LOCUS       sequence-140154-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human Plekhm1 N-part in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  TITLE     Plekhm1 pFastHFTb
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Plekhm1-N terminal pFastHFTb
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..19
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             20..43
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             59..79
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 ccatcaccat caccatcacg attacaaaga tgatgatgat aaaaatatcc caacgaccga
       61 aaacctgtat tttcagggcg ccatgggatc cggaattcaa aggcctacgt cgacgagctc
      121 actagtcgcg gccgctttcg aatctagaat gctttcagtg gtggagaatg gactggaccc
      181 ccaggctgcc atcccggtca tcaagaagaa gctggtggga tccgtgaagg ccttgcagaa
      241 gcagtacgtg tccctggaca cggtggtcac tagtgaagac ggagatgcca acaccatgtg
      301 cagcgccctg gaggccgtat ttatccatgg cctgcacgcc aagcacatcc gagctgaggc
      361 cggaggaaaa aggaagaaaa gtgcccacca gaagcctctg ccccagcctg tcttctggcc
      421 cctcctgaaa gctgtcaccc acaaacacat catctcagag ttggagcacc tgacgtttgt
      481 caacacggat gtgggccgct gccgggcatg gctgcggctg gccctgaacg atggcctgat
      541 ggagtgctac ctgaagctgc tgctgcagga gcaggcccgc ttgcatgagt actaccagcc
      601 caccgccctg ctccgggatg ctgaggaggg cgagttcctc cttagcttcc tgcagggcct
      661 cacgtccttg tccttcgaac tctcctacaa gtctgccatc ttaaatgagt ggacgctcac
      721 cccattggcc ctgtctgggc tttgcccgct ttctgagctg gaccctctct ctacctctgg
      781 tgcagaacta cagcggaagg aatctctgga ttccatttcc cattcttcag gctctgaaga
      841 catcgaagtc catcactcgg gccataagat acggaggaac cagaagctga ctgcctcctc
      901 cctcagcctg gacacggcca gttcatccca gctgtcctgc agcctaaact ctgatag
//
