LOCUS       sequence-140017-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human C-terminal part (S778-Q986) Plekhm1 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Witwicka H, Jia H, Kutikov A, Reyes-Gutierrez P, Li X, Odgren PR
  TITLE     TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast 
            Acidification and Resorption.
  JOURNAL   PLoS One. 2015 May 19;10(5):e0127537. doi: 
            10.1371/journal.pone.0127537. eCollection 2015.
  PUBMED    25992615
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-19"; volume: "10(5):e0127537. doi"; pages: " 
            10.1371/journal.pone.0127537. eCollection 2015"
COMMENT     Sequence Label: hPlekhm1-C/pIRES puro Glue (S778-Q986)
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(731..808)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             complement(809..835)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(809..835)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(842..862)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ggcggccgtt actagtggat ccgaaaaggg cgaattccgt cgacgcggcc gcttactggg
       61 aggcaaattc aatcagggcc ttgaggaatc cttcatacac cccgtctgcg atctgttgga
      121 ggtcagcaac actgaaccta tgcggagatt ccaagagata attcctgtgg ttgagcctct
      181 tgctgagctc cttcagggcg ccactccggc acaggcccag gtaatccccc aggagcttca
      241 gctgctcccg tctcctccca atgaggtgca tccgctccac atgctcgtac agagacgcgt
      301 tcaccatctg caggttgatg aggggctggg cccggatctg tgtcagaaac ttcagggcct
      361 gcctgcagat cgggcgcttg gtgaggtccc agttgtggat gatcctggcc ggaatcactg
      421 aggcatcgtc ttggtggcag atgtcacagt aatagaggcc agagaaggca cagagcttgg
      481 gtcgtacaaa ggagaagccg atctgccggg agcagcctgc gcagaagcag ccttgggagt
      541 caaggccttt ctccatgggg atagccacca ggtactgcag caggaagcca ttctcccggg
      601 tggcaaattt cagcacctcc tgacagtttt catccaggct cccgcccagg gtcaccgcct
      661 cctcggctgt ctccaagtag gaggatatct gtacaggcgc gccgccgccg gggccagatc
      721 ccccatcaat cagggcgccg ctgctgctga tcttcttgaa ccggttggcg gcgctcacgg
      781 cgatgaagtt cttcttccac cgccgcttgg cgtagtcggg cacgtcgtag gggtagcttg
      841 cgccctggaa gtacaggttc tcgccaccgg ggcttccgcc gggctcccgc tggccctggg
      901 ggtggtgctc cagccgggcc cgcagctgct ccagctcgcc ggccaggccc t
//
