LOCUS       sequence-140084-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express C-terminal part (Q391- end) of human TRAFD1(FLN29) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Witwicka H, Jia H, Kutikov A, Reyes-Gutierrez P, Li X, Odgren PR
  TITLE     TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast 
            Acidification and Resorption.
  JOURNAL   PLoS One. 2015 May 19;10(5):e0127537. doi: 
            10.1371/journal.pone.0127537. eCollection 2015.
  PUBMED    25992615
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-19"; volume: "10(5):e0127537. doi"; pages: " 
            10.1371/journal.pone.0127537. eCollection 2015"
COMMENT     Sequence Label: hFLN29-C/pCMV-14-FLAG (Q391-END)
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..82)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        complement(713..916)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(742..762)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccacccggga tcactacttg tcatcgtcat ccttgtagtc gatgtcatga tctttataat
       61 caccgtcatg gtctttgtag tcagcccggg atccttgctc ctcctcttcc tcttctgcat
      121 ccccagctcc ctgttgcttg gaaggctttg cctttgcagt tctgctgcgg tagttggcag
      181 ctgcaggggt gacccgggaa ttcctgccac cttcactcct accactagct ccgtatctcc
      241 ctgaaggccc aggggagaaa gagggcacaa tgttttctgg gtagagattt tgaggagggc
      301 gaatcactga aacgtgccca ggggctatgg ccccattctg gctgtctcga ttccgcccct
      361 ggatgtcctg gctgtctgag ttgctgagct tcggcacaca aggagagctg ggctggcacc
      421 caggtctggg gcctgatgtt gatctcgata gctggttata ggtagctgtc atattgttaa
      481 tgggtcggct gggaggcaga caggaggtgg gcccattagc tgtttcctgc ttagtatcat
      541 ccaggtaacc agaagacagg tctccctggt gtctgacacg cctcctgggc agctctggtg
      601 aggtctcttg aggctgggaa tccagtctag gaatcccctc tgtcacatgg ttggttgcag
      661 tggctgggcg ttgcatgcgg ccgcaagctt aattctgacg gttcactaaa cgagctctgc
      721 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaacggg gcggggttat
      781 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
      841 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttggtgtact
      901 gccaaaaccg catcaccatg gtaatagcga tgactaatac gta
//
