LOCUS       sequence-140022-       18702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For CRISPR/Cas9 genome editing in Marchantia polymorpha.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18702)
  AUTHORS   Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, 
            Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T
  TITLE     Efficient CRISPR/Cas9-based genome editing and its application to 
            conditional genetic analysis in Marchantia polymorpha.
  JOURNAL   PLoS One. 2018 Oct 31;13(10):e0205117. doi: 
            10.1371/journal.pone.0205117. eCollection 2018.
  PUBMED    30379827
REFERENCE   2  (bases 1 to 18702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-10-31"; volume: "13(10):e0205117. doi"; pages: " 
            10.1371/journal.pone.0205117. eCollection 2018"
COMMENT     Sequence Label: pMpGE011
FEATURES             Location/Qualifiers
     source          1..18702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2081
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2102..6205
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2102..6205
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6218..6238
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(6741..6765)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    6794..6918
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6955..6960,6961..6978,6979..6985)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             7039..7698
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7105..7124)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             8040..8345
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8224..8243
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8386..8510)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(8837..8853)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8837..8853)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8850..8872)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8861..8877
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8885..8891,8892..8909,8910..8915))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8930..8951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    9036..9210
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(11354..12029)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    12224..12248
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12769..13560
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      13806..14394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14295..14314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14548..14565
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14580..14720
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(14646..14665)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14806..14828
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15064..15258
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(15324..16397)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16826..17455)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagcaggc tccgcggccg cccccttcac
     2101 catggataag aagtactcta tcggactcga tatcggaact aactctgtgg gatgggctgt
     2161 gatcaccgat gagtacaagg tgccatctaa gaagttcaag gttctcggaa acaccgatag
     2221 gcactctatc aagaaaaacc ttatcggtgc tctcctcttc gattctggtg aaactgctga
     2281 ggctaccaga ctcaagagaa ccgctagaag aaggtacacc agaagaaaga acaggatctg
     2341 ctacctccaa gagatcttct ctaacgagat ggctaaagtg gatgattcat tcttccacag
     2401 gctcgaagag tcattcctcg tggaagaaga taagaagcac gagaggcacc ctatcttcgg
     2461 aaacatcgtt gatgaggtgg cataccacga gaagtaccct actatctacc acctcagaaa
     2521 gaagctcgtt gattctactg ataaggctga tctcaggctc atctacctcg ctctcgctca
     2581 catgatcaag ttcagaggac acttcctcat cgagggtgat ctcaaccctg ataactctga
     2641 tgtggataag ttgttcatcc agctcgtgca gacctacaac cagcttttcg aagagaaccc
     2701 tatcaacgct tcaggtgtgg atgctaaggc tatcctctct gctaggctct ctaagtcaag
     2761 aaggcttgag aacctcattg ctcagctccc tggtgagaag aagaacggac ttttcggaaa
     2821 cttgatcgct ctctctctcg gactcacccc taacttcaag tctaacttcg atctcgctga
     2881 ggatgcaaag ctccagctct caaaggatac ctacgatgat gatctcgata acctcctcgc
     2941 tcagatcgga gatcagtacg ctgatttgtt cctcgctgct aagaacctct ctgatgctat
     3001 cctcctcagt gatatcctca gagtgaacac cgagatcacc aaggctccac tctcagcttc
     3061 tatgatcaag agatacgatg agcaccacca ggatctcaca cttctcaagg ctcttgttag
     3121 acagcagctc ccagagaagt acaaagagat tttcttcgat cagtctaaga acggatacgc
     3181 tggttacatc gatggtggtg catctcaaga agagttctac aagttcatca agcctatcct
     3241 cgagaagatg gatggaaccg aggaactcct cgtgaagctc aatagagagg atcttctcag
     3301 aaagcagagg accttcgata acggatctat ccctcatcag atccacctcg gagagttgca
     3361 cgctatcctt agaaggcaag aggatttcta cccattcctc aaggataaca gggaaaagat
     3421 tgagaagatt ctcaccttca gaatccctta ctacgtggga cctctcgcta gaggaaactc
     3481 aagattcgct tggatgacca gaaagtctga ggaaaccatc accccttgga acttcgaaga
     3541 ggtggtggat aagggtgcta gtgctcagtc tttcatcgag aggatgacca acttcgataa
     3601 gaaccttcca aacgagaagg tgctccctaa gcactctttg ctctacgagt acttcaccgt
     3661 gtacaacgag ttgaccaagg ttaagtacgt gaccgaggga atgaggaagc ctgctttttt
     3721 gtcaggtgag caaaagaagg ctatcgttga tctcttgttc aagaccaaca gaaaggtgac
     3781 cgtgaagcag ctcaaagagg attacttcaa gaaaatcgag tgcttcgatt cagttgagat
     3841 ttctggtgtt gaggataggt tcaacgcatc tctcggaacc taccacgatc tcctcaagat
     3901 cattaaggat aaggatttct tggataacga ggaaaacgag gatatcttgg aggatatcgt
     3961 tcttaccctc accctctttg aagatagaga gatgattgaa gaaaggctca agacctacgc
     4021 tcatctcttc gatgataagg tgatgaagca gttgaagaga agaagataca ctggttgggg
