LOCUS       sequence-140109-       11798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Cudre-Mauroux C, Occhiodoro T, Konig S, Salmon P, Bernheim L, Trono 
            D
  TITLE     Lentivector-mediated transfer of Bmi-1 and telomerase in muscle 
            satellite cells yields a duchenne myoblast cell line with long-term 
            genotypic and phenotypic stability.
  JOURNAL   Hum Gene Ther. 2003 Nov 1. 14(16):1525-33.
  PUBMED    14577914
REFERENCE   2  (bases 1 to 11798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2003 Nov 1. 14(16):1525-33."
COMMENT     Sequence Label: pHR-CMV-nlsCRE
FEATURES             Location/Qualifiers
     source          1..11798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(60..80)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        65..394
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      245..380
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1528..1593
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1723..1743
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2168..2302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2205..2224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2259..2278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2470..3103
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3150..3275
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3768..4001
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4186..4230
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        4509..4812
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4813..5016
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4967..4987
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5013..5037
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5105..5125
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5126..6154
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLQ
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    6182..6770
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6235..6255)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6653..6664)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6975..7608
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9087..9105)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9088..9105)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9395..9414)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9628..9647)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9747..9769
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9807..9825)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9893..9997
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9998..10066,10067..10858)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10216..10235)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11029..11617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11518..11537
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11771..11788
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
       61 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
      121 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
      181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
      241 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
      301 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
      361 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
      421 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
      481 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
      541 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
      601 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
      661 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
      721 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
      781 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
      841 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
      901 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
      961 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
     1021 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     1081 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     1141 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     1201 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     1261 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     1321 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     1381 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     1441 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     1501 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     1561 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     1621 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     1681 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     1741 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     1801 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     1861 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
     1921 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
     1981 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
     2041 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     2101 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2161 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2221 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2281 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     2341 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     2401 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     2461 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     2521 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     2581 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     2641 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     2701 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     2761 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     2821 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     2881 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     2941 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     3001 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     3061 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     3121 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     3181 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     3241 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     3301 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     3361 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     3421 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     3481 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     3541 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     3601 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     3661 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     3721 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     3781 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     3841 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     3901 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     3961 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     4021 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     4081 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     4141 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     4201 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     4261 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     4321 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     4381 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     4441 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg ataagcttgg
     4501 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     4561 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     4621 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     4681 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     4741 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     4801 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     4861 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     4921 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     4981 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     5041 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gaggatctac
