LOCUS       sequence-140104-        8820 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pGPD1-dPSTRy(4,3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8820)
  AUTHORS   Aymoz D, Wosika V, Durandau E, Pelet S
  TITLE     Real-time quantification of protein expression at the single-cell 
            level via dynamic protein synthesis translocation reporters.
  JOURNAL   Nat Commun. 2016 Apr 21;7:11304. doi: 10.1038/ncomms11304.
  PUBMED    27098003
REFERENCE   2  (bases 1 to 8820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-21"; volume: "7:11304. doi"; pages: " 
            10.1038/ncomms11304"
COMMENT     Sequence Label: pDA200
FEATURES             Location/Qualifiers
     source          1..8820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(108..127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(787..1578,1579..1647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1410..1429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1648..1711)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1989..2006
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1990..2006
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2053..2300)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     2266..2284
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(2541..2561)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2577..2597)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(2733..2960)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(2986..3002)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4017..4035)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4052..4068)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4052..4068)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4069..4102
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    5948..5981
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        5982..6000
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7015..7031
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             7075..7785
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLKEF
                     VTAAGITHGMDELYK"
     protein_bind    7804..7828
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(8541..8559)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 taattcgaag gccgcgttgc tggcgttttt ccataggctc ggcccccctg acgagcatca
       61 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
      721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
      781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
      841 atccatagtt gcctgactgc ccgtcgtgta gataactacg atacgggagg gcttaccatc
      901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
      961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1081 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgaaa
     1201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1381 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     1681 tcagggttat tgtctcatga gcggatacat atttgaagta tcgttcgaat taattaattt
     1741 cctcaacata acgagaacac acaggggcgc tatcgcacag aatcaaattc gatgactgga
     1801 aattttttgt taatttcaga ggtcgcctga cgcatatacc tttttcaact gaaaaattgg
     1861 gagaaaaagg aaaggtgaga gcgccggaac cggcttttca tatagaatag agaagcgttc
     1921 atgactaaat gcttgcatca caatacttga acttgacaat attatttaag gacctattgt
     1981 tttttccatg taaaacgacg gccagtgcat gcattaaccc tcactaaagg gaacaaaagc
     2041 tgggtaccgg ccgcaaatta aagccttcga gcgtcccaaa accttctcaa gcaaggtttt
     2101 cagtataatg ttacatgcgt acacgcgtct gtacagaaaa aaaagaaaaa tttgaaatat
     2161 aaataacgtt cttaatacta acataactat aaaaaaataa atagggacct agacttcagg
     2221 ttgtctaact ccttcctttt cggttagagc ggatgtgggg ggagggcgtg aatgtaagcg
     2281 tgacataact aattacatga ctcgaggcta gcttatcatt ttttgtgcgc cagacggttg
     2341 cgcagcgccg ctttttcttt acgcagacgc gcgatttctt tttccaggta agcgttttca
     2401 ttttcgatga acgcagcgtc attctccaga gtggtaactt cgttagatct tttaaattcc
     2461 attgtaaaag gtgctgcggc acttccagct tttttatata aggaagtttg attgctgcca
