LOCUS       sequence-140122-       11893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Zhang B, Groffen J, Heisterkamp N
  TITLE     Increased resistance to a farnesyltransferase inhibitor by 
            N-cadherin expression in Bcr/Abl-P190 lymphoblastic leukemia cells.
  JOURNAL   Leukemia. 2007 Jun;21(6):1189-97. Epub 2007 Mar 29.
  PUBMED    17392819
REFERENCE   2  (bases 1 to 11893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Leukemia.";
            date: "2007-06"; volume: "21(6)"; pages: "1189-97. Epub 2007 Mar 29"
COMMENT     Sequence Label: N-cadherin in pCCL-c-MNDU3c-PGK-EGFP
FEATURES             Location/Qualifiers
     source          1..11893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        361..740
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        741..944
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     895..915
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             959..1139
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1186..1311
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1804..2037
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2222..2266
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2563..2680
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7332..7831
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7367..7390)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7764..7783
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      7852..7861
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7858..7860,7861..7863,7864..8577)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7858..7860,7861..7863,7864..8574)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7903..7924)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8164..8183)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8511..8532
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             8699..8932
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9004..9125
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9041..9060)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9095..9114
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9165..9300
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9320..9339)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9321..9339)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9349..9366)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9349..9365)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9358..9380)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9507..9962
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9594..9613)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9804..9825
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9988..10092
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10093..10161,10162..10953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10311..10330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11124..11712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11613..11632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11866..11883
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttggcca ttgcatacgt
      301 tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt
      361 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
      421 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      481 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      541 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      601 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      661 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      721 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      781 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      841 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      901 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccggg
      961 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     1021 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     1081 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     1141 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     1201 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     1261 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     1321 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     1381 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     1441 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     1501 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     1561 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     1621 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     1681 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1741 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1801 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1861 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1921 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1981 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     2041 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     2101 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2161 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2221 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2281 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2341 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2401 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2461 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     2521 gtatcgataa gctaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2581 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2641 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2701 cagagatcca gtttgggaat tagcttgatc gattagtcca atttgttaaa gacaggatat
     2761 cagtggtcca ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg
     2821 agccatagat agaataaaag attttattta gtctccagaa aaagggggga atgaaagacc
     2881 ccacctgtag gtttggcaag ctaggatcaa ggttaggaac agagagacag cagaatatgg
     2941 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagttgg
     3001 aacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     3061 gccaagaaca gatggtcccc agatgcggtc ccgccctcag cagtttctag agaaccatca
     3121 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
     3181 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
     3241 acaacccctc actcggcgcg atctagatct cgaatcgaat tggccgcgaa ttcggcacga
     3301 gggctgccgc tgcctcctcc tcctccgccg ccgccgccgc cgccgccgcc tcctccggct
     3361 cttcgctcgg cccctctccg cctccatgtg ccggatagcg ggagcgctgc ggaccctgct
     3421 gccgctgctg gcggccctgc ttcaggcgtc tgtagaggct tctggtgaaa tcgcattatg
     3481 caagactgga tttcctgaag atgtttacag tgcagtctta tcgaaggatg tgcatgaagg
     3541 acagcctctt ctcaatgtga agtttagcaa ctgcaatgga aaaagaaaag tacaatatga
     3601 gagcagtgag cctgcagatt ttaaggtgga tgaagatggc atggtgtatg ccgtgagaag
     3661 ctttccactc tcttctgagc atgccaagtt cctgatatat gcccaagaca aagagaccca
     3721 ggaaaagtgg caagtggcag taaaattgag cctgaagcca accttaactg aggagtcagt
     3781 gaaggagtca gcagaagttg aagaaatagt gttcccaaga caattcagta agcacagtgg
     3841 ccacctacaa aggcagaaga gagactgggt catccctcca atcaacttgc cagaaaactc
     3901 caggggacct tttcctcaag agcttgtcag gatcaggtct gatagagata aaaacctttc
     3961 actgcggtac agtgtaactg ggccaggagc tgaccagcct ccaactggta tcttcattat
     4021 caaccccatc tcgggtcagc tgtcggtgac aaagcccctg gatcgcgagc agatagcccg
     4081 gtttcatttg agggcacatg cagtagatat taatggaaat caagtggaga accccattga
     4141 cattgtcatc aatgttattg acatgaatga caacagacct gagttcttac accaggtttg
     4201 gaatgggaca gttcctgagg gatcaaagcc tggaacatat gtgatgaccg taacagcaat
     4261 tgatgctgac gatcccaatg ccctcaatgg gatgttgagg tacagaatcg tgtctcaggc
     4321 tccaagcacc ccttcaccca acatgtttac aatcaacaat gagactggtg acatcatcac
     4381 agtggcagct ggacttgatc gagaaaaagt gcaacagtat acgttaataa ttcaagctac
     4441 agacatggaa ggcaatccca catatggcct ttcaaacaca gccacggccg tcatcacagt
     4501 gacagatgtc aatgacaatc ctccagagtt tactgccatg acgttttatg gtgaagttcc
     4561 tgagaacagg gtagacatca tagtagctaa tctaactgtg accgataagg atcaacccca
     4621 tacaccagcc tggaacgcag tgtacagaat cagtggcgga gatcctactg gacggttcgc
