LOCUS       sequence-140099-        1128 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 based UAS modified with a IL2RGTC binding site upstream of it, 
            driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and 
            KRAB(A) repressor domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1128)
  TITLE     Bonkowsky lab FingR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-K...
FEATURES             Location/Qualifiers
     source          1..1128
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(86..97,98..260)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     633..649
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     633..649
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    669..689
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     691..710
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        691..709
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(762..866)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatctgcaaa agacgtgaat atctgttcag acacccatat ccactctgtt ccacacaggt
       61 cagaggtttg tccaggagtt cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa
      121 gtgaaagtac aagtacttag ggaaaatttt actcaattaa aagtaaaagt atctggctag
      181 aatcttactt gagtaaaagt aaaaaagtac tccattaaaa ttgtacttga gtattaagga
      241 agtaaaagta aaagcaagaa agaaaactag agattcttgt ttaagctttt aatctcaaaa
      301 aacattaaat gaaatgcata caaggtttta tcctgcttta gaactgtttg tatttaatta
      361 tcaaactata agacagacaa tctaatgcca gtacacgcta ctcaaagttg taaaacctca
      421 gatttaactt cagtagaagc tgattctcaa aattgttagt gtcaagccta gctcttttgg
      481 ggctgaaaag caatcctgca gtgctgaaaa gcctctcaca ggcagccgat gcgggaagag
      541 gtgtattagt cttgatagag aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg
      601 tgtctgaaac acaggccaga tgggccctcg agcaggaaac agctatgacc atgattacgc
      661 caagctatca actttgtata gaaaagttgg taatacgact cactataggg cgaattgggt
      721 accaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
      781 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
      841 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaca
      901 ttgattattg actagtcttc cacagagtac tagttattaa tagtaatcaa ttacggggtc
      961 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1021 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1081 aacgccaata gggactttcc attgacgtca atgggtggac tatttacg
//
