LOCUS       sequence-140068-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express N-terminal part (1-I210) of hTRAFD1 (FLN29) in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Witwicka H, Jia H, Kutikov A, Reyes-Gutierrez P, Li X, Odgren PR
  TITLE     TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast 
            Acidification and Resorption.
  JOURNAL   PLoS One. 2015 May 19;10(5):e0127537. doi: 
            10.1371/journal.pone.0127537. eCollection 2015.
  PUBMED    25992615
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-19"; volume: "10(5):e0127537. doi"; pages: " 
            10.1371/journal.pone.0127537. eCollection 2015"
COMMENT     Sequence Label: hFLN29-N/pCMV-14-FLAG (1-I210)
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..82)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(761..860)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(790..810)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccacccggga tcactacttg tcatcgtcat ccttgtagtc gatgtcatga tctttataat
       61 caccgtcatg gtctttgtag tcagcccggg atccaattga aacctgggct gtaatgttcc
      121 tttggttggt agttctattg tggaaaacat ctgattcaaa ggctctcagg ggccttcgcg
      181 gcagcctcat gggtgggtcc agagcctcaa tttgtctgag gagttgggat gcaatccaga
      241 ttccatcctg accccaagat tcatcatatg cattaggagg tatggcaacc tcatttctct
      301 tttcctcccc ctctctccca caaacttcag ggtgagtctt cagatctttc acaaggacat
      361 tgcgaccaca gttgccacat agttccgtcc gggcaccaca ataatcttca tgttccttca
      421 gtttgagaat ggaaagttct aaatcacagt gctggcagac agcaagccgc aaagggcact
      481 cagtctcctc atgcttcttt aacagcctct tctccaactt cttgttacat ttgcaggtca
      541 cctgacagtg ttctgcagcc atgtgagtct ccatgtcaga tttgggaaat ggttccttac
      601 aggtaggaca cataccaatg ttcctttgac agtggatctc atggatggta aagttaaaca
      661 caggaatttc ttttttgcag ttgtcacaca gtcgagtttc ctggtcatct agaaattcag
      721 ccatgcggcc gcaagcttaa ttctgacggt tcactaaacg agctctgctt atatagacct
      781 cccaccgtac acgcctaccg cccatttgcg tcaacggggc ggggttatta cgacattttg
      841 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
      901 ttggaaatcc ccgtgagtca aaccgctatc
//