     5101 catgcccaag aagaagagga aggtgtccaa tttactgacc gtacaccaaa atttgcctgc
     5161 attaccggtc gatgcaacga gtgatgaggt tcgcaagaac ctgatggaca tgttcaggga
     5221 tcgccaggcg ttttctgagc atacctggaa aatgcttcag tccgtttgcc ggtcgtgggc
     5281 ggcatggtgc aagttgaata accggaaatg gtttcccgca gaacctgaag atgttcgcga
     5341 ttatcttcta tatcttcagg cgcgcggtct ggcagtaaaa actatccagc aacatttggg
     5401 ccagctaaac atgcttcatc gtcggtccgg gctgccacga ccaagtgaca gcaatgctgt
     5461 ttcactggtt atgcggcgga tccgaaaaga aaacgttgat gccggtgaac gtgcaaaaca
     5521 ggctctagcg ttcgaacgca ctgatttcga ccaggttcgt tcactcatgg aaaatagcga
     5581 tcgctgccag gatatacgta atctggcatt tctggggatt gcttataaca ccctgttacg
     5641 tatagccgaa attgccagga tcagggttaa agatatctca cgtactgacg gtgggagaat
     5701 gttaatccat attggcagaa cgaaaacgct ggttagcacc gcaggtgtag agaaggcact
     5761 tagcctgggg gtaactaaac tggtcgagcg atggatttcc gtctctggtg tagctgatga
     5821 tccgaataac tacctgtttt gccgggtcag aaaaaatggt gttgccgcgc catctgccac
     5881 cagccagcta tcaactcgcg ccctggaagg gatttttgaa gcaactcatc gattgattta
     5941 cggcgctaag gatgactctg gtcagagata cctggcctgg tctggacaca gtgcccgtgt
     6001 cggagccgcg cgagatatgg cccgcgctgg agtttcaata ccggagatca tgcaagctgg
     6061 tggctggacc aatgtaaata ttgtcatgaa ctatatccgt aacctggata gtgaaacagg
     6121 ggcaatggtg cgcctgctgg aagatggcga ttaggtcgag ctcaagcttc gaattcccga
     6181 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     6241 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     6301 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     6361 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     6421 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     6481 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     6541 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     6601 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     6661 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     6721 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgggaatt
     6781 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     6841 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     6901 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6961 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     7021 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     7081 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     7141 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     7201 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     7261 tcacatggcc cgagagctgc atccggagta cttcaagaac tgctgacatc gagcttgcta
     7321 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     7381 gcgagccctc agatgctgca tataagcagc tgctttttgc ttgtactggg tctctctggt
     7441 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7501 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7561 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     7621 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     7681 cttgacatta taatagattt agcaggaatt gaactaggag tggagcacac aggcaaagct
     7741 gcagaagtac ttggaagaag ccaccagaga tactcacgat tctgcacata cctggctaat
     7801 cccagatcct aaggattaca ttaagtttac taacatttat ataatgattt atagtttaaa
     7861 gtataaactt atctaattta ctattctgac agatattaat taatcctcaa atatcataag
     7921 agatgattac tattatcccc atttaacaca agaggaaact gagagggaaa gatgttgaag
     7981 taattttccc acaattacag catccgttag ttacgactct atgatcttct gacacaaatt
     8041 ccatttactc ctcaccctat gactcagtcg aatatatcaa agttatggac attatgctaa
     8101 gtaacaaatt acccttttat atagtaaata ctgagtagat tgagagaaga aattgtttgc
     8161 aaacctgaat agcttcaaga agaagagaag tgaggataag aataacagtt gtcatttaac
     8221 aagttttaac aagtaacttg gttagaaagg gattcaaatg cataaagcaa gggataaatt
     8281 tttctggcaa caagactata caatataacc ttaaatatga cttcaaataa ttgttggaac
     8341 ttgataaaac taattaaata ttattgaaga ttatcaatat tataaatgta atttactttt
     8401 aaaaagggaa catagaaatg tgtatcatta gagtagaaaa caatccttat tatcacaatt
     8461 tgtcaaaaca agtttgttat taacacaagt agaatactgc attcaattaa gttgactgca
     8521 gattttgtgt tttgttaaaa ttagaaagag ataacaacaa tttgaattat tgaaagtaac
     8581 atgtaaatag ttctacatac gttcttttga catcttgttc aatcattgat cgaagttctt
     8641 tatcttggaa gaatttgttc caaagactct gaaataagga aaacaatcta ttatatagtc
     8701 tcacaccttt gttttacttt tagtgatttc aatttaataa tgtaaatggt taaaatttat
     8761 tcttctctga gatcatttca cattgcagat agaaaacctg agactggggt aatttttatt
     8821 aaaatctaat ttaatctcag aaacacatct ttattctaac atcaattttt ccagtttgat
     8881 attatcatat aaagtcagcc ttcctcatct gcaggttcca caacaaaaat ccaaccaact
     8941 gtggatcaaa aatattggga aaaaattaaa aatagcaata caacaataaa aaaatacaaa
     9001 tcagaaaaac agcacagtat aacaacttta tttagcattt acaatctatt aggtattata
     9061 agtaatctag aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata
     9121 cataaggtta tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt
     9181 gtagcatctg gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc
     9241 ggtttggttg gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc
     9301 agcgaaccaa tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc
     9361 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     9421 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     9481 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     9541 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     9601 ggagagcgtc gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     9661 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     9721 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     9781 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     9841 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     9901 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     9961 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
    10021 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
    10081 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
    10141 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
    10201 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
    10261 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    10321 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    10381 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    10441 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    10501 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    10561 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    10621 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    10681 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    10741 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    10801 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    10861 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    10921 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    10981 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    11041 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    11101 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    11161 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    11221 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    11281 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    11341 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    11401 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    11461 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    11521 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    11581 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    11641 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    11701 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    11761 gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
