LOCUS       sequence-140124-        8646 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of human TERT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   Sexton AN, Collins K
  TITLE     The 5' guanosine tracts of human telomerase RNA are recognized by 
            the G-quadruplex binding domain of the RNA helicase DHX36 and 
            function to increase RNA accumulation.
  JOURNAL   Mol Cell Biol. 2011 Feb;31(4):736-43. doi: 10.1128/MCB.01033-10. 
            Epub 2010 Dec 13.
  PUBMED    21149580
REFERENCE   2  (bases 1 to 8646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01033-10"; journalName: "Mol Cell Biol"; date: 
            "2011-02"; volume: "31"; issue: "4"; pages: "736-43"
COMMENT     Sequence Label: pBABE Hygro Flag hTERT
FEATURES             Location/Qualifiers
     source          1..8646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             98..567
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    632..831
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1032..1448
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1378..1400
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1416..1432
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1490..1513
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1523..4921
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     primer_bind     complement(4933..4953)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4938..5267
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5118..5253
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5341..6366
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5341..6366
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     6883..6902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             7164..7633
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(7748..8539,8540..8608))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8371..8390
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(8609..8646,1..67))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
       61 ttccgcgcac atttccccga aaagtgccac ctgcagcctg aatatgggcc aaacaggata
      121 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat
      181 gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      241 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
      301 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
      361 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
      421 cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
      481 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      541 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
      601 cccagggacc accgacccac caccgggagg caagctggcc agcaacttat ctgtgtctgt
      661 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
      721 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc
      781 ctgggagacg tcccagggac tttgggggcc gtttttgtgg cccgacctga ggaagggagt
      841 cgatgtggaa tccgaccccg tcaggatatg tggttctggt aggagacgag aacctaaaac
      901 agttcccgcc tccgtctgaa tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc
      961 tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
     1021 ctgaaaatta gggccagact gttaccactc ccttaagttt gaccttaggt cactggaaag
     1081 atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa gaagagacgt tgggttacct
     1141 tctgctctgc agaatggcca acctttaacg tcggatggcc gcgagacggc acctttaacc
     1201 gagacctcat cacccaggtt aagatcaagg tcttttcacc tggcccgcat ggacacccag
     1261 accaggtccc ctacatcgtg acctgggaag ccttggcttt tgacccccct ccctgggtca
     1321 agccctttgt acaccctaag cctccgcctc ctcttcctcc atccgccccg tctctccccc
     1381 ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta tccagccctc actccttctc
     1441 taggcgccgg ccggatccca gtgtggtggt acagcttggt accaacatgg actacaagga
     1501 cgacgatgac aagggaattc atatgccgcg cgctccccgc tgccgagccg tgcgctccct
     1561 gctgcgcagc cactaccgcg aggtgctgcc gctggccacg ttcgtgcggc gcctggggcc
     1621 ccagggctgg cggctggtgc agcgcgggga cccggcggct ttccgcgcgc tggtggccca
     1681 gtgcctggtg tgcgtgccct gggacgcacg gccgcccccc gccgccccct ccttccgcca
     1741 ggtgtcctgc ctgaaggagc tggtggcccg agtgctgcag aggctgtgcg agcgcggcgc
     1801 gaagaacgtg ctggccttcg gcttcgcgct gctggacggg gcccgcgggg gcccccccga
     1861 ggccttcacc accagcgtgc gcagctacct gcccaacacg gtgaccgacg cactgcgggg
     1921 gagcggggcg tgggggctgc tgctgcgccg cgtgggcgac gacgtgctgg ttcacctgct
     1981 ggcacgctgc gcgctctttg tgctggtggc tcccagctgc gcctaccagg tgtgcgggcc
     2041 gccgctgtac cagctcggcg ctgccactca ggcccggccc ccgccacacg ctagtggacc
     2101 ccgaaggcgt ctgggatgcg aacgggcctg gaaccatagc gtcagggagg ccggggtccc
     2161 cctgggcctg ccagccccgg gtgcgaggag gcgcgggggc agtgccagcc gaagtctgcc
     2221 gttgcccaag aggcccaggc gtggcgctgc ccctgagccg gagcggacgc ccgttgggca
     2281 ggggtcctgg gcccacccgg gcaggacgcg tggaccgagt gaccgtggtt tctgtgtggt
     2341 gtcacctgcc agacccgccg aagaagccac ctctttggag ggtgcgctct ctggcacgcg
     2401 ccactcccac ccatccgtgg gccgccagca ccacgcgggc cccccatcca catcgcggcc
     2461 accacgtccc tgggacacgc cttgtccccc ggtgtacgcc gagaccaagc acttcctcta
     2521 ctcctcaggc gacaaggagc agctgcggcc ctccttccta ctcagctctc tgaggcccag
     2581 cctgactggc gctcggaggc tcgtggagac catctttctg ggttccaggc cctggatgcc
     2641 agggactccc cgcaggttgc cccgcctgcc ccagcgctac tggcaaatgc ggcccctgtt
     2701 tctggagctg cttgggaacc acgcgcagtg cccctacggg gtgctcctca agacgcactg
     2761 cccgctgcga gctgcggtca ccccagcagc cggtgtctgt gcccgggaga agccccaggg
     2821 ctctgtggcg gcccccgagg aggaggacac agacccccgt cgcctggtgc agctgctccg
     2881 ccagcacagc agcccctggc aggtgtacgg cttcgtgcgg gcctgcctgc gccggctggt
     2941 gcccccaggc ctctggggct ccaggcacaa cgaacgccgc ttcctcagga acaccaagaa
     3001 gttcatctcc ctggggaagc atgccaagct ctcgctgcag gagctgacgt ggaagatgag
     3061 cgtgcgggac tgcgcttggc tgcgcaggag cccaggggtt ggctgtgttc cggccgcaga
     3121 gcaccgtctg cgtgaggaga tcctggccaa gttcctgcac tggctgatga gtgtgtacgt
     3181 cgtcgagctg ctcaggtctt tcttttatgt cacggagacc acgtttcaaa agaacaggct
