LOCUS       sequence-140103-        8122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pSTL1-dPSTRr(2,1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Aymoz D, Wosika V, Durandau E, Pelet S
  TITLE     Real-time quantification of protein expression at the single-cell 
            level via dynamic protein synthesis translocation reporters.
  JOURNAL   Nat Commun. 2016 Apr 21;7:11304. doi: 10.1038/ncomms11304.
  PUBMED    27098003
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-21"; volume: "7:11304. doi"; pages: " 
            10.1038/ncomms11304"
COMMENT     Sequence Label: pDA183
FEATURES             Location/Qualifiers
     source          1..8122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     254..271
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     255..271
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(318..565)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     531..549
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(818..838)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(854..874)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(1010..1237)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(1263..1279)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2094..2112)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2129..2145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2129..2145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    2146..2179
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2216..2559
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     terminator      3378..3575
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    3599..3632
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        3633..3651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4666..4682
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4726..5427
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(4865..4883)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5133..5152
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    5446..5470
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6110..6128)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(6416..7004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6496..6515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7175..7966,7967..8035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7798..7817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8036..8099)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taatgatgat atttcataaa taatgtaatt ctatatatgt taattacctt ttttgcgagg
       61 catatttatg gtgaaggata agttttgacc atcaaagaag gttaatgtgg ctgtggtttc
      121 agggtccata atgcttttca attcatcttt tttttttttg ttcttttttt tgattccggt
      181 ttctttgaaa tttttttgat tcggtaatct ccgagcagaa ggaagaacga aggaaggagc
      241 acagacttag atttgtaaaa cgacggccag tgcatgcatt aaccctcact aaagggaaca
      301 aaagctgggt accggccgca aattaaagcc ttcgagcgtc ccaaaacctt ctcaagcaag
      361 gttttcagta taatgttaca tgcgtacacg cgtctgtaca gaaaaaaaag aaaaatttga
      421 aatataaata acgttcttaa tactaacata actataaaaa aataaatagg gacctagact
      481 tcaggttgtc taactccttc cttttcggtt agagcggatg tggggggagg gcgtgaatgt
      541 aagcgtgaca taactaatta catgactcga ggctagctta ttcttcgatt ttcttacgca
      601 gattcgcgat ttctttctcc aggtacgcga tcaggtcttt tttgtgcagg ttctttttct
      661 tcagggtttc gttctcattt tccagagacg caacttcgtt ttcgagctgc gcaaccaggt
      721 tagatctttt aaattccatt gtaaaaggtg ctgcggcact tccagctttt ttatataagg
      781 aagtttgatt gctgccaccg tcgacactag atccccccac cttccgcttc ttcttgggtt
      841 cagcaccagc ccccaccttc cgcttcttct tgggttcgga tctcataagc tttccctgca
      901 ttaaagaatc ggccaaagcc ctgggggaag ctgtttgagt attaggtgct agggtcgttt
      961 ttctctttac caaactaact ggaagcagcc acgcacctga tttatggtaa ccacctctta
     1021 gccttagcac aagatgtaag gtggactcct tctgaatgtt gtaatcagac agcgttctac
     1081 cgtcttctag ctgcttaccg gcaaagatca atctttgttg atctggaggg ataccttcct
     1141 tgtcttgaat tttcgactta acgttgtcga tggtatcgga agattcaact tccaatgtta
     1201 tggttttacc ggtcaaagtc ttgacgaaaa tctgcatgat atcgaattcc tgcagcccgg