     2521 ccgtcgacac tagatccccc caccttccgc ttcttcttgg gttcagcacc agcccccacc
     2581 ttccgcttct tcttgggttc ggatctcata agctttccct gcattaaaga atcggccaaa
     2641 gccctggggg aagctgtttg agtattaggt gctagggtcg tttttctctt taccaaacta
     2701 actggaagca gccacgcacc tgatttatgg taaccacctc ttagccttag cacaagatgt
     2761 aaggtggact ccttctgaat gttgtaatca gacagcgttc taccgtcttc tagctgctta
     2821 ccggcaaaga tcaatctttg ttgatctgga gggatacctt ccttgtcttg aattttcgac
     2881 ttaacgttgt cgatggtatc ggaagattca acttccaatg ttatggtttt accggtcaaa
     2941 gtcttgacga aaatctgcat gatatcgaat tcctgcagcc cgggggatcc actagttcta
     3001 gactttatat tatcaatatt tgtgtttgtg gagggggggt gtacaatata caattgtttc
     3061 ttgatgtctt atgtaggaga gtaagataaa agaaaattaa agggagaaag aatttaaggt
     3121 aacgtaacag tttcactcat ggtagtatat atataactgc gaaactgcca ataccaagcc
     3181 agtctacgtg cgaattaggt tggatagatt tgatgaaccc attctgatac ttgttgtgcg
     3241 gaaaaaggga caaaataggg aaaactggaa aaagaaaagg gaggggagag aagcaaggcg
     3301 ggaggccgcg cgcaggaggg ccttgcaggg ggcgccccgt ttacgtattg gtggtgtggg
     3361 tggggacttt gggtgccagg ggtgtgggtg tttgatttgt ttctgaggga caatggcgcg
     3421 tgatcggcca gagacatagg gacagtagga ggacaacagt ctcattgacc ctgtcttggc
     3481 cttctgtttt tcttcttttt ttgttctttc gtgttgcgtt tgtactgacc tgatctaccg
     3541 aggacaaaac aagataggcc aaagacgata tgtgaatgga atgtttttcc gatgtagaag
     3601 tagaagtggg gttacttttt ttattatttg acgcaatgcg agaattttta tgctacgtaa
     3661 acttagactt gctcaaaccc caggagaggc ctttgtggag gagacgggga ccgctgtcac
     3721 cttgcctggg aaagagtata tattttcctt tttagtgaag ctgagtcagc ggtgagagat
     3781 gagaggtgag gggcgatgcg attacgtggt catcacgtgc gtgtcagata tactgttata
     3841 ctacgtctac acgcttggct ttcttttttc cgtttttttt ccggccctta gttaagccgg
     3901 gcagctctgc ccggctctat aggtgaaaaa aaaaaaatga agacggcaag gtcccatcag
     3961 ctgtacaaaa taatcggagt aacgataact ctttcgggct tcgagctcca attcgcccta
     4021 tagtgagtcg tattagacgt ctcccttcat ggtcatagct gtttcctgat aacttcgtat
     4081 aatgtatgct atacgaagtt attctgtagg cgaacctaac cggccaattt tccaagaccg
     4141 ctctgccgct gagcttgcac ccgtgccgct acacaacacc cacacatccg taaacccccc
     4201 gccatcggta tcaccaaggt tgtctatgac gaggcgtaca agaggacgtg ggggccgcct
     4261 ctccaatgtc accaatagga aaaaagaaag accgagtgcg agtttctgac agaagcctca
     4321 agaatttttt ttctttcttc gactatgctg gaggtagaga tgatcgaggc agtagtcaag
     4381 tatatataaa ttaattagtt ccaccacctt cttttcttgt gttgctcctt tcgatgctat
     4441 ttctggcttt ttctaatttt tttcatcgtt tcttactttc ttcctaatat ataaattctt
     4501 ttgcattttc attcttcctc tccatttgtt cattctcctt aaattttttt taggtctcct
     4561 ttctttcaag aaacacagtc atcgaactat aattaactaa acatgtcagc acctaagaag
     4621 atagtagtat tacctggaga ccacgtagga caggagataa cagcagaggc aataaaggta
     4681 ttaaaggcaa tatcagacgt aaggtcaaac gtaaagttcg acttcgagaa ccacttaata
     4741 ggaggagcag caatagacgc aacaggagta cctttacctg acgaggcatt agaggcatca
     4801 aagaaggcag acgcagtatt attaggagca gtaggaggac ctaagtgggg aacaggatca
     4861 gtaaggcctg agcagggatt attaaagata aggaaggagt tacagttata cgcaaactta
     4921 aggccttgca acttcgcatc agactcatta ttagacttat cacctataaa gcctcagttc
     4981 gcaaagggaa cagacttcgt agtagtaagg gagttagtag gaggaatata cttcggaaag
     5041 aggaaggagg acgacggaga cggagtagca tgggactcag agcagtacac agtacctgag
     5101 gtacagagga taacaaggat ggcagcattc atggcattac agcacgagcc tcctttacct
     5161 atatggtcat tagacaaggc aaacgtatta gcatcatcaa ggttatggag gaagacagta
     5221 gaggagacaa taaagaacga gttccctaca ttaaaggtac agcaccagtt aatagactca
     5281 gcagcaatga tattagtaaa gaaccctaca cacttaaacg gaataataat aacatcaaac
     5341 atgttcggag acataatatc agacgaggca tcagtaatac ctggatcatt aggattatta
     5401 ccttcagcat cattagcatc attacctgac aagaacacag cattcggatt atacgagcct
     5461 tgccacggat cagcacctga cttacctaag aacaaggtaa accctatagc aacaatatta
     5521 tcagcagcaa tgatgttaaa gttatcatta aacttacctg aggagggaaa ggcaatagag
     5581 gacgcagtaa agaaggtatt agacgcagga ataaggacag gagacttagg aggatcaaac
     5641 tcaacaacag aggtaggaga cgcagtagca gaggaggtaa agaagatatt agcatgagaa