     4081 aaggctctca agaaagctca ttaacggaat cagggataag cagtctggaa agacaatcct
     4141 tgatttcctc aagtctgatg gattcgctaa cagaaacttc atgcagctca tccacgatga
     4201 ttctctcacc tttaaagagg atatccagaa ggctcaggtt tcaggacagg gtgatagtct
     4261 ccatgagcat atcgctaacc tcgctggatc tcctgcaatc aagaagggaa tcctccagac
     4321 tgtgaaggtt gtggatgagt tggtgaaggt gatgggaagg cataagcctg agaacatcgt
     4381 gatcgaaatg gctagagaga accagaccac tcagaaggga cagaagaact ctagggaaag
     4441 gatgaagagg atcgaggaag gtatcaaaga gcttggatct cagatcctca aagagcaccc
     4501 tgttgagaac actcagctcc agaatgagaa gctctacctc tactacctcc agaacggaag
     4561 ggatatgtat gtggatcaag agttggatat caacaggctc tctgattacg atgttgatca
     4621 tatcgtgcca cagtcattct tgaaggatga ttctatcgat aacaaggtgc tcaccaggtc
     4681 tgataagaac aggggtaaga gtgataacgt gccaagtgaa gaggttgtga agaaaatgaa
     4741 gaactattgg aggcagctcc tcaacgctaa gctcatcact cagagaaagt tcgataactt
     4801 gactaaggct gagaggggag gactctctga attggataag gcaggattca tcaagaggca
     4861 gcttgtggaa accaggcaga tcactaagca cgttgcacag atcctcgatt ctaggatgaa
     4921 caccaagtac gatgagaacg ataagttgat cagggaagtg aaggttatca ccctcaagtc
     4981 aaagctcgtg tctgatttca gaaaggattt ccaattctac aaggtgaggg aaatcaacaa
     5041 ctaccaccac gctcacgatg cttaccttaa cgctgttgtt ggaaccgctc tcatcaagaa
     5101 gtatcctaag ctcgagtcag agttcgtgta cggtgattac aaggtgtacg atgtgaggaa
     5161 gatgatcgct aagtctgagc aagagatcgg aaaggctacc gctaagtatt tcttctactc
     5221 taacatcatg aatttcttca agaccgagat taccctcgct aacggtgaga tcagaaagag
     5281 gccactcatc gagacaaacg gtgaaacagg tgagatcgtg tgggataagg gaagggattt
     5341 cgctaccgtt agaaaggtgc tctctatgcc acaggtgaac atcgttaaga aaaccgaggt
     5401 gcagaccggt ggattctcta aagagtctat cctccctaag aggaactctg ataagctcat
     5461 tgctaggaag aaggattggg accctaagaa atacggtggt ttcgattctc ctaccgtggc
     5521 ttactctgtt ctcgttgtgg ctaaggttga gaagggaaag agtaagaagc tcaagtctgt
     5581 taaggaactt ctcggaatca ctatcatgga aaggtcatct ttcgagaaga acccaatcga
     5641 tttcctcgag gctaagggat acaaagaggt taagaaggat ctcatcatca agctcccaaa
     5701 gtactcactc ttcgaactcg agaacggtag aaagaggatg ctcgcttctg ctggtgagct
     5761 tcaaaaggga aacgagcttg ctctcccatc taagtacgtt aactttcttt acctcgcttc
     5821 tcactacgag aagttgaagg gatctccaga agataacgag cagaagcaac ttttcgttga
     5881 gcagcacaag cactacttgg atgagatcat cgagcagatc tctgagttct ctaaaagggt
     5941 gatcctcgct gatgcaaacc tcgataaggt gttgtctgct tacaacaagc acagagataa
     6001 gcctatcagg gaacaggcag agaacatcat ccatctcttc acccttacca acctcggtgc
     6061 tcctgctgct ttcaagtact tcgatacaac catcgatagg aagagataca cctctaccaa
     6121 agaagtgctc gatgctaccc tcatccatca gtctatcact ggactctacg agactaggat
     6181 cgatctctca cagctcggtg gtgattcaag ggctgatcct aagaagaaga ggaaggtttg
     6241 aggcgcgccg agctccaggc ctcccagctt tcgtccgtat catcggtttc gacaacgttc
     6301 gtcaagttca atgcatcagt ttcattgccc acacaccaga atcctactaa gtttgagtat
     6361 tatggcattg gaaaagctgt tttcttctat catttgttct gcttgtaatt tactgtgttc
     6421 tttcagtttt tgttttcgga catcaaaatg caaatggatg gataagagtt aataaatgat
     6481 atggtccttt tgttcattct caaattatta ttatctgttg tttttacttt aatgggttga
     6541 atttaagtaa gaaaggaact aacagtgtga tattaaggtg caatgttaga catataaaac
     6601 agtctttcac ctctctttgg ttatgtcttg aattggtttg tttcttcact tatctgtgta
     6661 atcaagttta ctatgagtct atgatcaagt aattatgcaa tcaagttaag tacagtatag
     6721 gcttaagggt gggcgcgccg acccagcttt cttgtacaaa gtggttgata acagcggttg
     6781 actagagtta tcaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa
     6841 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
     6901 acatatccag tcactatggc ggcccctcga ggggccgcat taggcacccc aggctttaca
     6961 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccggc gagattttca
     7021 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
     7081 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
     7141 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag
     7201 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt
     7261 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt
     7321 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg
     7381 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc
     7441 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc
     7501 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc
     7561 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc
     7621 gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag
     7681 tggcagggcg gggcgtaaag atctggatcc ggcttactaa aagccagata acagtatgcg
     7741 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     7801 tcaaaaagag gtgtgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     7861 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     7921 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     7981 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg actggtgaaa
     8041 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     8101 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     8161 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     8221 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     8281 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     8341 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     8401 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     8461 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt tgataacagc
     8521 gcttagaggc ttagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct
     8581 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     8641 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     8701 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     8761 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga atcgggaaat ggttccggaa
