LOCUS       sequence-140081-       11866 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11866)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 11866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS262
FEATURES             Location/Qualifiers
     source          1..11866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(214..233)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     333..355
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(393..411)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        479..583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(584..652,653..1444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(802..821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1615..2203
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2104..2123
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3839..3850)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5881..5897
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5881..5897
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5917..5937
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    8380..8404
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8413..8433
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9802..9873
                     /codon_start=1
                     /product="nuclear localization signal from the C. elegans 
                     EGL-13 transcription factor (Lyssenko et al., 2007)"
                     /label=EGL-13 NLS
                     /translation="SRRRKANPTKLSENAKKLAKEVEN"
     protein_bind    complement(9877..9901)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10334..10354)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(10362..10379)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10362..10378)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cacgtgacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
       61 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      121 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
      181 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
      241 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
      301 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
      361 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
      421 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
      481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      601 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      661 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      721 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      781 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      841 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      901 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      961 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     1021 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     1081 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     1141 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     1201 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     1261 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     1321 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     1381 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     1441 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     1501 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     1561 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     1621 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1681 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1741 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1801 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1861 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1921 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1981 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2041 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2101 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2161 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2221 ctttttacgg ttcctggtct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2281 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgatacca gcggataaca
     2341 atttcacagg gccctgtcgg attatgggct cttcttttat cctctacttt ctttctagaa
     2401 aatgcaaatg ttgagtcaga aattgctgaa tggagtacaa ctgcaatggg tttagcttat
     2461 gtttctggaa caattactgg tggaattgtg attgaaaagt aaggttatat ttcatttcaa
     2521 taaatgttac cacagtataa caaaaacaag ttacattgtt cagagtatgt ctgaatgtat
     2581 tattattatt gtatttgtgc atcagtactg tagttgtact gaagaagggt ttaaaataat
     2641 ctcaacgttt ttaagacgag ttagtgagaa tacggtcgca gttaggatgt ttggacatag
     2701 ttttattaga taatcgtgaa ctaactcact ataaaaaccc gctaactcat tgaataagat
     2761 agttttaaac gttcaaaatt tgaagacaaa attgtatgaa taagtttaca gagttggtcg
     2821 acgcaagctt ctccttctct tcacatttct caataattta gctctaattg cattcgtatt
     2881 cttcgcaaaa atacgtattc tgattgaccc gatgaaatac ggatgcctag ttgcattgat
     2941 catctacggt tatacttatg ggtgaggttt ttcaaatata ttttgattta atcgaataat
     3001 gaaatttcag aactggagtt ggtccaattt cgtggtttat atcctctgaa ctggtacctc
     3061 aaaaacatcg tagtattgct caatctgtgg catattcaat gaatactgta atggtggtta
     3121 tcagtacatt tacagtatta cctctatatt ccgttattgg tagttatgct ttccttattc
     3181 tatattcaat tccttcattt ataagtatgc ttatattatt tcgctactta ccggaaacca
     3241 aaggacgaga gattcatgat attgtgaatg aacttaaaaa aaaatgaaaa cctttgaaaa
     3301 cgttcatacg gccgaaattt tacttgatcg agccaattga acttttgtta agacttacag
     3361 aaaagtgaga gattttgtta caaaaaagtg agaaaaaagc gaaaatgaac aggaaaaaga
     3421 agaaaaatac acccgaagta tcatataaaa attaaaaaga ttgtttgacc tggcggaact
     3481 tttttgcaaa aactctgaat atccatggca catgcttcgt gcaaaacagg atccaaatga
     3541 atatcaacaa gagattcttg aagacgacga gccacttgct cagagcattg cttatctcga
     3601 atgagaccct tgttaaaatc atttttcaaa cattatatga cattgtcgaa atgtcctcct
     3661 gattccatga tggtagcaaa ctcacttcgt ctttgagcca atttatccaa gtccttgtaa
     3721 aaagtattcg aaaattgtta acggataaaa tgtttattat aatatcaaaa acaatttgtc
     3781 agttgaccac tttttgatat atttgacaga aaccgggatg aattggctca aagttagggc
     3841 gcccttctat tacagggttt ctgataacaa accggttatt aactcccaac aagggatgat
     3901 ttcaattcat catgctcaaa ttgacccaaa ttaagttaca tgacaaattc atcgcccttt
     3961 tcaactcttc ttggctcatc atctgttatt ctgttcctat tctgtctgca ccctataccc
     4021 ctttgcatac tctctcgtca ttcctttctt ggatagtgtc ttcttctccc cagctctgct
     4081 acttctatga cttgcgcccg ctgcttttcc gctcgttcct ctcctctctg acgtcttctt
