LOCUS       sequence-140087-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces
            ~10-fold higher titer virus. 3rd generation lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Sanjana NE, Shalem O, Zhang F
  TITLE     Improved vectors and genome-wide libraries for CRISPR screening.
  JOURNAL   Nat Methods. 2014 Aug;11(8):783-4. doi: 10.1038/nmeth.3047.
  PUBMED    25075903
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2014-08"; volume: "11"; issue: 
            "8"; pages: "783-4"
COMMENT     Sequence Label: lentiCRISPR v2
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    132..249
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        300..548
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     300..320
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     471..490
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
       61 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
      121 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
      181 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
      241 caaaattttc gggtttatta cagggacagc agagatccag tttggttaat taaggtaccg
      301 agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga
      361 taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa
      421 agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata
      481 tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
      541 gaaacaccgg agacggttgt aaatgagcac acaaaataca catgctaaaa tattatattc
      601 tatgaccttt ataaaatcaa ccaaaatctt ctttttaata actttagtat caataattag
      661 aatttttatg ttcctttttg caaactttta ataaaaatga gcaaaataaa aaaacgctag
      721 ttttagtaac tcgcgttgtt ttcttcacct ttaataatag ctactccacc acttgttcct
      781 aagcggtcag ctcctgcttc aatcattttt tgagcatctt caaatgttct aactccacca
      841 gctgctttaa ctaaagcatt gtctttaaca actgacttca ttagtttaac atcttcaa
//
