LOCUS       sequence-140102-        8620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSTL1-dPSTRy(4,3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Aymoz D, Wosika V, Durandau E, Pelet S
  TITLE     Real-time quantification of protein expression at the single-cell 
            level via dynamic protein synthesis translocation reporters.
  JOURNAL   Nat Commun. 2016 Apr 21;7:11304. doi: 10.1038/ncomms11304.
  PUBMED    27098003
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-21"; volume: "7:11304. doi"; pages: " 
            10.1038/ncomms11304"
COMMENT     Sequence Label: pDA199
FEATURES             Location/Qualifiers
     source          1..8620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(108..127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(787..1578,1579..1647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1410..1429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1648..1711)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1989..2006
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1990..2006
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2053..2300)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     2266..2284
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(2541..2561)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2577..2597)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2733..2960)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(2986..3002)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3817..3835)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3852..3868)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3852..3868)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    3869..3902
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5748..5781
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5782..5800
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     6815..6831
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6875..7585
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLKEF
                     VTAAGITHGMDELYK"
     protein_bind    7604..7628
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(8341..8359)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 taattcgaag gccgcgttgc tggcgttttt ccataggctc ggcccccctg acgagcatca
       61 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
      781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
      841 atccatagtt gcctgactgc ccgtcgtgta gataactacg atacgggagg gcttaccatc
      901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
      961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1081 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgaaa
     1201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1381 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     1681 tcagggttat tgtctcatga gcggatacat atttgaagta tcgttcgaat taattaattt
     1741 cctcaacata acgagaacac acaggggcgc tatcgcacag aatcaaattc gatgactgga
     1801 aattttttgt taatttcaga ggtcgcctga cgcatatacc tttttcaact gaaaaattgg
     1861 gagaaaaagg aaaggtgaga gcgccggaac cggcttttca tatagaatag agaagcgttc
     1921 atgactaaat gcttgcatca caatacttga acttgacaat attatttaag gacctattgt
     1981 tttttccatg taaaacgacg gccagtgcat gcattaaccc tcactaaagg gaacaaaagc
     2041 tgggtaccgg ccgcaaatta aagccttcga gcgtcccaaa accttctcaa gcaaggtttt
     2101 cagtataatg ttacatgcgt acacgcgtct gtacagaaaa aaaagaaaaa tttgaaatat
     2161 aaataacgtt cttaatacta acataactat aaaaaaataa atagggacct agacttcagg
     2221 ttgtctaact ccttcctttt cggttagagc ggatgtgggg ggagggcgtg aatgtaagcg
     2281 tgacataact aattacatga ctcgaggcta gcttatcatt ttttgtgcgc cagacggttg
     2341 cgcagcgccg ctttttcttt acgcagacgc gcgatttctt tttccaggta agcgttttca
     2401 ttttcgatga acgcagcgtc attctccaga gtggtaactt cgttagatct tttaaattcc
     2461 attgtaaaag gtgctgcggc acttccagct tttttatata aggaagtttg attgctgcca
