LOCUS       sequence-140125-        6261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses constitutively active human BMP receptor 1A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Varley JE, McPherson CE, Zou H, Niswander L, Maxwell GD
  TITLE     Expression of a constitutively active type I BMP receptor using a 
            retroviral vector promotes the development of adrenergic cells in 
            neural crest cultures.
  JOURNAL   Dev Biol. 1998 Apr 1;196(1):107-18.
  PUBMED    9527884
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "1998-04-1"; volume: "196(1)"; pages: "107-18"
COMMENT     Sequence Label: pCMV5-hBMPR-1A CA
FEATURES             Location/Qualifiers
     source          1..6261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2285..2907
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2484..2503)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2931..2951)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2936..3265
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3116..3251
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3303..3322)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3304..3322)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3336..3352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3336..3352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3349..3371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3360..3376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3384..3390,3391..3408,3409..3414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3429..3450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3567..3584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3738..4326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3818..3837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4497..5288,5289..5357))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5120..5139
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5358..5462)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5489..5944
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5576..5595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5786..5807
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6070..6092
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6084..6101
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6085..6101
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattcaga tctggtacca cgcgtatcga taagcttaca attgaaccat ggctcagcta
      661 tacatttaca tcagattatt gggagcctat ttgttcatca tttctcgtgt tcaaggacag
      721 aatctggata gtatgcttca tggcactggg atgaaatcag actccgacca gaaaaagtca
      781 gaaaatggag taaccttagc accagaggat accttgcctt ttttaaagtg ctattgctca
      841 gggcactgtc cagatgatgc tattaataac acatgcataa ctaatggaca ttgctttgcc
      901 atcatagaag aagatgacca gggagaaacc acattagctt cagggtgtat gaaatatgaa
      961 ggatctgatt ttcagtgcaa agattctcca aaagcccagc tacgccggac aatagaatgt
     1021 tgtcggacca atttatgtaa ccagtatttg caacccacac tgccccctgt tgtcataggt
     1081 ccgttttttg atggcagcat tcgatggctg gttttgctca tttctatggc tgtctgcata
     1141 attgctatga tcatcttctc cagctgcttt tgttacaaac attattgcaa gagcatctca
     1201 agcagacgtc gttacaatcg tgatttggaa caggatgaag catttattcc agttggagaa
     1261 tcactaagag accttattga ccagtcacaa agttctggta gtgggtctgg actaccttta
     1321 ttggttcagc gaactattgc caaagatatt cagatggtcc ggcaagttgg taaaggccga
     1381 tatggagaag tatggatggg caaatggcgt ggcgaaaaag tggcggtgaa agtattcttt
     1441 accactgaag aagccagctg gtttcgagaa acagaaatct accaaactgt gctaatgcgc
     1501 catgaaaaca tacttggttt catagcggca gacattaaag gtacaggttc ctggactcag
     1561 ctctatttga ttactgatta ccatgaaaat ggatctctct atgacttcct gaaatgtgct
     1621 acactggaca ccagagccct gcttaaattg gcttattcag ctgcctgtgg tctgtgccac
     1681 ctgcacacag aaatttatgg cacccaagga aagcccgcaa ttgctcatcg agacctaaag
     1741 agcaaaaaca tcctcatcaa gaaaaatggg agttgctgca ttgctgacct gggccttgct
     1801 gttaaattca acagtgacac aaatgaagtt gatgtgccct tgaataccag ggtgggcacc
     1861 aaacgctaca tggctcccga agtgctggac gaaagcctga acaaaaacca cttccagccc
     1921 tacatcatgg ctgacatcta cagcttcggc ctaatcattt gggagatggc tcgtcgttgt
     1981 atcacaggag ggatcgtgga agaataccaa ttgccatatt acaacatggt accgagtgat
     2041 ccgtcatacg aagatatgcg tgaggttgtg tgtgtcaaac gtttgcggcc aattgtgtct
     2101 aatcggtgga acagtgatga atgtctacga gcagttttga agctaatgtc agaatgctgg
     2161 gcccacaatc cagcctccag actcacagca ttgagaatta agaagacgct tgccaagatg
     2221 gttgaatccc aagatgtaaa aatctgatgg ttaaaccatc ggaggagaaa ctctagagga
     2281 tcccgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac
     2341 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt
     2401 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa
     2461 gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc
     2521 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga
     2581 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag
     2641 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc
     2701 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct
     2761 tctgatttta aaataactat accagcagga ggacgtccag acacagcata ggctacctgg
     2821 ccatgcccaa ccggtgggac atttgagttg cttgcttggc actgtcctct catgcgttgg
     2881 gtccactcag tagatgcctg ttgaattggg tacgcggcca gcttggctgt ggaatgtgtg
     2941 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3001 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3061 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     3121 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3181 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     3241 ttttggaggc ctaggctttt gcaaaaagct cctcgaggaa ctgaaaaacc agaaagttaa
     3301 ttccctatag tgagtcgtat taaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     3361 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3421 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     3481 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3541 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4141 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4621 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4801 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4861 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4921 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4981 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5041 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5101 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5161 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5221 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5281 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5341 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5401 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5461 cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg
     5521 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5581 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5641 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5701 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5761 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5821 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5881 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     5941 atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     6001 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     6061 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg atctatacat
     6121 tgaatcaata ttggcaatta gccatattag tcattggtta tatagcataa atcaatattg
     6181 gctattggcc attgcatacg ttgtatctat atcataatat gtacatttat attggctcat
     6241 gtccaatatg accgccatgt t
//
