LOCUS       sequence-140100-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 based UAS modified with a IL2RGTC binding site upstream of it, 
            driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and 
            KRAB(A) repressor domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  TITLE     Bonkowsky lab FingR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-K...
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             493..618
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     intron          661..793
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     776..795
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 cgagcgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtggccaga
       61 tactgcgacc tccctagcaa actggggcac agatctagat ttcagtgcca gatttgcatg
      121 cgcaacttta gccgcaaaag caccctgacc gatcatattc gcacccatac cggcgaaaaa
      181 ccgtttgcgt gcgatatttg cggccgcaaa tttgcggcgc gcagcacccg caccacccat
      241 accaaaattc ataccggcag ccagaaaccg tttcagtgcc gcatttgcat gcgcaacttt
      301 agccgcagcg atagcctgag caaacatatt cgcacccata ccggcgaaaa accgtttgcg
      361 tgcgatattt gcggccgcaa atttgcgcag cgcagcaacc tgaaagtgca taccaaaatt
      421 catctgcgcg gcagccagct gatcgatggt gtcgacgctc ctgaacaacg tgaaggtgct
      481 tctcaagttt ctgtttctgt tacttttgaa gatgttgctg ttctttttac tcgtgatgaa
      541 tggaaaaaac ttgatctttc tcaacgttct ctttatcgtg aagttatgct tgaaaattat
      601 tctaatcttg cttctatggc ttagcgtaca agtaaagcgg ccccggactc gaggccgcag
      661 gtaagtatca aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga
      721 cagagaagac tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc
      781 tttctctcca caggtgtc
//
