LOCUS       sequence-140032-        1061 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 1061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3135
FEATURES             Location/Qualifiers
     source          1..1061
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             638..658
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(701..729)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gtaagtttaa acatgatctt actaactaac tattctcatt taaattttca gagcttaaaa
       61 atggctgaaa tcactcacaa cgatggatac gctaacaact tggaaatgaa ataagcttgc
      121 atgcctgcag gccttggtcg actctagagg atccccgggg attggccaaa ggacccaaag
      181 gtatgtttcg aatgatacta acataacata gaacattttc aggaggaccc ttgcttggct
      241 agcaaaaatg cataaggttt tgctggcact gttctttatc tttctggcac cagcatccgc
      301 actggcagtc tccgaaccgg cctgcaggtc gattttttgc aaattacgag cgttgtaggg
      361 ggcggagcga taggtcctat aggttttggt atatcatcat tcattcattc attggtacat
      421 tcatttaccc accttcctct ttctgagctt ctctggagtt ctgtgcttcc tttttccctt
      481 atctttatac tgtaattttt aactttcagg cattgattgg atccccggga ttggccaaag
      541 gacccaaagg tatgtttcga atgatactaa cataacatag aacattttca ggaggaccct
      601 tgcttggagg gtaccgagct cagaaaaaat gactgctcca aagaagaagc gtaaggtacc
      661 ggtagaaaaa atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      721 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      781 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      841 ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc agccaacact
      901 tgtcactact ttctgttatg gtgttcaatg cttctcgaga tacccagatc atatgaaacg
      961 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
     1021 caaagatgac gggaactaca agacacgtaa gtttaaacag t
//
