LOCUS       sequence-140145-         678 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  vector encoding both shRNA against CDK4(1) and shRNA against 
            CDK6(1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Acevedo M, Vernier M, Mignacca L, Lessard F, Huot G, Moiseeva O, 
            Bourdeau V, Ferbeyre G
  TITLE     A CDK4/6-Dependent Epigenetic Mechanism Protects Cancer Cells from 
            PML-induced Senescence.
  JOURNAL   Cancer Res. 2016 Jun 1;76(11):3252-64. doi: 
            10.1158/0008-5472.CAN-15-2347. Epub 2016 Mar 29.
  PUBMED    27206849
REFERENCE   2  (bases 1 to 678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-15-2347"; journalName: "Cancer Res"; date: 
            "2016-06-1- 1"; volume: "76"; issue: "11"; pages: "3252-64"
COMMENT     Sequence Label: shCDK4/6(1)
FEATURES             Location/Qualifiers
     source          1..678
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           4..125
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           153..167
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           195..321
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           358..479
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           507..521
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           549..675
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 tgtttgaatg aggcttcagt actttacaga atcgttgcct gcacatcttg gaaacacttg
       61 ctgggattac ttcttcaggt taacccaaca gaaggctcga gaaggtatat tgctgttgac
      121 agtgagcgta gtcttgctcc agtccagcta tagtgaagcc acagatgtat agctggactg
      181 gagcaagact ttgcctactg cctcggaatt caaggggcta ctttaggagc aattatcttg
      241 tttactaaaa ctgaatacct tgctatctct ttgatacatt tttacaaagc tgaattaaaa
      301 tggtataaat taaatcactt ttttggatct ctcgactagg gataacaggg taattgtttg
      361 aatgaggctt cagtacttta cagaatcgtt gcctgcacat cttggaaaca cttgctggga
      421 ttacttcttc aggttaaccc aacagaaggc tcgagaaggt atattgctgt tgacagtgag
      481 cgcctgagaa tggctacctc tcgatagtga agccacagat gtatcgagag gtagccattc
      541 tcagatgcct actgcctcgg aattcaaggg gctactttag gagcaattat cttgtttact
      601 aaaactgaat accttgctat ctctttgata catttttaca aagctgaatt aaaatggtat
      661 aaattaaatc actttttt
//
