LOCUS       sequence-140077-         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express middle part (L175-D390) of human TRAFD1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Witwicka H, Jia H, Kutikov A, Reyes-Gutierrez P, Li X, Odgren PR
  TITLE     TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast 
            Acidification and Resorption.
  JOURNAL   PLoS One. 2015 May 19;10(5):e0127537. doi: 
            10.1371/journal.pone.0127537. eCollection 2015.
  PUBMED    25992615
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-19"; volume: "10(5):e0127537. doi"; pages: " 
            10.1371/journal.pone.0127537. eCollection 2015"
COMMENT     Sequence Label: hFLN29-Mid/pCMV-14-FLAG (L175-D390)
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..81)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(781..880)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(810..830)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 acccgggatc acctacttgt catcgtcatc cttgtagtcg atgtcatgat ctttataatc
       61 accgtcatgg tctttgtagt cagcccggga tccgtcacac tggtcctggt gatggaacag
      121 cacctcctct tccagctgta ccccacagaa ttcacatggg ataatgatgc tctcctccac
      181 agggtgtgaa ttgctcaggc ccatcaaaga gtctaactgg ttacttgcag cctgttgcaa
      241 gaagttctgg aagatgacat caggttcctc cacccctctg ggggaagagc tgccagtatt
      301 gagtgaaggt aaggcacgtg aagggttaca gcttgtctga tggtcaatca gcagttcctc
      361 tgggtagagc tcctcacaaa attcacaagg caacatgatc tcgtcagctg caccctttat
      421 gtcactgaga gacctgggac cgccatgaga ctggtcggcc tcacatacgg ccctccagaa
      481 gtcctgctct gccacactgg aggcttggcc ttcattttgc agacttaggg ccaacatgaa
      541 gtccaagttt gcactctctt caccaccctc tttggggggc tgttggcctc tattcctttc
      601 ctgcctctct tgttcttcaa acagattatt ctgaattgaa acctgggctg taatgttcct
      661 ttggttggta gttctattgt ggaaaacatc tgattcaaag gctctcaggg gccttcgcgg
      721 cagcctcatg ggtgggtcca gcatgcggcc gcaagcttaa ttctgacggt tcactaaacg
      781 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaacggggc
      841 ggggttatta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt
      901 gacgtcaatg g
//
