LOCUS       sequence-140021-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human C-terminal part (H987-end) Plekhm1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Witwicka H, Jia H, Kutikov A, Reyes-Gutierrez P, Li X, Odgren PR
  TITLE     TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast 
            Acidification and Resorption.
  JOURNAL   PLoS One. 2015 May 19;10(5):e0127537. doi: 
            10.1371/journal.pone.0127537. eCollection 2015.
  PUBMED    25992615
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-19"; volume: "10(5):e0127537. doi"; pages: " 
            10.1371/journal.pone.0127537. eCollection 2015"
COMMENT     Sequence Label: hPlekhm1-C/pIRES puro Glue (H987-end)
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(313..390)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             complement(391..417)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(391..417)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(424..444)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(463..576)
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     regulatory      573..582
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(623..642)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(624..642)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(687..890)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(716..736)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gcggccgtta ctagtggatc cgaaaagggc gaattccgtc gacgcggccg ctcaggcgaa
       61 aatgttctgt tcctggtact tgcgccggcg ggcacagcgg gggcagccct tcttcaccac
      121 agcctggcag ctctggtgga agacggtctt gcactcggca cacctgactg tggtgtcaaa
      181 ctcaaagggg aagatgatgt cgtggtgctg gcagatctgg cagatgaagc cgcgctgggt
      241 gcacaggtcg cagtggtaga catgggatat ctgtacaggc gcgccgccgc cggggccaga
      301 tcccccatca atcagggcgc cgctgctgct gatcttcttg aaccggttgg cggcgctcac
      361 ggcgatgaag ttcttcttcc accgccgctt ggcgtagtcg ggcacgtcgt aggggtagct
      421 tgcgccctgg aagtacaggt tctcgccacc ggggcttccg ccgggctccc gctggccctg
      481 ggggtggtgc tccagccggg cccgcagctg ctccagctcg ccggccaggc cctccaccac
      541 gtggccgccc cgccagccgg tggtcttctc gtccatggtg gcttaattaa atcgatccga
      601 gctcggtacc aagcttgggt ctccctatag tgagtcgtat taatttcgat aagccagtaa
      661 gcagtgggtt ctctagttag ccagagagct ctgcttatat agacctccca ccgtacacgc
      721 ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga
      781 ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
      841 gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata
      901 gcgatgacta atacgta
//
