LOCUS       sequence-140110-        2426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  modified pENTR vector for C-terminal 3x HA tag fusion with your gene
            of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Dubin MJ, Bowler C, Benvenuto G
  TITLE     A modified Gateway cloning strategy for overexpressing tagged 
            proteins in plants.
  JOURNAL   Plant Methods. 2008 Jan 22. 4(1):3.
  PUBMED    18211686
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods. 2008 Jan 22. 4(1):3."
COMMENT     Sequence Label: pE2c
FEATURES             Location/Qualifiers
     source          1..2426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      59..145
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      237..264
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     272..295
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    314..413
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             489..515
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             489..515
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             516..542
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             516..542
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(611..710)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(822..841)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             833..1642
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(900..919)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1732..2320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2221..2240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctagcatgga tctcggggac gtctaactac taagcgagag tagggaactg ccaggcatca
       61 aataaaacga aaggctcagt cggaagactg ggcctttcgt tttatctgtt gtttgtcggt
      121 gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg tgaagcaacg
      181 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ctaagcagaa
      241 ggccatcctg acggatggcc tttttgcgtt tctacaaact cttcctgtta gttagttact
      301 taagctcggg ccccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
      361 aattgataag caatgctttt ttataatgcc aactttgtac aaaaaagcag gctttaaagg
      421 aaccaattca gtcgactgga tccggtacag atttcggaca tgcggccgca agcggaggcc
      481 tgtcgcgata cccctacgac gtgcccgact acgcctaccc ctacgacgtg cccgactacg
      541 ccgatcgatc cggaccgtac ccctacgacg tgcccgacta agcctagagc agtactctcg
      601 agatatctag acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
      661 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
      721 gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
      781 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
      841 tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg atttatatgg
      901 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
      961 gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     1021 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     1081 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggaaaaac
     1141 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     1201 agtgtccctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     1261 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     1321 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact
     1381 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     1441 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     1501 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     1561 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     1621 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacatt attcagattg
     1681 ggccccgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1741 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1801 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1861 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1921 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1981 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     2041 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     2101 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     2161 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     2221 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     2281 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     2341 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     2401 tgattctgtg gataaccgta ttaccg
//
