LOCUS       sequence-140080-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of HA tagged Ubiquitin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  TITLE     Lentiviral expression
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti puro HA-Ubiquitin
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             64..90
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             100..327
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             100..327
                     /codon_start=1
                     /gene="Homo sapiens UBB"
                     /product="human ubiquitin B"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     misc_feature    409..526
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtcgccacc
       61 atgtacccat acgatgttcc ggattacgct agcggatcca tgcagatctt cgtgaaaacc
      121 cttaccggca agaccatcac ccttgaggtg gagcccagtg acaccatcga aaatgtgaag
      181 gccaagatcc aggataagga aggcattccc cccgaccagc agaggctcat ctttgcaggc
      241 aagcagctgg aagatggccg tactctttct gactacaaca tccagaagga gtcgaccctg
      301 cacctggtcc tgcgtctgag aggtggttat tagccggact cagatctcga gctcaagctt
      361 ctctagagaa ttctcgacct cgagacaaat ggcagtattc atccacaatt ttaaaagaaa
      421 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
      481 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
      541 ggacagcaga gatccacttt ggccgcggct cgagggggtt ggggttgcgc cttttccaag
      601 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
      661 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
      721 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
      781 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
      841 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
      901 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
      961 cggggtgtgg ggcggtagtg tgg
//