     8821 ccaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8881 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8941 aactcacatt aggctgaatt aggcgcgcct atttctgaat tcaacacatt gcggacgttt
     9001 ttaatgtact gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt
     9061 ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa
     9121 gggtttctta tatgctcaac acatgagcga aaccctatag gaaccctaat tcccttatct
     9181 gggaactact cacacattat tatggagaaa ctcgagcttg tcgatcgaca gatcccggtc
     9241 ggcatctact ttagtatata gtccttccat caccctctgt gataatctcc ttaaaacttg
     9301 ctcccccagg aatcataggc agaacgtgct cctggtgggg aactatcaca tccagtaaat
     9361 aaggaccggg agaatctagc atagtttgaa ttgctgctct tagttcatcc ttcttcacca
     9421 cacgcgcatt tggcaccttg aaaccgtctg caactgtaca gaagtcgggg tagatctgct
     9481 cctcgtgatc tggatcacct aagtaagtat gtgctctgtt ggccttgtag aatctatcct
     9541 ccaattgtac gaccattcct agatgttggt tgtttaagat catgatcttc acgggtaact
     9601 tctccacaaa aattgtagcc aactcctgta cgttcatcac aaagctacca tcgccatcga
     9661 tgtcgatcac aggaatatct ggttgagttg cggcagcccc caaagcagaa ggaagtccga
     9721 atcccatggc tccaagacct ccagatgtca accaacgccg cggttcgttg tacttgaacc
     9781 actgggctgc ccacatttga tgttggccaa caccagtaga tatgatggca tttccaccag
     9841 tgagttcgta cagcgtctgt atggcagatt gaggagcaat ggcatcctcg aatacgggaa
     9901 acttcattgg ccatttctgc ttttgttctt ctagttctgc cacccagttg gagaagtcga
     9961 aagtcctctt ttgactcttc aagagggcat tgatttcact tagcgcaggt ttcacatcag
    10021 cacacatgga cacatggggc tgtttgttct ttccaatttc agcagggtca atatcgatat
    10081 gaacgatgct ggacctgcta gcaaaagtct ccagtttacc agtcactcga tcgtcgaaac
    10141 gcactccaaa tgccaacaac agatctgctt ggtccacggc gtagttggcg taaacagttc
    10201 catgcattcc aagcattttc aaacttaatg gatcagactc gggatacgct cccaaaccca
    10261 ttaaagtcga tgtgatagga attccggtaa gctcgacaaa ttccctcaac tcttcgctgg
    10321 cgtgattgca acctcctcca gaatacacta caggcttctt agaactagaa atgagtctta
    10381 taatctgctc gattaacgtt ggttccggtg gccgaggcag tcgttgtaag tagcccttca
    10441 gttgcattgg ctgagaccaa tctggaatag ccatttgctg ttgcacgtcc ttgggaatat
    10501 ctaccaagac tgggcccggc ctacctgaag ctgccaaaaa gaaagcctcc ctgattacgc
    10561 gagggatgtc atcaatgttc ataacaaggt aattgtgctt ggtgatcgat ctcgtaacct
    10621 ccacaattgg agtttcttga aaagcatcgg taccaatcat cctactggat acttgacctg
    10681 taatggccac tagtggcaca ctgtccaaga gggcatcagc gagcccggta acaagattgg
    10741 tcgctccagg accggatgtg gctatacaaa cccctactcg tccagacgat ttagcataac
    10801 cttcagcagc gaaaatctcc ccctgctcgt ggcgacagag aatgttgcgg atgctgctgg
    10861 aacgtgtaag agcctggtga atctccatag acgctccgcc gggataagcg aacaccgttc
    10921 taatcccttc tcgctcaaga gcctccacca aaatgtcggc tcctttgcgg ggctcgtcat
    10981 ccttgtagcg agactgaaag tcggacgggg ttggtgcaac gagtgacttc ccgctgctaa
    11041 gccgtggtcc actcacatcg actgcagact ggatcttctc gctaacagct tgggctttca
    11101 cttccagtga acgctcagtc ttccgagagg tgttgtatgc atgtcccctc agagcggctc
    11161 ccgagaagaa accactcctc acctttagct ctttagctcc cttgcaacaa gaccttgccg
    11221 atggctgcac cttctccagg ctcgctatgc tcaaaggcac agctgcgcat ccactaattg
    11281 acgccatatc tcattgcccc ccggatctgc gaaagctcga gagagataga tttgtagaga
    11341 gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag
    11401 gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca
    11461 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
    11521 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
    11581 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
    11641 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
    11701 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
    11761 gtcgtgctcc accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt
    11821 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
    11881 catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct
    11941 tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc
    12001 ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt
    12061 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc
    12121 aatacgcaaa ccgaattcag aaataaattc agcctaattc ggcgttaatt cagtacatta
    12181 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
    12241 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
    12301 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
    12361 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
    12421 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
    12481 tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc
    12541 aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
    12601 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct
    12661 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
    12721 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg
    12781 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
    12841 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
    12901 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
    12961 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
    13021 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
    13081 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
    13141 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
    13201 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
    13261 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
    13321 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
    13381 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
    13441 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
    13501 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa
