LOCUS       sequence-140160-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human Plekhm1 C-part in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Plekhm1 pFastHFTb
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Plekhm1 - C terminal pFastHFTb
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..20
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             21..44
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             60..80
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 accatcacca tcaccatcac gattacaaag atgatgatga taaaaatatc ccaacgaccg
       61 aaaacctgta ttttcagggc gccatgggat ccggaattca aaggcctacg tcgacgagct
      121 cactagtcgc ggccgctttc gaatctagag cgttagacca agcgtgtgta ccttccccag
      181 gaagaaggca agcccaggca gccccatccc aggggcataa gagcttccgg gtggtacacc
      241 ggagacagat gggactgtcc aacccattcc ggggtctcat gaagctgggc accgtggagc
      301 ggcggggggc aatgggcatc tggaaggagc tcttctgcga gctctccccg ctggagttcc
      361 gcctctacct gagcaacgag gagcacacct gtgtggagaa ctgctcgctg cttcgctgtg
      421 agtctgtggg gccagcccat agtgatgggc gctttgagct ggtcttctct ggcaagaagc
      481 tggccctgcg cgcctcctcc caggacgaag ctgaggactg gctggaccgg gtgcgggagg
      541 ccctgcagaa ggtccggcct cagcaggagg atgagtgggt gaacgtgcag tacccagacc
      601 agcctgagga accccccgag gcgccccagg gctgcctctc tccctcagac ctgctctcgg
      661 agcccgcggc cctccagggc acacagtttg actggtcgtc cgcccaggtt ccagagccag
      721 atgccatcaa ggagtccctg ctgtacttgt acatggacag gacctggatg ccctatatat
      781 tttctctgtc cttggaggct ctgaaatgtt tccgcatcag gaacaatgag aagatgctga
      841 gtgacagcca cgg
//