     4141 ctgcttcttt tctccttcct ttttcccttc tcaatcttcc tctttttccc atttctgtca
     4201 atcattcgaa gaagaagaag aagaagaccc tcattcattt catttttttt tctgtcggtg
     4261 tgtgctgctc ggttaagagt gagctctcta ttccacgtct tcttcttttt cttcttcgat
     4321 tcgaatcaat cactccacca aaacgcattc gtttttggga ttcaccccgc ggttcgcaac
     4381 aggtttcttt ttcaaattat tagcgttata aatagaaaaa tggtggagtt tcaataaaaa
     4441 tgataattta caaagtgtat tttgattaca tgtactcaaa aaggttgaaa tttttcatac
     4501 cagttttccg gaaatccatc tgatatcatt atcgtatttt tctttttaaa aatgttttca
     4561 aaaaaaaaca aaatatagct ggttatttgg caccctctaa ttaccatttt cctgtcacac
     4621 cacactcttt tccttcttcc acttctttcc gttttcagcc gcttccaacc aaaccgatat
     4681 gaaagccgag caacaacaat cgattccacc cggctcggcg accttcccgt cgcaggtgag
     4741 actcagaaaa ctagagaaac cgctcaacta actcttgata tccgatttca ttcttttctc
     4801 ttttcttttt gttgaacttt tccatatttc cagatgccac ggccaccacc aagcaccgaa
     4861 caaggaatca caacggaatc ggagcttgcg aagaaagctc aaatcactcc gaacgacgtt
     4921 ttagcacttc cgggaatcac tcaaggtatc ttgctccctt tggatttcag tttaaaacat
     4981 aaatttagga ttcttatgct ccccatctgc gaacatctat aacatcgagt tcaccaagtt
     5041 ccaaatccgt gatctggaca ctgagcaagt gcttttcgag atcgccaaac cggagaacga
     5101 tcaggagaat gatgagtcgc cacaggagtc ggcaagatac gtgcgttata gatttgctcc
     5161 aaactttttg aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag gagacatccc
     5221 aatccatcat ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc tgaagtgttt
     5281 tgactttgaa ttcggattct gtattccgaa ttcacgaaac aactgtgaac atatctatga
     5341 gttccctcaa ctctctcaac aactcagtga gtcattattc taaaaagtac aattcaaaag
     5401 actaatctct ttttcagtgg acgacatgat caacaatcca aacgagactc gttctgacag
     5461 cttctatttc gtcgataaca aactcgtcat gcacaacaaa gccgactact catatgatgc
     5521 ataaatattt aatacaaaaa tgttctggat aattattctg tcgaatagaa aaaaaactcc
     5581 aaatgtgatt aaattccaat aattcctgtc tagtttgttc cttccccttc cccttctcat
     5641 gttcaatgca ttcctaagct tttcagttcc ccccttgttt ctatattttt tcgctgtcct
     5701 gtcacactcg ctaaaaacac taatcacacg gaaatctgtt ttcaataaaa actccaactt
     5761 taactcattt tcaatttcaa ctgaaagatt ttttcattag agaatgtcta gaactaggcc
     5821 cgggctacgt aatacgactc acttaaggcc ttgactagag ggtaccagag ctcacctagg
     5881 caggaaacag ctatgaccat gattacgcca agctatcaac tttgtataga aaagttgttc
     5941 cttcagaaga cgtgctttcc tttttcttct cgtatcgatc ccccgtatgg aaggctcctc
     6001 gtttcaacag ccaacccctt cccattcgtt ttcgtcgtcc gtcttcttct tcgtcttctt
     6061 ttttcttttc ttttcctttt tctgctggtt tttgcgagaa agagtgtatg cgagaaaagc
     6121 cagttgggga aaagaagaag aagcggtggg aggctcgacg gcgcggagca cacttcttct
     6181 ctgagtctgt ctcttgctca cgactccagt ccaacatctc cagcttctca ctaaaacgtt
     6241 ttcttttttg ctcgtgtgtc gtgtgctctc aacaaaccgc taaggactat aaaatattga
     6301 ataggtgcca gcactttttt gttctagttc cagaaaaagt atttcttgta ttggattttc
     6361 gattgcttca ccgttacaga taatttttca ttgtgaaaaa tatcaatata tgggggtatt
     6421 ttgaaaaatg atattttcat atcgagtttt ttcaaaatca gtttggtttt gttggatatt
     6481 gttcaaaacg cattttgccc aaaaaataaa tattgaaact ttacaaacat tatttgtagc
     6541 cccgttccat gcctattcct attttccgtt aaaagccaaa aatacaaata aaaatgaaaa
     6601 atctgttttt cgaacaatgg tattttggtc tcatcacact tttgactctt ctcaattatg
     6661 cttcgaaata ggaacgaatt caaatttaac taatggcact tggggtgcat cttcttttga