     1261 gggatccact agttctagag gtctaaaact ttctatgttc tatttttcta ttttttattt
     1321 attctaactt gtatgctaga acacgtatac aaaataatta ttcaaaatca ataaagggct
     1381 tgtttatata ctatgagtgt gactactcct gaactgcaat tctgaccctc taaggccaag
     1441 cagcgttgaa ggggacaaaa ttgaagctaa aacatctcac ttaagctttc tcaatttctc
     1501 tatctacttc ctttttgtta cgaatgcccc aatgaaagcc ctatcaaagg tgaaccaaaa
     1561 gtgtttccct taccgggatc tgcactttct cagattacta ctatcgcgaa gcagccaagc
     1621 gcaatagctg cacctggcga ggattaacca accaaaaacg gtatatagcc gagaaacagt
     1681 gtacaaccat tatttctttc tgacgtagtg gatggctgga cactttccgc ttattttagt
     1741 ttcatgcaac gaacagagcc gctcgaacag gcgacgtagc gccgaatacc ccgcgaaacg
     1801 gagatgctac tcaaatgcag gtagcggagg catcgccgtg gggtatgcgg tggccgaaaa
     1861 aggacaaagt cggacccttc atatcagtta aaatgacgca agttgagtca aagtgagcca
     1921 aatgggccaa atgcggtgga atagtgggac aacggcactt atttcgtccg tacgtccgtt
     1981 atggatgtgt atactatttc atgattggct tacctcaaag aaaatgaaac ggatggtgcc
     2041 tcctagcctt ggcaatgagt atcagtagtg gtgttctgcg agctccaatt cgccctatag
     2101 tgagtcgtat tagacgtctc ccttcatggt catagctgtt tcctgataac ttcgtataat
     2161 gtatgctata cgaagttatt agcttgcctc gtccccgccg ggtcacccgg cgagcgacat
     2221 ggaggcccag aataccctcc ttgacagtct tgacgtgcgc agctcagggg catgatgtga
     2281 ctgtcgcccg tacatttagc ccatacatcc ccatgtataa tcatttgcat ccatacattt
     2341 tgatggccgc acggcgcgaa gcaaaaatta cggctcctcg ctccagacct gcgagcaggg
     2401 aaacgctccc ctcacagacg cgttgaattg tccccacgcc gcgcccctgt agagaaatat
     2461 aaaaggttag gatttgccac tgaggttctt ctttcatata cttcctttta aaatcttgct
     2521 aggatacagt tctcacatca catccgaaca taaacaacca tgacagtcaa cactaatacc
     2581 tatagtgaga gagcagaaac tcatgcctca ccagtagcac agcgattatt tcgattaatg
     2641 gaactgaaga aaaccaattt atgtgcatca attgacgttg ataccactaa ggagttcctt
     2701 gaattaattg ataaattagg tccttatgta tgcttaatca agactcatat tgatataatc
     2761 aatgattttt cctatgaatc cactattgaa ccattattag aactttcacg taaacatcaa
     2821 tttatgattt ttgaagatag aaaatttgct gatattggta ataccgtaaa gaaacaatat
     2881 attggtggag tttataaaat tagtagttgg gcagatatta ccaatgctca tggtgtcact
     2941 gggaatggag tggttgaagg attaaaacag ggagctaaag aaaccaccac caaccaagag
     3001 ccaagagggt tattgatgtt agctgaatta tcatcagtgg gatcattagc atacggagaa
     3061 tattctcaaa aaactgttga aattgctaaa tccgataagg aatttgttat tggatttatt
     3121 gcccaacgtg atatgggtgg ccaagaagaa ggatttgatt ggcttattat gacacctgga
     3181 gttggattag atgataaagg tgatggatta ggacaacaat atagaactgt tgatgaagtt
     3241 gttagcactg gaactgatat tatcattgtt ggtagaggat tgtttggtaa aggaagagat
     3301 ccagatattg aaggtaaaag gtatagaaat gctggttgga atgcttattt gaaaaagact
     3361 ggccaattat aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg
     3421 tatagttttt ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt
     3481 tttttcgcct cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat
     3541 gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgat
     3601 aacttcgtat aatgtatgct atacgaagtt attaatacga ctcactatag ggcgaattgg
     3661 agctcatgaa agagaatgat taggatgaaa atacaccagc gtggttacgt gctatcgccc
     3721 agcgtgagct ttgccattcc gtctccaata ggaaaaaaaa ttgaaaaaaa aaaaaattta
     3781 aatgcggccc tagctgaaaa aaagatacgt ttcttggaaa aagaccttac catccataca
     3841 ttttggaacg ttacatctgt gcaccacata tttttgatct cagattttag tgtttttttt
     3901 tttggtcctt gttgaacctc ttatttcccg cctacaaagt aatgatcctt actgcggtgc
     3961 tagatggggg ttcagtctct ccaggcagga cgacacgaga atagacgggc tgatcccgtc
     4021 tggcacgcag agacgggagg aaataccaca cagactggcg gttggcgtcc tatccagaag
     4081 tgatatactg tcaatgtact gcatgatata ttcaacggat ggtgtgtatt ttagtacgtt
     4141 tacgcagttt gcgctgttgc tattgccaag ttaagcaaga caccaagaca ataacttgag
     4201 aggtatgtcc ctatatgatg atgattcata attaatggcg accgagggca gcgttgtgct
     4261 agaaagcaat gtattaatat atgagagagc ttcagatagc caatacaccg gaatttagac
     4321 tttgacagga ttgtggcttg ttccagtata acttataata tatgtcaacc tttgagcagg
     4381 aaacccaatg aaaatgcttc attacatata tacgatatgg agaagcatga aaaataacag
     4441 cgtgagatgt tatccatgtt gcaagaacct agtaaaatga attctgaatt tgcaaaaagt
