LOCUS       sequence-140132-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Chen CZ, Li L, Lodish HF, Bartel DP
  TITLE     MicroRNAs modulate hematopoietic lineage differentiation.
  JOURNAL   Science. 2004 Jan 2. 303(5654):83-6.
  PUBMED    14657504
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2004 Jan 2. 303(5654):83-6."
COMMENT     Sequence Label: MDH1-miR-30-PGK-GFP
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           138..152
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(477..498)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     826..845
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atcttataag ttctgtatga gaccactcga gtggaaacac ttgctgggat tacttcttca
       61 ggttaaccca acagaaggct aaagaaggta tattgctgtt gacagtgagc gactgtaaac
      121 atcctcgact ggaagctgtg aagccacaga tgggctttca gtcggatgtt tgcagctgcc
      181 tactgcctcg gacttcaagg ggctacttta ggagcaatta tcttgtttac taaaactgaa
      241 taccttgcta tctctttgat acatttttac aaagctgaat taaaatggta taaattttta
      301 attaagaatt cttgccaaac ctacaggtgg ggtctttcat tccccccttt ttctggagac
      361 taaataaaat cttttatttt atcgataagc ttggctgcag gtcgacgcgg ccgcaattct
      421 taattaattc attacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      481 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
      541 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      601 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
      661 gccttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
      721 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      781 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      841 tcgtccttga agaa
//
