LOCUS       sequence-140163-        1755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. 
            Integrates in the URA locus of W303 and recovers URA function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Gander MW, Vrana JD, Voje Jr WE, Carothers JM, Klavins E
  TITLE     Robust digital logic circuits in eukaryotic cells with CRISPR/dCas9 
            NOR gates
  JOURNAL   bioRxiv 041871
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            041871"
COMMENT     Sequence Label: pMOD6-pGRR-r11-r10-yeGFP
FEATURES             Location/Qualifiers
     source          1..1755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     733..749
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     733..749
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             756..1472
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1525..1543)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     terminator      1555..1744
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
ORIGIN
        1 agtttatcat tatcaatact cgccatttca aagaatacgt aaataattaa tagtagtgat
       61 tttcctaact ttatttagtc aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc
      121 ccaaaatagg gggcgggtta cacagaatat ataacatcgt aggtgtctgg gtgaacagtt
      181 tattcctggc atccactaaa tataatggag cccgcttttt aagctggcat ccagaaaaaa
      241 aaagaatccc agcaccaaaa tattgttttc ttcaccaacc atcagttcat aggtccattc
      301 tcttagcgca actacagaga acaggggcac aaacaggcaa aaaacgggca caacctcaat
      361 ggagtgatgc aacctgcctg gagtaaatga tgacacaagg caattgaccc acgcatgtat
      421 ctatctcatt ttcttacacc ttctattacc ttctgctctc tctgatttgg aaaaagctga
      481 aaaaaaaggt tgaaaccagt tccctgaaat tattccccta cttgactaat aagtaaattc
      541 ctgcagcccg ggtactgtat gggcaaagag acgcttgtcg tggcatgcat gtgctctgta
      601 tgtatataaa actcttgttt tcttcttttc tctaaatatt ctttcctgag tcgaatgttt
      661 atgtcacgca taaattacta tacttctata gacacacaaa cacaaataca cacactaatc
      721 tagatattgg attctagaac tagtggatct acaaaatgtc taaaggtgaa gaattattca
      781 ctggtgttgt cccaattttg gttgaattag atggtgatgt taatggtcac aaattttctg
      841 tctccggtga aggtgaaggt gatgctactt acggtaaatt gaccttaaaa tttatttgta
      901 ctactggtaa attgccagtt ccatggccaa ccttagtcac tactttcggt tatggtgttc
      961 aatgttttgc tagataccca gatcatatga aacaacatga ctttttcaag tctgccatgc
     1021 cagaaggtta tgttcaagaa agaactattt ttttcaaaga tgacggtaac tacaagacca
     1081 gagctgaagt caagtttgaa ggtgatacct tagttaatag aatcgaatta aaaggtattg
     1141 attttaaaga agatggtaac attttaggtc acaaattgga atacaactat aactctcaca
     1201 atgtttacat catggctgac aaacaaaaga atggtatcaa agttaacttc aaaattagac
     1261 acaacattga agatggttct gttcaattag ctgaccatta tcaacaaaat actccaattg
     1321 gtgatggtcc agtcttgtta ccagacaacc attacttatc cactcaatct gccttatcca
     1381 aagatccaaa cgaaaagaga gaccacatgg tcttgttaga atttgttact gctgctggta
     1441 ttacccatgg tatggatgaa ttgtacaaat aatgataccg tcgacctcga gtctgatacc
     1501 gtcgacctcg agtcaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc
     1561 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat
     1621 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag
     1681 acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc
     1741 gaaggcttta atttg
//