     4501 ccatatttcc acgttctcct cttcctacaa ttttgcgaac gcctgaacaa ccatgcggat
     4561 taccattatt tatattgaca agatggctac ctatgaaaag catagactta ctaacatttt
     4621 ttttttcaaa tatgtttgaa aaacgtggat taatatagtg ataaatctag aactagtgga
     4681 tcccccgggc tgcaggaatt cgatatcaag cttatgagat ctgctagcaa gggcgaggag
     4741 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
     4801 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     4861 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
     4921 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
     4981 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     5041 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
     5101 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
     5161 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
     5221 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
     5281 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
     5341 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
     5401 accggcggca tggacgagct gtacaaggtc gacaggtcga atcaaacaag tttgtacaaa
     5461 aaagcaggct ccgcggccgc ccccttcacc atgggatccg cgcgtaacgc gtatctgcgt
     5521 aagaaaatcg cacgtctgaa aaaagacaac ctgcagctgg aacgtgatga acagaacctg
     5581 gaaaaaatca tcgcgaacct gcgtgacgaa atcgcgcgtc tcgaaaacga agttgcgtct
     5641 cacgaacagt gagctagcct cgagggtaca gtacataaac agatgaattt tatcattctt
     5701 attgttattt ctttgtttac aaaaggtgaa aagtgaaaaa tttcgcaatg gaaaacgtcg
     5761 cgggccgcct ttgagtgacg ttgtgcagga atctcgaaga gtcatcactt tgaagataac
     5821 ggccgctcgc gatgagagaa gacaatgagc attgcttttt tataattttt tataccttgt
     5881 gttaaaataa gtttctttaa ttagttttca cctcttggat gatacaactg taattgataa
     5941 ataggggtag ccataaagaa cagaaaaaag cggcgcaata atttcgtgta ttacaattac
     6001 agttagtcat gagtgaagaa tttcctacgc cgcagctttt ggatgaattg gaagatcaac
     6061 agaaagttac cacgccgaat gagaagagag aggtacccag cttttgttcc ctttagtgag
     6121 ggttaatttc ctgaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     6181 agcttcaatt taattatatc agttattacg cgggaatctc ggtcgtaatg atttctataa
     6241 tgacgaaaaa aaaaaaattg gaaagaaaat gcttcatggc ctttataaaa aggaactatc
     6301 caatacctcg ccagaaccaa gtaacagtat tttacggggc acaaatcaag aacaataaga
     6361 caggactgta aagatggacg catttaatta attcgaaggc cgcgttgctg gcgtttttcc
     6421 ataggctcgg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6481 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6541 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6601 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6661 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6721 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6781 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6841 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6901 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6961 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     7021 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     7081 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     7141 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     7201 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactgccc gtcgtgtaga
     7261 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     7321 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     7381 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7441 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7501 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7561 gagttacatg atcccccatg ttgtgaaaaa aagcggttag ctccttcggt cctccgatcg
     7621 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7681 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7741 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7801 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7861 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7921 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7981 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     8041 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     8101 ttgaagtatc gttcgaatta at
//