     5701 tattgataaa tttaaatttt ggaatgaggg ttgtcctttc ctttacaata gaggcatttg
     5761 agtgagtagg ttttatcacc ttcttgctct tattaaatcg cagttgtctt tccggtatgt
     5821 gcttgactga ggaacaatat tttattcatg atacatttcc atgctacgca tatcgtcatc
     5881 cacatggaag gtaattttct tctgtggcct ttcatatagt tgtgaatggt agaacaatct
     5941 tcatataata acttcgtata atgtatgcta tacgaagtta ttaatacgac tcactatagg
     6001 gcgaattgga gctcaaaccg aaatacagcc atacgtaatt tggtggccta atatgcctgg
     6061 tgtgctggat aagatattat gttaatgaaa tatttgtacg tacatccgtt tgtgcgtatg
     6121 tatatgtgtg tgtaaaatgt acattgtcca agctttccac aactcctatt atcgctagtg
     6181 ttggcaggat tatttgtttg aaaatagcat atatctcgtt atgtgaaact ttgccaaaca
     6241 atattcctta caacatccgt gcatactcta aacccaatca ctaattttca atttttaaaa
     6301 actttatact ctgtactgcg gcaattaagg ctttcgcgtc gttgagcgcg caacactacc
     6361 gggggttcct taccgtgagc ctgcgcctct gtaggagaat gtagaggttc ccgttgaggg
     6421 aactccagcc atgggttgac gggagttccc gtttggcctg attgctcttg cgcagttgac
     6481 atctagccag aaacagtagt agtgggagtt gatattacca atatttacag gtaacctata
     6541 ttgattaact aaattatagg cgtttaaatt tttgggtggt caacaaaaga ggcgagcaac
     6601 aatttgaatt gaaacgtatg tcgcgtgccc attaggaaat gagagaatat tgaggacaac
     6661 gaggagcttc gcagtgtagg aattgagaaa gaagcgacca tttgaacaat gctgaggtta
     6721 ataagaggcc cacgataaag gcgttatatt aatcaacgtt gaacatcttc ccagaaaagt
     6781 acatgcgaat aaatcaggac cgtcgaaaga cgtacggaac atcattggca aatatacatc
     6841 gggatctgtt ggatatgctt gtcgtgatga aatactgttc tcgttgtcgg cttgataaat
     6901 tttctcccat ttaaaactct ttgaaccaaa attgtgtact aacatcaatt tgtatataaa
     6961 ccatgagtgt atttcacctt gtggtttttc acagcttttt aatttagaag aacatctaga
     7021 actagtggat cccccgggct gcaggaattc gatatcaagc ttatgagatc tgcttctaaa
     7081 ggtgaagaat tattcactgg tgttgtccca attttggttg aattagatgg tgatgttaat
     7141 ggtcacaaat tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg taaattgacc
     7201 ttaaaattta tttgtactac tggtaaattg ccagttccat ggccaacctt agtcactact
     7261 ttaggttatg gtttgatgtg ttttgctaga tacccagatc atatgaaaca acatgacttt
     7321 ttcaagtctg ccatgccaga aggttatgtt caagaaagaa ctattttttt caaagatgac
     7381 ggtaactaca agaccagagc tgaagtcaag tttgaaggtg ataccttagt taatagaatc
     7441 gaattaaaag gtattgattt taaagaagat ggtaacattt taggtcacaa attggaatac
     7501 aactataact ctcacaatgt ttacatcatg gctgacaaac aaaagaatgg tatcaaagtt
     7561 aacttcaaaa ttagacacaa cattgaagat ggttctgttc aattagctga ccattatcaa
     7621 caaaatactc caattggtga tggtccagtc ttgttaccag acaaccatta cttatcctat
     7681 caatctaaat tatccaaaga tccaaacgaa aagagagacc acatggtctt gaaagaattt
     7741 gttactgctg ctggtattac ccatggtatg gatgaattgt acaaagtcga caggtcgaat
     7801 caaacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat ggggtcccag
     7861 aaagttgcgg aactcaaaaa ccgtgttgcg gttaaactga atcgtaacga acagctgaaa
     7921 aacaaagttg aagagctgaa gaaccgtaac gcttacctca agaacgaact ggcgaccctg
     7981 gagaacgagg ttgcgcgtct ggaaaacgac gttgcagaat gataatgagc tagcctcgag
     8041 tgaaaaacct tacccccaaa tgtagtgctt tggtgcgatt ccagcatcct gtgatgttca
     8101 cctacttcaa ggttatataa ttttgtgtgt agaattgtaa aaaggatgga tagacagttc
     8161 ttgagataaa gcacagttat tttggtatca catcatcgga gtatttttca attctgattt
     8221 tttgactgga cccaacaaac aactccaatt catccaattg tccttccgta ggtaaacaac
     8281 tggaaatagg tacacttctt tctatcatca atacaacaat gttagtacga gagacttcca
     8341 gatcaggtat aacgatgccc ccagatggac atgtcctata ctcttgacat tcataaacac
     8401 agagagctga ttcaggctcg aaaatattac atcgcatcct taatcgtgct gatgattcca
     8461 cgggtactac acgtataaaa agtttgttcg tagaatactt tttggttggc aaagtcatct
     8521 gaggtaccca gcttttgttc cctttagtga gggttaattt cctgtaaact ttataaatga
     8581 aattcataat agaaacgaca cgaaattaca aaatggaata tgttcatagg gtagacgaaa
     8641 ctatatacgc aatctacata catttatcaa gaaggagaaa aaggaggatg taaaggaata
     8701 caggtaagca aattgatact aatggctcaa cgtgataagg aaaaagaatt gcactttaac
     8761 attaatattg acaaggagga gggcaccaca caaaaagtta ggtgtaacag aaaatttaat
//