     6721 aacagaaaac tgacctttca cccaaatgaa aattacatga gcttgtgaag atgacacagg
     6781 tcagtaaaaa gaaaaaagtg cgtgggtatt tctttctcca taattcttga aatttcattg
     6841 gagatatttt gaagaggtgg gctcatctaa acattaagct taagattttt gaaatatttg
     6901 aaagacccaa aataacttac acaatctttt caacaaaaaa aactgtcatg aatttttatt
     6961 tctgaaaagt ctccaacatt gtcgacaaag gaattgattt ttaatgtctt caaataattt
     7021 agaagccgac aaatcaaaac tgttgatgcc cttcgctcat ctctgattta tcatcgtatt
     7081 tctcaatttc ccgcgaagtt cgaatttaga catgttccaa ggtctccgaa acttttcacc
     7141 ccagaacatc atacaaatat tttatacaag cttgaaaaaa tgttactaag aaattttatc
     7201 tccagcaacc taacatatag ttactagaaa cattgttggc tgccaagttc tgagaaacac
     7261 tacaattttt aaagtttttc cgaaaacttt tttaaatctt taagtaactt atccattctc
     7321 tcagttcttg aaatatgtta gtgacaagtt ttaaatattt ttcccaagcc tatcttgcaa
     7381 aactcctgaa ttagaatcat tattcaaagc catgggaaaa tcaagaatgc aaccaccacc
     7441 gcaaatgctc gatgtgagct ttccccaaaa tttgcatcct ctacaacact tctttttcac
     7501 ctgccctccg aaagagcacc tcttctcttc ttttttttgc tctgacgacg acatctaaca
     7561 aaaaatcaat tttttttcgt ttttccaagc ggtcctttcg tgtagtcgac ccattcgtat
     7621 tatcattcga tgctttttca cttcttattt ttcgtcgctt ttgaattgca tatagcgtgt
     7681 ttatcgcaac gaaaaacaga caaaaaagaa agcaaatatg caaaaacaaa aaaatacgaa
     7741 gccaataaaa aatatctatc ttcaattgga attcgaattt tcaattcaaa ttcaaaccta
     7801 ctaaaaccta ctattttcca taattataga ttctcaaagg tctccaaagg aatgagccaa
     7861 gaacaaaaag ttgaggagaa tactgcacat ttccctttct ataacttttt caagatctac
     7921 taagattttt gacccttggg atcctcaaag ttttcaaaaa gtaatcctgc tcttctctaa
     7981 atgacgtttt tttttttgtt ttcatctgtt caaaaaaccc cttaactagt cgtcattctt
     8041 gcccttgaat aataggataa tatgattaat ctaatgtcat aataatctcc tcttcgtctc
     8101 ccatattttt tcccatacaa ggatttcatt tttacatccg tgtttctcgt atcagaatgt
     8161 attcttcttc tggaaaagta aacagactcc tacatctctc gcctctttct gaaaaattct
     8221 aattatttcg cttctgtaaa cacattttca tccggggttt gcctattttc ccattcgacc
     8281 cccgactccg cctcttcatt ctctcacgga acccttttct tcgacgcttt gcatcttgat
     8341 ttacctctga tttcatttat caatcaaact ttcagtcacc caagtttgta caaaaaagca
     8401 ggctaaaaaa tgccaaagaa gaagcgtaag gtcccacaat ttgacatcct ttgtaaaacc
     8461 ccaccaaagg tcctcgtccg tcaattcgtc gagcgtttcg agcgtccatc cggagagaag
     8521 atcgccctct gcgccgccga gctcacctac ctctgctgga tgatcaccca caacggaacc
     8581 gccatcaagc gtgccacctt catgtcctac aacaccatca tctccaactc cctctccttc
     8641 gacatcgtca acaagtccct ccaattcaag tacaagaccc aaaaggccac catcctcgag
     8701 gcctccctca agaagctcat cccagcctgg gagttcacca tcatcccata ctacggacaa
     8761 aagcaccaat ccgacatcac cgacatcgtc tcctccctcc aactccaatt cgagtcctcc
     8821 gaggaggccg acaagggaaa ctcccactcc aagaaggtaa gtttaaacat atatatacta
     8881 actaaccctg attatttaaa ttttcagatg ctcaaggccc tcctctccga gggagagtcc
     8941 atctgggaga tcaccgagaa gatcctcaac tccttcgagt acacctcccg tttcaccaag
     9001 accaagaccc tctaccaatt cctcttcctc gccaccttca tcaactgcgg acgtttctcc
     9061 gacatcaaga acgtcgaccc aaagtccttc aagctcgtcc aaaacaagta cctcggagtc
     9121 atcatccaat gcctcgtcac cgagaccaag acctccgtct cccgtcacat ctacttcttc
     9181 tccgcccgtg gacgtatcga cccactcgtc tacctcgacg agttcctccg taactccgag
     9241 ccagtcctca agcgtgtcaa ccgtaccgga aactcctcct ccaacaagca agagtaccaa