     2521 ccgtcgacac tagatccccc caccttccgc ttcttcttgg gttcagcacc agcccccacc
     2581 ttccgcttct tcttgggttc ggatctcata agctttccct gcattaaaga atcggccaaa
     2641 gccctggggg aagctgtttg agtattaggt gctagggtcg tttttctctt taccaaacta
     2701 actggaagca gccacgcacc tgatttatgg taaccacctc ttagccttag cacaagatgt
     2761 aaggtggact ccttctgaat gttgtaatca gacagcgttc taccgtcttc tagctgctta
     2821 ccggcaaaga tcaatctttg ttgatctgga gggatacctt ccttgtcttg aattttcgac
     2881 ttaacgttgt cgatggtatc ggaagattca acttccaatg ttatggtttt accggtcaaa
     2941 gtcttgacga aaatctgcat gatatcgaat tcctgcagcc cgggggatcc actagttcta
     3001 gaggtctaaa actttctatg ttctattttt ctatttttta tttattctaa cttgtatgct
     3061 agaacacgta tacaaaataa ttattcaaaa tcaataaagg gcttgtttat atactatgag
     3121 tgtgactact cctgaactgc aattctgacc ctctaaggcc aagcagcgtt gaaggggaca
     3181 aaattgaagc taaaacatct cacttaagct ttctcaattt ctctatctac ttcctttttg
     3241 ttacgaatgc cccaatgaaa gccctatcaa aggtgaacca aaagtgtttc ccttaccggg
     3301 atctgcactt tctcagatta ctactatcgc gaagcagcca agcgcaatag ctgcacctgg
     3361 cgaggattaa ccaaccaaaa acggtatata gccgagaaac agtgtacaac cattatttct
     3421 ttctgacgta gtggatggct ggacactttc cgcttatttt agtttcatgc aacgaacaga
     3481 gccgctcgaa caggcgacgt agcgccgaat accccgcgaa acggagatgc tactcaaatg
     3541 caggtagcgg aggcatcgcc gtggggtatg cggtggccga aaaaggacaa agtcggaccc
     3601 ttcatatcag ttaaaatgac gcaagttgag tcaaagtgag ccaaatgggc caaatgcggt
     3661 ggaatagtgg gacaacggca cttatttcgt ccgtacgtcc gttatggatg tgtatactat
     3721 ttcatgattg gcttacctca aagaaaatga aacggatggt gcctcctagc cttggcaatg
     3781 agtatcagta gtggtgttct gcgagctcca attcgcccta tagtgagtcg tattagacgt
     3841 ctcccttcat ggtcatagct gtttcctgat aacttcgtat aatgtatgct atacgaagtt
     3901 attctgtagg cgaacctaac cggccaattt tccaagaccg ctctgccgct gagcttgcac
     3961 ccgtgccgct acacaacacc cacacatccg taaacccccc gccatcggta tcaccaaggt
     4021 tgtctatgac gaggcgtaca agaggacgtg ggggccgcct ctccaatgtc accaatagga
     4081 aaaaagaaag accgagtgcg agtttctgac agaagcctca agaatttttt ttctttcttc
     4141 gactatgctg gaggtagaga tgatcgaggc agtagtcaag tatatataaa ttaattagtt
     4201 ccaccacctt cttttcttgt gttgctcctt tcgatgctat ttctggcttt ttctaatttt
     4261 tttcatcgtt tcttactttc ttcctaatat ataaattctt ttgcattttc attcttcctc
     4321 tccatttgtt cattctcctt aaattttttt taggtctcct ttctttcaag aaacacagtc
     4381 atcgaactat aattaactaa acatgtcagc acctaagaag atagtagtat tacctggaga
     4441 ccacgtagga caggagataa cagcagaggc aataaaggta ttaaaggcaa tatcagacgt
     4501 aaggtcaaac gtaaagttcg acttcgagaa ccacttaata ggaggagcag caatagacgc
     4561 aacaggagta cctttacctg acgaggcatt agaggcatca aagaaggcag acgcagtatt
     4621 attaggagca gtaggaggac ctaagtgggg aacaggatca gtaaggcctg agcagggatt
     4681 attaaagata aggaaggagt tacagttata cgcaaactta aggccttgca acttcgcatc
     4741 agactcatta ttagacttat cacctataaa gcctcagttc gcaaagggaa cagacttcgt
     4801 agtagtaagg gagttagtag gaggaatata cttcggaaag aggaaggagg acgacggaga
     4861 cggagtagca tgggactcag agcagtacac agtacctgag gtacagagga taacaaggat
     4921 ggcagcattc atggcattac agcacgagcc tcctttacct atatggtcat tagacaaggc
     4981 aaacgtatta gcatcatcaa ggttatggag gaagacagta gaggagacaa taaagaacga
     5041 gttccctaca ttaaaggtac agcaccagtt aatagactca gcagcaatga tattagtaaa
     5101 gaaccctaca cacttaaacg gaataataat aacatcaaac atgttcggag acataatatc
     5161 agacgaggca tcagtaatac ctggatcatt aggattatta ccttcagcat cattagcatc
     5221 attacctgac aagaacacag cattcggatt atacgagcct tgccacggat cagcacctga
     5281 cttacctaag aacaaggtaa accctatagc aacaatatta tcagcagcaa tgatgttaaa
     5341 gttatcatta aacttacctg aggagggaaa ggcaatagag gacgcagtaa agaaggtatt
     5401 agacgcagga ataaggacag gagacttagg aggatcaaac tcaacaacag aggtaggaga
     5461 cgcagtagca gaggaggtaa agaagatatt agcatgagaa tattgataaa tttaaatttt
     5521 ggaatgaggg ttgtcctttc ctttacaata gaggcatttg agtgagtagg ttttatcacc
