LOCUS       sequence-140020-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For CRISPR/Cas9 genome editing in Marchantia polymorpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, 
            Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T
  TITLE     Efficient CRISPR/Cas9-based genome editing and its application to 
            conditional genetic analysis in Marchantia polymorpha.
  JOURNAL   PLoS One. 2018 Oct 31;13(10):e0205117. doi: 
            10.1371/journal.pone.0205117. eCollection 2018.
  PUBMED    30379827
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-10-31"; volume: "13(10):e0205117. doi"; pages: " 
            10.1371/journal.pone.0205117. eCollection 2018"
COMMENT     Sequence Label: pMpGE011
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(51..175)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    204..228
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(731..751)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gcataaagtg taaagcctgg ggtgcctaat gcggcccctc gaggggccgc catagtgact
       61 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
      121 tattgatatt tatatcattt tacgtttctc gttcagcttt tttgtacaaa cttgttgata
      181 actctagtca accgctgtta tcaaccactt tgtacaagaa agctgggtcg gcgcgcccac
      241 ccttaagcct atactgtact taacttgatt gcataattac ttgatcatag actcatagta
      301 aacttgatta cacagataag tgaagaaaca aaccaattca agacataacc aaagagaggt
      361 gaaagactgt tttatatgtc taacattgca ccttaatatc acactgttag ttcctttctt
      421 acttaaattc aacccattaa agtaaaaaca acagataata ataatttgag aatgaacaaa
      481 aggaccatat catttattaa ctcttatcca tccatttgca ttttgatgtc cgaaaacaaa
      541 aactgaaaga acacagtaaa ttacaagcag aacaaatgat agaagaaaac agcttttcca
      601 atgccataat actcaaactt agtaggattc tggtgtgtgg gcaatgaaac tgatgcattg
      661 aacttgacga acgttgtcga aaccgatgat acggacgaaa gctgggaggc ctggagctcg
      721 gcgcgcctca aaccttcctc ttcttcttag gatcagccct tgaatcacca ccgagctgtg
      781 agagatcgat cctagtctcg t
//