    13561 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
    13621 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
    13681 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa
    13741 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
    13801 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
    13861 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
    13921 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
    13981 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
    14041 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
    14101 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
    14161 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
    14221 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
    14281 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
    14341 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
    14401 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
    14461 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    14521 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    14581 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
    14641 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
    14701 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
    14761 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
    14821 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc
    14881 cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg
    14941 ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg
    15001 gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac
    15061 agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg
    15121 gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg
    15181 tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca
    15241 atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt
    15301 tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg
    15361 cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc
    15421 ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg
    15481 gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg
    15541 gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg
    15601 gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca
    15661 gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg
    15721 ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta
    15781 cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc
    15841 tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac
    15901 acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg
    15961 gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg
    16021 gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca
    16081 cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg
    16141 tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc
    16201 gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct
    16261 tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg
    16321 gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat
    16381 ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc
    16441 ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt
    16501 ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa
    16561 ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat
    16621 agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc
    16681 tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac
    16741 gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg
    16801 ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg
    16861 cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg
    16921 cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt
    16981 acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca
    17041 cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca
    17101 ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt
    17161 cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta
    17221 cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga
    17281 caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat
    17341 cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg
    17401 cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt
    17461 ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa
    17521 gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc
    17581 caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag
    17641 ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc
    17701 ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct
    17761 tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc
    17821 tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt
    17881 gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg
    17941 ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg
    18001 gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg
    18061 attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc
    18121 ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg
    18181 atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc
    18241 actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg
    18301 cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg
    18361 gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca
    18421 gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt
    18481 atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc
    18541 gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag
    18601 ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg aggggaaccc
    18661 tgtggttggc atgcacatac aaatggacga acggataaac ct
//
