LOCUS       sequence-140019-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human C-terminal part (H987-end) Plekhm1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Witwicka H, Jia H, Kutikov A, Reyes-Gutierrez P, Li X, Odgren PR
  TITLE     TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast 
            Acidification and Resorption.
  JOURNAL   PLoS One. 2015 May 19;10(5):e0127537. doi: 
            10.1371/journal.pone.0127537. eCollection 2015.
  PUBMED    25992615
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-19"; volume: "10(5):e0127537. doi"; pages: " 
            10.1371/journal.pone.0127537. eCollection 2015"
COMMENT     Sequence Label: hPlekhm1-C/pIRES puro Glue (H987-end)
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     39..58
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        39..57
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..218
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             237..257
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             264..290
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             264..290
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             291..368
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
       61 acccaagctt ggtaccgagc tcggatcgat ttaattaagc caccatggac gagaagacca
      121 ccggctggcg gggcggccac gtggtggagg gcctggccgg cgagctggag cagctgcggg
      181 cccggctgga gcaccacccc cagggccagc gggagcccgg cggaagcccc ggtggcgaga
      241 acctgtactt ccagggcgca agctacccct acgacgtgcc cgactacgcc aagcggcggt
      301 ggaagaagaa cttcatcgcc gtgagcgccg ccaaccggtt caagaagatc agcagcagcg
      361 gcgccctgat tgatggggga tctggccccg gcggcggcgc gcctgtacag atatcccatg
      421 tctaccactg cgacctgtgc acccagcgcg gcttcatctg ccagatctgc cagcaccacg
      481 acatcatctt cccctttgag tttgacacca cagtcaggtg tgccgagtgc aagaccgtct
      541 tccaccagag ctgccaggct gtggtgaaga agggctgccc ccgctgtgcc cgccggcgca
      601 agtaccagga acagaacatt ttcgcctgag cggccgcgtc gacggaattc gcccttttcg
      661 gatccactag taacggccgc cagtgtgctg gaattaattc gctgtctgcg agggccagct
      721 gttggggtga gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt
      781 ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg agggtggccg
      841 cgtccatctg gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg
      901 a
//
