LOCUS       sequence-140149-         963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human Plekhm1 in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 963)
  TITLE     Plekhm1 pFastHFTb
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Plekhm1 pFastHFTb
FEATURES             Location/Qualifiers
     source          1..963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             8..25
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             26..49
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             65..85
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gtactaccat caccatcacc atcacgatta caaagatgat gatgataaaa atatcccaac
       61 gaccgaaaac ctgtattttc agggcgccat gggatccgga attcaaaggc ctacgtcgac
      121 gagctcacta gtcgcggccg ctttcgaatc tagaatgctt tcagtggtgg agaatggact
      181 ggacccccag gctgccatcc cggtcatcaa gaagaagctg gtgggatccg tgaaggcctt
      241 gcagaagcag tacgtgtccc tggacacggt ggtcactagt gaagacggag atgccaacac
      301 catgtgcagc gccctggagg ccgtatttat ccatggcctg cacgccaagc acatccgagc
      361 tgaggccgga ggaaaaagga agaaaagtgc ccaccagaag cctctgcccc agcctgtctt
      421 ctggcccctc ctgaaagctg tcacccacaa acacatcatc tcagagttgg agcacctgac
      481 gtttgtcaac acggatgtgg gccgctgccg ggcatggctg cggctggccc tgaacgatgg
      541 cctgatggag tgctacctga agctgctgct gcaggagcag gcccgcttgc atgagtacta
      601 ccagcccacc gccctgctcc gggatgctga ggagggcgag ttcctcctta gcttcctgca
      661 gggcctcacg tccttgtcct tcgaactctc ctacaagtct gccatcttaa atgagtggac
      721 gctcacccca ttggccctgt ctgggctttg cccgctttct gagctggacc ctctctctac
      781 ctctggtgca gaactacagc ggaaggaatc tctggattcc atttcccatt cttcaggctc
      841 tgaagacatc gaagtccatc actcgggcca taagatacgg aggaaccaga agctgactgc
      901 ctcctccctc agcctggaca cggccagttc atcccagctg tcctgcagcc taaactctga
      961 tag
//