     4681 catccagacc gacccaaaca gcaacgacgg gttagtcacc gtggtcaaac caatcgactt
     4741 tgaaacaaat aggatgtttg tccttactgt tgctgcagaa aatcaagtgc cattagccaa
     4801 gggaattcag cacccgcctc agtcaactgc aaccgtgtct gttacagtta ttgacgtaaa
     4861 tgaaaaccct tattttgccc ccaatcctaa gatcattcgc caagaagaag ggcttcatgc
     4921 cggtaccatg ttgacaacat tcactgctca ggacccagat cgatatatgc agcaaaatat
     4981 tagatacact aaattatctg atcctgccaa ttggctaaaa atagatcctg tgaatggaca
     5041 aataactaca attgctgttt tggaccgaga atcaccaaat gtgaaaaaca atatatataa
     5101 tgctactttc cttgcttctg acaatggaat tcctcctatg agtggaacag gaacgctgca
     5161 gatctattta cttgatatta atgacaatgc ccctcaagtg ttacctcaag aggcagagac
     5221 ttgcgaaact ccagacccca attcaattaa tattacagca cttgattatg acattgatcc
     5281 aaatgctgga ccatttgctt ttgatcttcc tttatctcca gtgactatta agagaaattg
     5341 gaccatcact cggcttaatg gtgattttgc tcagcttaat ttaaagataa aatttcttga
     5401 agctggtatc tatgaagttc ccatcataat cacagattcg ggtaatcctc ccaaatcaaa
     5461 tatttccatc ctgcgcgtga aggtttgcca gtgtgactcc aacggggact gcacagatgt
     5521 ggacaggatt gtgggtgcgg ggcttggcac cggtgccatc attgccatcc tgctctgcat
     5581 catcatcctg cttatccttg tgctgatgtt tgtggtatgg atgaaacgcc gggataaaga
     5641 acgccaggcc aaacaacttt taattgatcc agaagatgat gtaagagata atattttaaa
     5701 atatgatgaa gaaggtggag gagaagaaga ccaggactat gacttgagcc agctgcagca
     5761 gcctgacact gtggagcctg atgccatcaa gcctgtggga atccgacgaa tggatgaaag
     5821 acccatccac gccgagcccc agtatccggt ccgatctgca gccccacacc ctggagacat
     5881 tggggacttc attaatgagg gccttaaagc ggctgacaat gaccccacag ctccaccata
     5941 tgactccctg ttagtgtttg actatgaagg cagtggctcc actgctgggt ccttgagctc
     6001 ccttaattcc tcaagtagtg gtggtgagca ggactatgat tacctgaacg actgggggcc
     6061 acggttcaag aaacttgctg acatgtatgg tggaggtgat gactgaactt cagggtgaac
     6121 ttggtttttg gacaagtaca aacaatttca actgatattc ccaaaaagca ttcagaagct
     6181 aggctttaac tttgtagtct actagcacag tgcttgctgg aggctttggc ataggctgca
     6241 aaccaatttg ggctcagagg gaatatcagt gatccatact gtttggaaaa acactgagct
     6301 cagttacact tgaattttac agtacagaag cactgggatt ttatgtgcct ttttgtacct
     6361 ttttcagatt ggaattagtt ttctgtttaa ggctttaatg gtactgattt ctgaaacgat
     6421 aagtaaaaga caaaatattt tgtggtggga gcagtaagtt aaaccatgat atgcttcaac
     6481 acgcttttgt tacattgcat ttgcttttat taaaatacaa aattaaacaa acaaaaaaac
     6541 tcatggagcg attttattat cttgggggat gagaccatga gattggaaaa tgtacattac
     6601 ttctagtttt agactttagt ttgttttttt tttttcacta aaatcttaaa acttactcag
     6661 ctggttgcaa ataaagggag ttttcatatc accaatttgt agcaaaattg aattttttca
     6721 taaactagaa tgttagacac attttggtct taatccatgt acactttttt atttctgtat
     6781 ttttccactt cactgtaaaa atagtatgtg tacataatgt tttattggca tagtctatgg
     6841 agaagtgcag aaacttcaga acatgtgtat gtattatttg gactatggat tcaggttttt
     6901 tgcatgttta tatctttcgt tatggataaa gtatttacaa aacagtgaca tttgattcaa
     6961 ttgttgagct gtagttagaa tactcaattt ttaatttttt taattttttt attttttatt
     7021 ttctttttgg tttggggagg gagaaaagtt cttagcacaa atgttttaca taatttgtac
     7081 caaaaaaaaa aaaaaggaaa ggaaagaaag gggtggcctg acactggtgg cactactaag
     7141 tgtgtgtttt tttaaaaaaa aaatggaaaa aaaaagcttt taaactggag agacttctga
     7201 caacagcttt gcctctgtat tgtgtaccag aatataaatg atacacctct gaccccagcg
     7261 ttctgaataa aatgctaatt ttggaaaaaa aaaaaaaaaa aactcgactc tagattgcgg
     7321 ccaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     7381 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     7441 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     7501 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     7561 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     7621 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     7681 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     7741 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     7801 gttctcctct tcctcatctc cgggcctttc gaccatctag atccaccggt cgccaccatg
     7861 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     7921 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     7981 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     8041 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     8101 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     8161 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     8221 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     8281 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     8341 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     8401 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     8461 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     8521 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     8581 ggccaactcg agggatcccc cggggtcgac tgatcaaatt cgagctcggt acctttaaga
     8641 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8701 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     8761 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8821 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8881 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     8941 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     9001 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9061 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9121 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     9181 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     9241 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     9301 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     9361 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     9421 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     9481 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     9541 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     9601 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     9661 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     9721 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     9781 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     9841 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     9901 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     9961 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    10021 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    10081 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    10141 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    10201 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    10261 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    10321 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    10381 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    10441 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    10501 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    10561 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    10621 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    10681 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    10741 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    10801 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    10861 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    10921 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    10981 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    11041 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    11101 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    11161 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    11221 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    11281 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    11341 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    11401 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    11461 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    11521 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    11581 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    11641 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    11701 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    11761 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    11821 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    11881 gaggaagcgg aag
//