     3241 ctttttctac cggaagagtg tctggagcaa gttgcaaagc attggaatca gacagcactt
     3301 gaagagggtg cagctgcggg agctgtcgga agcagaggtc aggcagcatc gggaagccag
     3361 gcccgccctg ctgacgtcca gactccgctt catccccaag cctgacgggc tgcggccgat
     3421 tgtgaacatg gactacgtcg tgggagccag aacgttccgc agagaaaaga gggccgagcg
     3481 tctcacctcg agggtgaagg cactgttcag cgtgctcaac tacgagcggg cgcggcgccc
     3541 cggcctcctg ggcgcctctg tgctgggcct ggacgatatc cacagggcct ggcgcacctt
     3601 cgtgctgcgt gtgcgggccc aggacccgcc gcctgagctg tactttgtca aggtggatgt
     3661 gacgggcgcg tacgacacca tcccccagga caggctcacg gaggtcatcg ccagcatcat
     3721 caaaccccag aacacgtact gcgtgcgtcg gtatgccgtg gtccagaagg ccgcccatgg
     3781 gcacgtccgc aaggccttca agagccacgt ctctaccttg acagacctcc agccgtacat
     3841 gcgacagttc gtggctcacc tgcaggagac cagcccgctg agggatgccg tcgtcatcga
     3901 gcagagctcc tccctgaatg aggccagcag tggcctcttc gacgtcttcc tacgcttcat
     3961 gtgccaccac gccgtgcgca tcaggggcaa gtcctacgtc cagtgccagg ggatcccgca
     4021 gggctccatc ctctccacgc tgctctgcag cctgtgctac ggcgacatgg agaacaagct
     4081 gtttgcgggg attcggcggg acgggctgct cctgcgtttg gtggatgatt tcttgttggt
     4141 gacacctcac ctcacccacg cgaaaacctt cctcaggacc ctggtccgag gtgtccctga
     4201 gtatggctgc gtggtgaact tgcggaagac agtggtgaac ttccctgtag aagacgaggc
     4261 cctgggtggc acggcttttg ttcagatgcc ggcccacggc ctattcccct ggtgcggcct
     4321 gctgctggat acccggaccc tggaggtgca gagcgactac tccagctatg cccggacctc
     4381 catcagagcc agtctcacct tcaaccgcgg cttcaaggct gggaggaaca tgcgtcgcaa
     4441 actctttggg gtcttgcggc tgaagtgtca cagcctgttt ctggatttgc aggtgaacag
     4501 cctccagacg gtgtgcacca acatctacaa gatcctcctg ctgcaggcgt acaggtttca
     4561 cgcatgtgtg ctgcagctcc catttcatca gcaagtttgg aagaacccca catttttcct
     4621 gcgcgtcatc tctgacacgg cctccctctg ctactccatc ctgaaagcca agaacgcagg
     4681 gatgtcgctg ggggccaagg gcgccgccgg ccctctgccc tccgaggccg tgcagtggct
     4741 gtgccaccaa gcattcctgc tcaagctgac tcgacaccgt gtcacctacg tgccactcct
     4801 ggggtcactc aggacagccc agacgcagct gagtcggaag ctcccgggga cgacgctgac
     4861 tgccctggag gccgcagcca acccggcact gccctcagac ttcaagacca tcctggactg
     4921 agtcgaccct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     4981 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     5041 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     5101 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5161 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5221 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttgccagct
     5281 ggggcgccct ctggtaaggt tgggaagccc tgcagatctg atcccggggg gcaatgagat
     5341 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     5401 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     5461 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     5521 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     5581 ggggaattta gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     5641 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     5701 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     5761 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     5821 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     5881 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     5941 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     6001 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     6061 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     6121 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     6181 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     6241 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     6301 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     6361 gaatagagta gatgccgacc gggatctatc gatgcggcgg gtgtggtggt tacgcgtaat
     6421 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6481 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6541 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6601 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6661 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6721 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6781 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6841 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6901 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6961 aggggggcgg agcctatgga aaaacgccag caacgcatcg ataaaataaa agattttatt
     7021 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
     7081 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc
     7141 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
     7201 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
     7261 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
     7321 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
     7381 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
     7441 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc
     7501 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
     7561 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
     7621 cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt
     7681 acattaaatg gccatagtgc ggccgcaaat caatctaaag tatatatgag taaacttggt
     7741 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7801 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7861 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7921 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7981 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8041 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8101 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8161 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8221 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8281 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8341 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8401 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8461 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8521 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8581 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8641 atcagg
//