     9301 ctcctcaagg acaacctcgt ccgttcctac aacaaggtaa gtttaaacag ttcggtacta
     9361 actaaccata catatttaaa ttttcaggcc ctcaagaaga acgccccata ctccatcttc
     9421 gccatcaaga acggaccaaa gtcccacatc ggacgtcacc tcatgacctc cttcctctcc
     9481 atgaagggac tcaccgagct caccaacgtc gtcggaaact ggtccgacaa gcgtgcctcc
     9541 gccgtcgccc gtaccaccta cacccaccaa atcaccgcca tcccagacca ctacttcgcc
     9601 ctcgtctccc gttactacgc ctacgaccca atctccaagg agatgatcgc cctcaaggac
     9661 gagaccaacc caatcgagga gtggcaacac atcgagcaac tcaagggttc cgccgaggga
     9721 tccatccgtt acccagcctg gaacggaatc atctcccaag aggtcctcga ctacctctcc
     9781 tcctacatca accgtcgtat ctcccgtcgt cgtaaggcca acccaaccaa gctctccgag
     9841 aacgccaaga agctcgccaa ggaggtcgag aactaaaccc agctttcttg tacaaagtgg
     9901 ggatgatcga cgccaacgtc gttgaatttt caaattttaa atactgaata tttgtttttt
     9961 ttcctattat ttatttattc tctttgtgtt ttttttcttg ctttctaaaa aattaattca
    10021 atccaaatct aaacattttt ttttctcttt ccgtctccca attcgtattc cgctcctctc
    10081 atctgaacac aatgtgcaag tttatttatc ttctcgcttt catttcatta ggacgtgggg
    10141 ggaattggtg gaagggggaa acacacaaaa ggatgatgga aatgaaataa ggacacacaa
    10201 tatgcaacaa cattcaattc agaaatatgg aggaaggttt aaaagaaaac ataaaaatat
    10261 atagaggagg aaggaaaact agtaaaaaat aagcaaagaa attaggcgaa cgatgagaat
    10321 tgtcctcgct tggcaacttt attatacata gttgataatt cactggccgt cgttttacac
    10381 tcgagacgta cggtgcgcgc gatgcattcg aagatctgcc cactagtgag tcgtattata
    10441 agtgcaagta agatcagtgt ttgtttcgat atcagtctgt ttcgtaacgg tcttctgtat
    10501 aactacaaaa aagaataaaa aaccgtatca caactgagaa aaaagacaaa acttattgaa
    10561 cagtgttcct ttcaaaaaat tattttgtaa aaaatcgagt ttttctccaa aataacgcac
    10621 gcaatcaaaa acaagaaaac taacgtgttc tcctgggcgt ggaactcctt atcaagatta
    10681 ccattattgt ctgtcagaat tgatatttca attatttcat atctagacaa acccgggtgt
    10741 cttgcattta ttagtactgt gagtttacat acaaacgagg cttactcaga taacatagag
    10801 tcacctctaa ttagcaaaaa cccatttcta attagctatt tcgaccagtt ctaaagtcac
    10861 cgtaattaga aatggttttt gtagcttttc taattagaaa tgaccccgag ataagtgaaa
    10921 tatggcattt tctgggtttt ctagtaagaa atgatcccgc acgtagtgat tccgcgttac
    10981 aagccaaatt caaattaaaa aagtatatta cacaattcaa gttacattat tttcaggaaa
    11041 caatggcgaa gagcaaagca gagattcaaa aggaggaaag agacaacaga tatgaacgaa
    11101 gacttcttga aaagagaaag agacatgagg tgagtaacca tgataataaa taatcaagta
    11161 agggctatag atttataaat tctggagtta acagtttcga aaaaaagtag ttttcatatg
    11221 tgcgaaggaa tatcaaaaat aggggaatag cctaaacgag tgagaaaatt aaatgtttgt
    11281 tattttttca aaaatgtctg cagggccgtt atcctttcaa atgcactcta tcaaacatag
    11341 atcatatcca tcatatctta tccagaattt atatcatatc caagattgag agcgtttcaa
    11401 ggaaaatctc aaacaaccat acaattttgt agatcgacaa caaggcctcc acttcttcat
    11461 cgatggcctc tgcagcacct gcaccaccaa gtttgtattt ttcatattaa tataaagtga
    11521 gccacgcata gtcgttttta ggatttttgc cagatcgatg tcgcgcaaac agactgtttt
    11581 tttttctttg aaagccgtgc gtgacatggg ccacggattg taacgccgcg ctggatggat
    11641 aaaaatttat tcagtttttt agcctttaca aaatatggtt cgactacttt ctttattttt
    11701 acatttttat aggtgctcat tgagctgaga tattttgggt ataatcggaa aatcgctgat
    11761 tttggtcatt tttctgcact aaagattaca caaaaagttc agtatttaga ttcattgttg
    11821 aaaattgggg atgatctaca gatacatatc gccgtgttca aaaaca
//