     5581 ttcttgctct tattaaatcg cagttgtctt tccggtatgt gcttgactga ggaacaatat
     5641 tttattcatg atacatttcc atgctacgca tatcgtcatc cacatggaag gtaattttct
     5701 tctgtggcct ttcatatagt tgtgaatggt agaacaatct tcatataata acttcgtata
     5761 atgtatgcta tacgaagtta ttaatacgac tcactatagg gcgaattgga gctcaaaccg
     5821 aaatacagcc atacgtaatt tggtggccta atatgcctgg tgtgctggat aagatattat
     5881 gttaatgaaa tatttgtacg tacatccgtt tgtgcgtatg tatatgtgtg tgtaaaatgt
     5941 acattgtcca agctttccac aactcctatt atcgctagtg ttggcaggat tatttgtttg
     6001 aaaatagcat atatctcgtt atgtgaaact ttgccaaaca atattcctta caacatccgt
     6061 gcatactcta aacccaatca ctaattttca atttttaaaa actttatact ctgtactgcg
     6121 gcaattaagg ctttcgcgtc gttgagcgcg caacactacc gggggttcct taccgtgagc
     6181 ctgcgcctct gtaggagaat gtagaggttc ccgttgaggg aactccagcc atgggttgac
     6241 gggagttccc gtttggcctg attgctcttg cgcagttgac atctagccag aaacagtagt
     6301 agtgggagtt gatattacca atatttacag gtaacctata ttgattaact aaattatagg
     6361 cgtttaaatt tttgggtggt caacaaaaga ggcgagcaac aatttgaatt gaaacgtatg
     6421 tcgcgtgccc attaggaaat gagagaatat tgaggacaac gaggagcttc gcagtgtagg
     6481 aattgagaaa gaagcgacca tttgaacaat gctgaggtta ataagaggcc cacgataaag
     6541 gcgttatatt aatcaacgtt gaacatcttc ccagaaaagt acatgcgaat aaatcaggac
     6601 cgtcgaaaga cgtacggaac atcattggca aatatacatc gggatctgtt ggatatgctt
     6661 gtcgtgatga aatactgttc tcgttgtcgg cttgataaat tttctcccat ttaaaactct
     6721 ttgaaccaaa attgtgtact aacatcaatt tgtatataaa ccatgagtgt atttcacctt
     6781 gtggtttttc acagcttttt aatttagaag aacatctaga actagtggat cccccgggct
     6841 gcaggaattc gatatcaagc ttatgagatc tgcttctaaa ggtgaagaat tattcactgg
     6901 tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat tttctgtctc
     6961 cggtgaaggt gaaggtgatg ctacttacgg taaattgacc ttaaaattta tttgtactac
     7021 tggtaaattg ccagttccat ggccaacctt agtcactact ttaggttatg gtttgatgtg
     7081 ttttgctaga tacccagatc atatgaaaca acatgacttt ttcaagtctg ccatgccaga
     7141 aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca agaccagagc
     7201 tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag gtattgattt
     7261 taaagaagat ggtaacattt taggtcacaa attggaatac aactataact ctcacaatgt
     7321 ttacatcatg gctgacaaac aaaagaatgg tatcaaagtt aacttcaaaa ttagacacaa
     7381 cattgaagat ggttctgttc aattagctga ccattatcaa caaaatactc caattggtga
     7441 tggtccagtc ttgttaccag acaaccatta cttatcctat caatctaaat tatccaaaga
     7501 tccaaacgaa aagagagacc acatggtctt gaaagaattt gttactgctg ctggtattac
     7561 ccatggtatg gatgaattgt acaaagtcga caggtcgaat caaacaagtt tgtacaaaaa
     7621 agcaggctcc gcggccgccc ccttcaccat ggggtcccag aaagttgcgg aactcaaaaa
     7681 ccgtgttgcg gttaaactga atcgtaacga acagctgaaa aacaaagttg aagagctgaa
     7741 gaaccgtaac gcttacctca agaacgaact ggcgaccctg gagaacgagg ttgcgcgtct
     7801 ggaaaacgac gttgcagaat gataatgagc tagcctcgag tgaaaaacct tacccccaaa
     7861 tgtagtgctt tggtgcgatt ccagcatcct gtgatgttca cctacttcaa ggttatataa
     7921 ttttgtgtgt agaattgtaa aaaggatgga tagacagttc ttgagataaa gcacagttat
     7981 tttggtatca catcatcgga gtatttttca attctgattt tttgactgga cccaacaaac
     8041 aactccaatt catccaattg tccttccgta ggtaaacaac tggaaatagg tacacttctt
     8101 tctatcatca atacaacaat gttagtacga gagacttcca gatcaggtat aacgatgccc
     8161 ccagatggac atgtcctata ctcttgacat tcataaacac agagagctga ttcaggctcg
     8221 aaaatattac atcgcatcct taatcgtgct gatgattcca cgggtactac acgtataaaa
     8281 agtttgttcg tagaatactt tttggttggc aaagtcatct gaggtaccca gcttttgttc
     8341 cctttagtga gggttaattt cctgtaaact ttataaatga aattcataat agaaacgaca
     8401 cgaaattaca aaatggaata tgttcatagg gtagacgaaa ctatatacgc aatctacata
     8461 catttatcaa gaaggagaaa aaggaggatg taaaggaata caggtaagca aattgatact
     8521 aatggctcaa cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga
     8581 gggcaccaca caaaaagtta ggtgtaacag aaaatttaat
//
