LOCUS       sequence-140048-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid encoding mCherry, codon optimized for E. coli, 
            transcriptionally driven by orthogonal T7-lac promoter variant 5F5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-5F5-mCherry
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(40..87)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     83..101
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(140..184)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ttcgccaatc cggatatagt cgactcctcc tttcgctagc aaaaaacccc tcaagacccg
       61 tttagaggcc ccaaggggtt atgctagtta ttgctcagcg gtggcagcag tttaggttaa
      121 ttaagctgcg actagtagac gagtccatgt gctggcgttc aaatttcgca gcagcggttt
      181 ctttaccaga ctcgagggta ccttatttgt acagttcatc cataccgccg gtagagtggc
      241 ggccttctgc acgttcgtac tgttcaacga tcgtatagtc ttcgttatgg ctggtgatat
      301 ccagcttgat gttcacgttg tatgcacccg gcagttggac cggcttttta gccttgtacg
      361 tggttttcac ttctgcgtca tagtgaccgc catccttcag tttcaggcgc tgcttgattt
      421 cgcctttcag ggcaccgtct tccgggtaca tacgttcgga actcgcttcc cagcccatcg
      481 tctttttctg cataaccgga ccatcgctcg ggaaattggt gccgcgcagc ttcactttat
      541 aaataaattc accgtcttgc agagagctat cctgcgtgac ggtaaccaca ccgccatctt
      601 cgaagttcat aacacgttcc catttgaagc cttccggaaa ggacagtttc agatagtccg
      661 ggatatcggc cggatgctta acgtacgctt tcgaaccata catgaattgc ggtgacagaa
      721 tatcccaagc aaacggcagc ggaccgcctt tggtgacctt cagtt
//
