921 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
    10981 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
    11041 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt ggcaagctgc
    11101 tctagccaat acgcaaaccg aattcagaaa taaattcagc ctaattcggc gttaattcag
    11161 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
    11221 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
    11281 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
    11341 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
    11401 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
    11461 ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
    11521 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
    11581 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
    11641 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
    11701 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg
    11761 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
    11821 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
    11881 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
    11941 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
    12001 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
    12061 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
    12121 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
    12181 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
    12241 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
    12301 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
    12361 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
    12421 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
    12481 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
    12541 caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact
    12601 caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc
    12661 ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat
    12721 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    12781 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    12841 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    12901 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
    12961 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    13021 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    13081 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    13141 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    13201 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    13261 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    13321 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    13381 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    13441 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    13501 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    13561 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
    13621 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
    13681 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
    13741 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
    13801 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg
    13861 tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
    13921 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
    13981 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
    14041 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
    14101 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
    14161 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
    14221 gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag
    14281 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
    14341 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
    14401 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
    14461 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
    14521 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
    14581 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
    14641 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
    14701 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
    14761 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
    14821 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
    14881 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
    14941 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
    15001 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
    15061 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
    15121 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
    15181 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
    15241 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
    15301 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
    15361 gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg
    15421 cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
    15481 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
    15541 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
    15601 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
    15661 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
    15721 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
    15781 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
    15841 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
    15901 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
    15961 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
    16021 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
    16081 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
    16141 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
    16201 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
    16261 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
    16321 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
    16381 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
    16441 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
    16501 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
    16561 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
    16621 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
    16681 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
    16741 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
    16801 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
    16861 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
    16921 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
    16981 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
    17041 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
    17101 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
    17161 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
    17221 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
    17281 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
    17341 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
    17401 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
    17461 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
    17521 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
    17581 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
    17641 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
