LOCUS       sequence-140097-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  UAS induced expression of Drosophila melanogaster Orco cDNA with 478
            Tyrosine to Alanine amino acid change.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Nakagawa T, Pellegrino M, Sato K, Vosshall LB, Touhara K
  TITLE     Amino acid residues contributing to function of the heteromeric 
            insect olfactory receptor complex.
  JOURNAL   PLoS One. 2012;7(3):e32372. doi: 10.1371/journal.pone.0032372. Epub 
            2012 Mar 5.
  PUBMED    22403649
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3):e32372. doi"; pages: " 
            10.1371/journal.pone.0032372. Epub 2012 Mar 5"
COMMENT     Sequence Label: pUAST attB Orco (Y478A)
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    158..191
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    224..318
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        337..575
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     522..543
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 actgaaagtg acatccagtg tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc
       61 ataatagtgt tgtttatata tatcaaaaat aacaactata ataataagaa tacatttaat
      121 ttagaaaatg cttggatttc actggaacta ggctagcata acttcgtata atgtatgcta
      181 tacgaagtta tgctagcgga tccaagcttg catgcctgca ggtcggagta ctgtcctccg
      241 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
      301 ggagtactgt cctccgagcg gagactctag cgagcgccgg agtataaata gaggcgcttc
      361 gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta agcgaaagct
      421 aagcaaataa acaagcgcag ctgaacaagc taaacaatct gcagtaaagt gcaagttaaa
      481 gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac tgcaactact gaaatctgcc
      541 aagaagtaat tattgaatac aagaagagaa ctctgaatag ggaattggga attcgccacc
      601 accatgacaa cctcgatgca gccgagcaag tacacgggcc tggtcgccga cctgatgccc
      661 aacatccggg cgatgaagta ctccggcctg ttcatgcaca acttcacggg cggcagtgcc
      721 ttcatgaaga aggtgtactc ctccgtgcac ctggtgttcc tcctcatgca gttcaccttc
      781 atcctggtca acatggccct gaacgccgag gaggtcaacg agctgtcggg caacacgatc
      841 acgaccctct tcttcaccca ctgcatcacg aagtttatct acctggctgt taaccagaag
      901 aatttctaca gaacattgaa tatatggaac caggtgaaca cgcatccctt gttcgccgag
      961 tcggatgctc gttaccattc gatcgccctg gcgaagatga ggaagctgtt ctttctggtg
     1021 atgctgacca cagtcgcctc ggccaccgcc tggaccacga tcaccttctt tggcgacagc
     1081 gtaaaa
//
